Lrrc4c (NM_001289742) Mouse Tagged ORF Clone

CAT#: MR230761

Lrrc4c (myc-DDK-tagged) - Mouse leucine rich repeat containing 4C (Lrrc4c), transcript variant 1

Product Images

Circular map for MR230761

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc4c
• Synonyms: 6430556C10Rik; NGL-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230761 representing NM_001289742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAACAAGATGACCTTACATCCACAGCAGATAATGATAGGTCCTAGGTTTAACAGGGCCCTATTTG
  ACCCCCTGCTTGTGGTGCTGTTGGCTCTTCAACTTCTTGTGGTGGCTGGTTTGGTTCGTGCTCAAACCTG
  CCCTTCAGTGTGCTCTTGTAGCAACCAGTTCAGCAAGGTGATTTGTGTTCGGAAAAACCTTCGTGAAGTT
  CCGGATGGCATCTCCACCAACACAAGGCTGCTGAACCTCCATGAGAACCAAATCCAGATCATTAAAGTGA
  ACAGCTTCAAGCATTTGAGGCACTTAGAAATCCTCCAGTTGAGCAGGAACCATATTCGAACCATTGAAAT
  CGGGGCCTTCAATGGTCTGGCCAACCTCAACACCCTGGAACTCTTTGACAATCGTCTTACTACCATACCG
  AATGGAGCTTTTGTATATTTGTCTAAACTGAAGGAGCTCTGGTTGAGGAACAACCCTATTGAAAGCATCC
  CTTCCTATGCTTTTAACAGAATCCCTTCTTTGCGCCGCCTGGACTTAGGGGAATTGAAAAGGCTTTCATA
  CATCTCAGAAGGTGCCTTTGAAGGTCTGTCCAACTTGAGGTATTTGAACCTTGCCATGTGCAACCTTCGG
  GAAATCCCTAACCTCACACCACTCATCAAACTTGACGAGCTAGATCTTTCTGGGAACCATTTGTCTGCAA
  TCAGGCCTGGCTCTTTTCAGGGGTTGATGCACCTTCAAAAACTGTGGATGATACAGTCTCAGATTCAAGT
  GATTGAACGGAATGCCTTTGATAACCTTCAGTCACTAGTGGAGATAAACTTGGCACACAACAATCTAACA
  TTATTGCCTCATGACCTCTTCACACCCTTGCATCATCTAGAGAGGATACACCTCCATCACAACCCGTGGA
  ACTGTAACTGTGATATCCTGTGGCTCAGCTGGTGGATAAGAGACATGGCCCCCTCCAACACAGCTTGCTG
  TGCCAGGTGTAACACTCCCCCCAACCTGAAAGGGAGGTACATCGGAGAGCTGGACCAGAATTACTTTACA
  TGCTATGCTCCCGTAATTGTGGAGCCCCCTGCAGACCTCAATGTCACTGAAGGCATGGCAGCTGAGCTGA
  AATGTCGGGCTTCTACGTCCCTGACTTCCGTATCTTGGATTACTCCAAATGGAACAGTCATGACCCACGG
  GGCATACAAAGTGCGGATAGCTGTGCTCAGCGACGGTACTTTAAATTTCACAAATGTAACTGTGCAAGAC
  ACAGGCATGTACACATGTATGGTGAGTAATTCTGTTGGCAACACCACTGCTTCTGCCACCTTGAATGTTA
  CTGCGGCAACCACTACTCCGTTCTCGTACTTTTCAACTGTAACAGTAGAGACTATGGAACCTTCTCAGGA
  TGAGGCACGGACCACAGATAACAATGTGGGCCCCACTCCAGTGATCGATTGGGAGACCACCAATGTAACC
  ACATCTCTTACGCCACAGAGCACAAGGTCGACAGAAAAAACATTCACCATCCCAGTAACTGACATCAACA
  GCGGAATCCCAGGAATTGATGAGGTCATGAAAACAACTAAAATCATTATTGGGTGTTTTGTGGCCATCAC
  ACTCATGGCTGCCGTGATGCTGGTCATTTTCTACAAGATGAGGAAACAGCACCATCGGCAAAACCACCAT
  GCTCCAACAAGGACTGTTGAAATCATTAACGTGGATGATGAGATCACTGGGGACACGCCCATGGAAAGCC
  ACCTGCCCATGCCTGCGATTGAGCATGAGCACCTAAACCACTATAACTCTTACAAATCTCCCTTCAACCA
  CACAACAACAGTAAACACAATAAATTCAATACACAGTTCAGTGCATGAACCGTTATTGATCCGAATGAAC
  TCTAAAGACAATGTACAAGAGACTCAGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230761 representing NM_001289742
  Red=Cloning site Green=Tags(s)
  
  MLNKMTLHPQQIMIGPRFNRALFDPLLVVLLALQLLVVAGLVRAQTCPSVCSCSNQFSKVICVRKNLREV
  PDGISTNTRLLNLHENQIQIIKVNSFKHLRHLEILQLSRNHIRTIEIGAFNGLANLNTLELFDNRLTTIP
  NGAFVYLSKLKELWLRNNPIESIPSYAFNRIPSLRRLDLGELKRLSYISEGAFEGLSNLRYLNLAMCNLR
  EIPNLTPLIKLDELDLSGNHLSAIRPGSFQGLMHLQKLWMIQSQIQVIERNAFDNLQSLVEINLAHNNLT
  LLPHDLFTPLHHLERIHLHHNPWNCNCDILWLSWWIRDMAPSNTACCARCNTPPNLKGRYIGELDQNYFT
  CYAPVIVEPPADLNVTEGMAAELKCRASTSLTSVSWITPNGTVMTHGAYKVRIAVLSDGTLNFTNVTVQD
  TGMYTCMVSNSVGNTTASATLNVTAATTTPFSYFSTVTVETMEPSQDEARTTDNNVGPTPVIDWETTNVT
  TSLTPQSTRSTEKTFTIPVTDINSGIPGIDEVMKTTKIIIGCFVAITLMAAVMLVIFYKMRKQHHRQNHH
  APTRTVEIINVDDEITGDTPMESHLPMPAIEHEHLNHYNSYKSPFNHTTTVNTINSIHSSVHEPLLIRMN
  SKDNVQETQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289742
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289742.1, NP_001276671.1
• RefSeq Size: 3860 bp
• RefSeq ORF: 1923 bp
• Locus ID: 241568
• UniProt ID: Q8C031
• Cytogenetics: 2 E1
• MW: 72.4 kDa
• Gene Summary: May promote neurite outgrowth of developing thalamic neurons.[UniProtKB/Swiss-Prot Function]