Epn1 (NM_001252454) Mouse Tagged ORF Clone
CAT#: MR230560
- TrueORF®
Epn1 (myc-DDK-tagged) - Mouse epsin 1 (Epn1), transcript variant 2
"NM_001252454" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Epn1 |
Synonyms | AA269831; Ibp1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230560 representing NM_001252454
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACCTCATCGCTGCGGCGTCAGATGAAGAATATCGTCCACAACTACTCAGAGGCTGAGATCAAGG TCCGCGAGGCCACAAGCAATGATCCCTGGGGCCCATCCAGCTCCCTCATGTCAGAGATTGCTGACCTCAC CTACAATGTTGTGGCCTTCTCCGAGATCATGAGCATGATCTGGAAGCGGCTCAATGACCACGGCAAGAAC TGGAGGCACGTCTACAAGGCCATGACGTTGATGGAGTACCTCATCAAGACTGGCTCTGAGCGTGTATCAC AACAGTGCAAAGAGAACATGTATGCTGTGCAGACGCTGAAGGACTTCCAGTATGTGGACCGTGATGGCAA GGACCAGGGTGTGAATGTGCGGGAGAAGGCCAAGCAGCTGGTGGCTTTGCTGCGTGATGAGGATCGGCTC CGGGAGGAGCGCGCCCACGCGCTCAAGACCAAGGAGAAGCTGGCACAGACTGCCACGGCCTCCTCAGCAG CCGTGGGCTCTGGACCACCTCCCGAGGCGGAGCAGGCATGGCCACAGAGCAGCGGGGAGGAGGAGTTGCA GCTCCAGCTGGCCCTGGCTATGAGCAAGGAGGAGGCCGACCAGCCCCCGTCCTGCGGCCCTGAGGATGAC GTCCAGCTCCAGCTGGCCCTTAGTTTGAGCCGAGAGGAGCACGATAAGGAAGAGCGGATCCGTCGTGGGG ATGACCTGAGGCTGCAGATGGCAATAGAGGAGAGCAAGAGGGAGACTGGGGGCAAGGAGGAGTCATCTCT CATGGATCTTGCTGATGTCTTCACAACCCCAGCGCCTCCACAGGCCTCAGACCCTTGGGGGGGCCCGGCA TCTGTGCCTACTGCTGTCCCTGTGGCTGCTGCCGCTTCAGACCCTTGGGGGGGACCTGCTGTCCCTCCAG CTGCTGATCCTTGGGGTGGTGCAGCCCCCACACCAGCCTCAGGGGATCCCTGGAGGCCTGCAGCCCCTAC AGGGCCCTCTGTTGATCCTTGGGGTGGGACCCCAGCTCCTGCAGCTGGAGAGGGGCCCACCCCTGACCCG TGGGGAAGCTCTGATGGTGGGGCTCCTGTCAGTGGGCCCCCATCTTCTGACCCCTGGGCACCTGCCCCGG CTTTCTCAGACCCCTGGGGAGGCTCACCTGCAAAGCCCAGCAGCAACGGCACTGCAGTTGGGGGCTTTGA CACAGAGCCTGATGAGTTCTCAGACTTCGACCGACTCCGCACTGCCCTGCCAACCTCTGGGAGCAGCACG GGGGAGTTGGAGTTGCTCGCAGGAGAGGTGCCTGCTCGCAGCCCTGGGGCATTCGACATGAGTGGGGTTG GGGGATCTCTGGCTGAGTCTGTGGGGAGTCCCCCACCTGCTGCAACCCCAACTCCCACCCCCCCAACACG GAAGACTCCGGAGTCATTCCTAGGCCCTAATGCAGCCCTTGTGGATCTGGACTCGCTGGTGAGCCGACCA GGCCCCACACCACCAGGATCCAAGGCTTCCAACCCATTCCTTCCAAGCGGAGCTCCGCCCACAGGCCCCT CTGTCACCAATCCTTTCCAGCCAGCACCCCCTGCAACGCTCACCCTGAACCAGCTCCGTCTCAGTCCTGT GCCCCCAGTTCCTGGAGCGCCACCCACCTATATCTCTCCTCTTGGTGGAGGCCCTGGCCTGCCCCCCATG ATGCCCCCAGGTCCCCCAGCCCCCAACACTAACCCCTTCCTCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230560 representing NM_001252454
Red=Cloning site Green=Tags(s) MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKN WRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRL REERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQPPSCGPEDD VQLQLALSLSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTTPAPPQASDPWGGPA SVPTAVPVAAAASDPWGGPAVPPAADPWGGAAPTPASGDPWRPAAPTGPSVDPWGGTPAPAAGEGPTPDP WGSSDGGAPVSGPPSSDPWAPAPAFSDPWGGSPAKPSSNGTAVGGFDTEPDEFSDFDRLRTALPTSGSST GELELLAGEVPARSPGAFDMSGVGGSLAESVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRP GPTPPGSKASNPFLPSGAPPTGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPM MPPGPPAPNTNPFLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001252454 |
ORF Size | 1725 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001252454.1, NP_001239383.1 |
RefSeq Size | 2411 bp |
RefSeq ORF | 1728 bp |
Locus ID | 13854 |
UniProt ID | Q80VP1 |
Cytogenetics | 7 A1 |
MW | 60.2 kDa |
Gene Summary | Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228349 | Epn1 (untagged) - Mouse epsin 1 (Epn1), transcript variant 2 |
USD 590.00 |
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