Hk3 (NM_001206392) Mouse Tagged ORF Clone
CAT#: MR231286
- TrueORF®
Hk3 (myc-DDK-tagged) - Mouse hexokinase 3 (Hk3), transcript variant 4
"NM_001206392" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hk3 |
Synonyms | HK-III; HK III |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231286 representing NM_001206392
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGGCACTGAAGGGACAGGACAGTCCCGCTCCTTCTGTCCGGATGCTGCCCACATACGTGAGGT CCACACCACACGGCACCGAGCAAGGGGATTTCCTGGTGCTGGAGCTGGGGGCCACAGGAGCATCACTACG TGTGTTGTGGGTAACACTGACAGGCACCAAAGAATGCCGGGTGGAGCCCAGGAGCCGGGAGTTTGTGATT CCTCAAGAGGTGATCCTAGGTGCTGGCCAGCAGCTCTTTGACTTTGCTGCCCGATGCCTCTCTGAATTCC TGGATGCATACCCCGTGGAGAATCAGGGTCTGAAGCTTGGGTTCAATTTCTCTTTTCCTTGTCACCAGAC AGGCTTGGACAGGAGCACCCTCATTTCCTGGACAAAAGGTTTTAGGTGCAGCGGCGTGGAAGGCCAGGAT GTGGTCCAGTTGCTAAGAGATGCCATTCAGAGGCAGGGGACCTACAGGATTGATGTGGTAGCCATGGTGA ATGACACGGTGGGCACCATGATGGGCTGTGAGCTGGGCACCAGGCCGTGTGAAGTCGGGCTGATTGTAGA CACCGGTACCAACGCGTGCTACATGGAGGAGGCGCGGCACGTGGCCGCTCTGGATGAGGACCGCGGCCGC ACCTGTGTCAGCATCGAGTGGGGCTCCTTCTATGACGAAGATGCTCTAGGGCCAGTACTGACCACCTTCG ACTCTGCCCTGGACCGCGAGTCCCTGACTCCTGGTGCTCAAAGGTTTGAGAAGATGATTGGAGGCCTTTA CCTGGGTGAGCTGGTGAGGCTGGTTCTGGTCCACTTGACCCAGCACGGGGTCCTCTTTGATGGCTGCGCC TCTCCTGCGTTGCTGAGTCAAGGCTGTATCCTCCTGGACCACGTGGCTGAGATGGAGGACACCGCCACTG GGACAGCCCGCGTCCACACCATCCTGCAGGACTTGGGTCTGAGCCCTCGGGCCTCAGATGCTGAACTGGT GCAATATGTGTGCGTGGCTGTGTGCACACGGGCTGCCCAGCTCTGTGCGGCTGCCCTGGCTGCAGTCCTC TCCCGCCTCCAGCACAGCAGGGAGCAGCAGACATTGCAAGTGGCTGTGGCCACTGGAGGGCGAGTGTTTG AACGACACCCCAGGTTCCTCCGAATCCTAAAGGAGACGGTAACACTCCTGGCCCCAAACTGTGATGTCTC CTTCATCCCCTCTGTGGATGGTGGTGGCCGGGGTGTGGCAATGGTGACAGCTGTGGCTGCCCGCCTGGCT GCTCACAGGCGCATCCTGGAAGAGACCCTGGCACCATTTCAGCTGACCTTGGAGCAGATGACAGTGGTGC AGGCACAAATGCGGGAAGCCATGATCAGGGGGCTTCAAGGAGAGGCCTCCTCCCTCCGCATGCTGCCCAC TTACGTCCGAGCGACGCCCGACGGCAGCGAGCGAGGTGATTTCCTGGCCTTGGACCTAGGGGGCACCAAC TTCCGGGTCCTGTTGGTACGAGTGGCGGAGGGCAGTGTGCAGATCATCAACCAGGTCTACTCCATTCCTG AGTGTAGAGCCCAGGGCTCTGGACAGAAGCTCTTTGACCATATTGTGGACTGCATCGTGGACTTCCAGAA GAGGCAGGGCCTTAGTGGACAGAGCCTACCCCTGGGTTTCACCTTCTCTTTCCCATGCAAGCAGCTTGGC CTGGACCAGGGCATCCTCCTGAATTGGACTAAGGGGTTCAATGCATCAGGCTGTGAGGGCCAGGATGTTG TGTATCTATTACGGGAAGCCATTAGGCGCAGACAGGCAGTGGAGCTGAATGTGGTTGCCATTGTCAATGA CACGGTGGGGACCATGATGTCCTGTGGCTATGATGATCCCCGTTGTGAGATGGGCCTCATTGTCGGAACC GGCACCAACGCCTGTTACATGGAAGAGCTCCGCAATGTGGCAAGTGTGCCTGGGGACTCGGGCCTCATGT GCATCAACATGGAGTGGGGTGCCTTTGGGGATGATGGCTCACTGGGCACACTCAGCACCCGCTTTGACAC CAGTGTGGACCAGGCATCCATCAATCCAGGCAAACAGAGGTTTGAGAAAATGATCAGCGGTATGTACTTG GGGGAGATCGTCCGCCATATCCTCCTGCACTTAACCAATCTCGGAGTTCTCTTCCGAGGCCAGAAGACTC AATGCCTTCAGGCCAGGGACATCTTCAAGACTAAGTTCCTCTCTGAGATCGAAAGTGATAGCCTGGCCCT GCGTCAGGTCCGAGCCATCCTGGAAGATCTGGGGCTGACTCTGACATCTGATGACGCCTTGATGGTCCTG GAAGTGTGCCAGGCTGTGTCTCGCAGGGCTGCCCAACTCTGTGGGGCAGGTGTGGCTGCCGTGGTGGAGA AGATTCGGGAGAACCGGGGCCTGCAGGAGCTGACAGTGTCTGTGGGAGTAGATGGGACACTCTACAAGCT ACATCCTCACTTCTCCAAGCTGGTGTCGGCAACAGTCCGGAAGCTAGCCCCTCAGTGCACCGTCACCTTT TTGCAGTCGGAGGATGGGTCTGGCAAAGGCGCAGCTTTGGTTACTGCTGTTGCTTGTCGCCTTACCCAGA TGGCCCACGTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR231286 representing NM_001206392
Red=Cloning site Green=Tags(s) MEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVI PQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQD VVQLLRDAIQRQGTYRIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGR TCVSIEWGSFYDEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCA SPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTRAAQLCAAALAAVL SRLQHSREQQTLQVAVATGGRVFERHPRFLRILKETVTLLAPNCDVSFIPSVDGGGRGVAMVTAVAARLA AHRRILEETLAPFQLTLEQMTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTN FRVLLVRVAEGSVQIINQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLG LDQGILLNWTKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGT GTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASINPGKQRFEKMISGMYL GEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVL EVCQAVSRRAAQLCGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTF LQSEDGSGKGAALVTAVACRLTQMAHV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001206392 |
ORF Size | 2601 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001206392.1, NP_001193321.1 |
RefSeq Size | 3099 bp |
RefSeq ORF | 2604 bp |
Locus ID | 212032 |
Cytogenetics | 13 B1 |
MW | 94.7 kDa |
Gene Summary | Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229075 | Hk3 (untagged) - Mouse hexokinase 3 (Hk3), transcript variant 4 |
USD 874.00 |
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