Ankle2 (NM_001253814) Mouse Tagged ORF Clone

CAT#: MR231437

Ankle2 (myc-DDK-tagged) - Mouse ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 1

Product Images

Circular map for MR231437

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankle2
• Synonyms: 1110001J12Rik; AI661024; D5Ertd585e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231437 representing NM_001253814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGCAGCGGCTGGCTGTGGTGGAGTGGGCGGCCCTGGCCTGGGAGCTCCTGGGCGCCTCGGTGC
  TGTTCATCGCCGTGCGCTGGCTGGTCCGCCGGCTAGAGAAGCGGCCGCGGGACCTGAACCGTTGCGGGAC
  GCTCTCCTCTCCGCCCAGCGCGTCTGAAGCGGTGGCCGCACAGCCAGGTGAAGTGACAATGGATGCCATG
  ATGGCTCGACTGAAACTCTTGAATCCAGATGACCTTAGAAAAGAAGTTATGAAAGCTGGACTTAAATGTG
  GACCCATTACATCAACAACAAGATTTATTTTTGAGAAGAAATTAGCTCAGGCCTTACTGGAACAAGGTGG
  ATTGCTGACCTCTTCCCTTCCCAAGCCCAGCGCGGTTACTGCCATGGCATTTATCCAGGGCACCTCAAGG
  ACTCCTCCATCTGTGGACGGGAAACAAACTCAGCAGGCCTGTTTTTCTGAAGACAGAGATTTTGGTTACA
  GTGTGGGCTTGAACCCTCCAGAAGAGGAAGCGGTAGCATCCAGTGTCCACCCAGTACCCTTCAGTGCCTC
  TACCAGGAATGACAACCACAAGGCTGGAGTGACAGCTCCTAAGGAGCCACTTGTGTACTATGGAGTGTGT
  CCAGTATATGAGGATGGCCCTGTGAGACATGAGAGGATCCATGTTTACGAAGATAAAAAGGAAGCTTTAC
  AAGCTGCCAAGTTGATCAAAGGGTCCCGGTTTAAAGCTTTTCGAACAAGAGAAGATGCTGAGAAATTTGC
  TAGAGGGATATGTGATTATTTACCTTCTCCCAACAAAACTACGCCCTTACTGTCTCCTGTGAAGGCAGTG
  CCCCTCGGTGGCAGTGATGGGCTGAAAGCAGATGGCTTGTGCCTGGCTGAATCAGAAACAGTAAACAAAG
  AACGAGCAAACAGTTACAAAAACCCCCGTACCCAGGACCTCACGGCCAAGCTGCGGAAAGCTGTCGAAAA
  CGGGGAGGAACACACCTTTTCTGATCTTATCTGGAGTAATCCTCGATATCTAATTGGTTCTGGGGACAAC
  CCAACCATTGTACAAGAAGGATGTAGGTACAATGTCATGCATGTTGCTGCCAAAGAGAACCAGGCTTCCA
  TGTGCCAGCTGACATTAGAGACTCTGGAGAACCCTGAGTTTATGCGTCTGATGTACCCAGATGACAACAT
  GGACATGCTACAGAAGCGCATCCTCTACGTTGTTGACCTGTATCTTAATACTCCTGACAAAGTGGGCTTT
  GATACACCACTGCATTTTGCTTGTAAATTTGGAAATGTGGATGTAGTCAATGTGCTTTCTTCACACCCTT
  TGATTGTGAAAAACCGAAAGAATAAATATGGTAAAACACCTGAAGAAGTAATTTGTGAAAGAAGCCAAAA
  CAAATCTCCAGCACTGAAAGAAAGGATTAGAGAATACTTAATGGGTCACTACTATGTGCCACTCCTGAGA
  GCAGAAGACACTTCTCCTGTCATTGGGGAGCTGTGGTCCTCAGACCAGAAAGCTGAAGCCTCAAATACTG
  CCCACTGTAGAAGCAGCCCCAGAGATCCTGTGATGACACTGAGAGCTTTCGTGGGACCTCTGAGTCCATC
  CAAAGCAGAAGATTTTCGAAAGCTCTGGAAAACTCCACCTCGAAAGAAAGCAGGCTTCTTCCACAGCATC
  AGAAAGTCTGACCCAGAACGAGGCATTGAGAGAGTTGGAAGGGAGCTAGCTCATGAGCTGGGGTATCCCT
  GGGTTGAATACTGGGAATTTCTGGGATGTTTTGTTGATCTGTCTTCCCAGGAAGGCTTGCAAAGACTAGA
  AGAATACCTTATCCAGAAGGAGTTAAGCAAAAAGGCTCAACAAGAAATACGAGAAAATGAAGGCTGCCTT
  CAGGACAGAACCTCAGATTTTGGCAGTGGAAAGAAGTACAGCAACTCCATCTCCGTGGGAGCATTTCTAG
  ATGGGGATGATGACAGTAGTTTGGAAGAAATTAAAAACCAGCAAAATACAGTTCCAAGTCAGAGTCAGCC
  CACAACTGATAAGTTCCAAACCTCTAAAAGTGGTTCCCTTCCCTTGGGACAGAAGGTCGACCCTGGAGAA
  ACCTCAGTTGGAACTTACCCAGATAAAGGCAGAAATGGGTTTTGTCACCCTCTAAACCACAGGACTGCAG
  ATGGAAGGGGACTAGAGGCCACCAATGGGGAGGAGGCCCTTCCACCACCTGTTTCTGTTTTGACTCAAGA
  GTTGAATAAACTGAATTTGCAAAGCCTGGGAGACAGCCTTCATGAGACACCAGATAAAAACGGGAAACTA
  GAGGATGAGGTGCTTCCTTCAAGAAAGGGTGCAGCAGACAGTGACTTGCTGGCATCTCCTCCTGCCATAG
  CAAGCCTTGGAAAGAAGCAAGTCAGGACCAACACTGAGGTGTCAGAGGCAATGGCTGAAATGTCTTTGGG
  CCCTAAGAGCCCCCAACTTGGAGTTCAGGCTGGTCTGGAACCTATACTCTCATCTGCCACGGTAGATTCC
  ACCAAGAGGCTCTTCCTGTCTGGAGAGGAACCATCAAAACTAGATCGAGATGTTTTAGCAGCTCTTGAGT
  GTGCGAATATTGACCCTGGTCTGTACCCAGCAATCCACAGATGGAAAAGCACTGTCATGTGCTACTCGCC
  CTCAGACAGACAGAGTTGGCCAAGTCCTGCACTCAAAGGGAAGTTCACAACTGAGTTGGTGGATCTCGAT
  TGCTCTCATAGCTGCTCAGGACGATGCAGTCCAGCTGGTAGCAGCCCCAGTAAGCCTGGCCACACCTCCT
  CCTCTTCTGGCCTGCACAGCCCTGGACGCTACAGTCCAGCACATGGGAGACACTTCCAAAGAGTGGCACA
  CGTGGCCCGACTTGCAGCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231437 representing NM_001253814
  Red=Cloning site Green=Tags(s)
  
  MLWQRLAVVEWAALAWELLGASVLFIAVRWLVRRLEKRPRDLNRCGTLSSPPSASEAVAAQPGEVTMDAM
  MARLKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQALLEQGGLLTSSLPKPSAVTAMAFIQGTSR
  TPPSVDGKQTQQACFSEDRDFGYSVGLNPPEEEAVASSVHPVPFSASTRNDNHKAGVTAPKEPLVYYGVC
  PVYEDGPVRHERIHVYEDKKEALQAAKLIKGSRFKAFRTREDAEKFARGICDYLPSPNKTTPLLSPVKAV
  PLGGSDGLKADGLCLAESETVNKERANSYKNPRTQDLTAKLRKAVENGEEHTFSDLIWSNPRYLIGSGDN
  PTIVQEGCRYNVMHVAAKENQASMCQLTLETLENPEFMRLMYPDDNMDMLQKRILYVVDLYLNTPDKVGF
  DTPLHFACKFGNVDVVNVLSSHPLIVKNRKNKYGKTPEEVICERSQNKSPALKERIREYLMGHYYVPLLR
  AEDTSPVIGELWSSDQKAEASNTAHCRSSPRDPVMTLRAFVGPLSPSKAEDFRKLWKTPPRKKAGFFHSI
  RKSDPERGIERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLIQKELSKKAQQEIRENEGCL
  QDRTSDFGSGKKYSNSISVGAFLDGDDDSSLEEIKNQQNTVPSQSQPTTDKFQTSKSGSLPLGQKVDPGE
  TSVGTYPDKGRNGFCHPLNHRTADGRGLEATNGEEALPPPVSVLTQELNKLNLQSLGDSLHETPDKNGKL
  EDEVLPSRKGAADSDLLASPPAIASLGKKQVRTNTEVSEAMAEMSLGPKSPQLGVQAGLEPILSSATVDS
  TKRLFLSGEEPSKLDRDVLAALECANIDPGLYPAIHRWKSTVMCYSPSDRQSWPSPALKGKFTTELVDLD
  CSHSCSGRCSPAGSSPSKPGHTSSSSGLHSPGRYSPAHGRHFQRVAHVARLAAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253814
• ORF Size: 2892 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253814.1, NP_001240743.1
• RefSeq Size: 5259 bp
• RefSeq ORF: 2895 bp
• Locus ID: 71782
• UniProt ID: Q6P1H6
• Cytogenetics: 5 53.4 cM
• MW: 106.6 kDa
• Gene Summary: Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (By similarity).[UniProtKB/Swiss-Prot Function]