Abcg8 (NM_001286005) Mouse Tagged ORF Clone
CAT#: MR230461
- TrueORF®
Abcg8 (myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8), transcript variant 2
"NM_001286005" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Abcg8 |
Synonyms | 1300003C16Rik; AI114946; sterolin-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230461 representing NM_001286005
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCAGGACAAATTTGGATAAATGGGCAACCCAGTACGCCTCAGCTGGTGAGGAAGTGCGTTGCGC ATGTGCGGCAGCATGACCAACTGCTGCCCAACCTGACCGTCAGAGAGACCCTGGCTTTCATTGCCCAGAT GCGCCTGCCCAGGACCTTCTCCCAGGCCCAGCGTGACAAACGGGTGGAAGACGTAATCGCCGAGCTGCGG CTGCGGCAGTGCGCCAACACCAGAGTGGGCAACACGTATGTACGTGGGGTGTCCGGGGGTGAGCGCCGAC GAGTGAGCATTGGGGTGCAGCTCCTGTGGAACCCAGGAATCCTCATTCTGGATGAACCCACTTCTGGCCT CGACAGCTTCACAGCCCACAATCTGGTGACAACCTTGTCCCGCCTGGCCAAGGGCAACAGGCTGGTGCTC ATCTCCCTCCACCAGCCTCGCTCTGACATCTTCAGGCTATTTGACCTGGTCCTTCTGATGACATCTGGCA CCCCTATCTACCTGGGGGCGGCGCAGCAAATGGTGCAGTACTTCACATCCATTGGCCACCCTTGTCCTCG CTATAGCAACCCTGCGGACTTCTACGTGGACTTGACCAGCATCGACAGACGCAGCAAAGAACGGGAGGTG GCCACCGTGGAGAAGGCACAGTCTCTTGCAGCCCTGTTCCTAGAAAAAGTACAAGGCTTTGATGACTTTC TGTGGAAAGCTGAGGCAAAGGAACTCAACACAAGCACCCACACAGTCAGCCTGACCCTCACACAGGACAC TGACTGTGGGACTGCTGTTGAGCTGCCCGGGATGATAGAGCAGTTTTCCACCCTGATCCGTCGTCAGATT TCCAATGACTTCCGGGACCTGCCCACGCTGCTCATTCATGGGTCGGAAGCCTGCCTGATGTCCCTCATCA TTGGCTTCCTTTACTACGGCCATGGGGCCAAGCAGCTCTCCTTCATGGACACAGCAGCCCTCCTCTTCAT GATAGGGGCGCTCATTCCTTTCAATGTCATCCTGGATGTCGTCTCCAAATGTCACTCGGAGAGGTCAATG CTGTACTATGAGCTGGAAGACGGGCTGTACACTGCTGGTCCTTATTTCTTTGCCAAGATCCTAGGAGAAT TGCCGGAGCACTGTGCCTACGTCATCATCTACGCGATGCCCATCTACTGGCTGACAAACCTGCGGCCCGT GCCTGAGCTCTTCCTTCTACACTTCCTGCTCGTGTGGTTGGTGGTCTTCTGCTGCAGGACCATGGCCCTG GCTGCCTCTGCCATGCTGCCCACCTTCCACATGTCCTCCTTCTTCTGCAATGCCCTCTACAACTCCTTCT ACCTTACTGCCGGCTTCATGATAAACTTGGACAACCTGTGGATAGTGCCTGCATGGATCTCCAAGCTGTC GTTCCTCCGGTGGTGCTTCTCGGGGCTGATGCAGATTCAATTTAATGGACACCTTTACACCACACAAATC GGCAACTTCACCTTCTCCATCCTCGGAGACACGATGATCAGTGCCATGGACCTGAACTCGCATCCACTCT ATGCGATCTACCTCATTGTCATCGGCATCAGCTACGGCTTCCTGTTCCTGTACTATCTATCCTTGAAGCT CATCAAACAGAAGTCAATTCAAGACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230461 representing NM_001286005
Red=Cloning site Green=Tags(s) MKSGQIWINGQPSTPQLVRKCVAHVRQHDQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELR LRQCANTRVGNTYVRGVSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVTTLSRLAKGNRLVL ISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQQMVQYFTSIGHPCPRYSNPADFYVDLTSIDRRSKEREV ATVEKAQSLAALFLEKVQGFDDFLWKAEAKELNTSTHTVSLTLTQDTDCGTAVELPGMIEQFSTLIRRQI SNDFRDLPTLLIHGSEACLMSLIIGFLYYGHGAKQLSFMDTAALLFMIGALIPFNVILDVVSKCHSERSM LYYELEDGLYTAGPYFFAKILGELPEHCAYVIIYAMPIYWLTNLRPVPELFLLHFLLVWLVVFCCRTMAL AASAMLPTFHMSSFFCNALYNSFYLTAGFMINLDNLWIVPAWISKLSFLRWCFSGLMQIQFNGHLYTTQI GNFTFSILGDTMISAMDLNSHPLYAIYLIVIGISYGFLFLYYLSLKLIKQKSIQDW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001286005 |
ORF Size | 1638 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286005.1, NP_001272934.1 |
RefSeq Size | 3432 bp |
RefSeq ORF | 1641 bp |
Locus ID | 67470 |
UniProt ID | Q9DBM0 |
Cytogenetics | 17 55.02 cM |
MW | 62.4 kDa |
Gene Summary | ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane (PubMed:16352607, PubMed:16867993, PubMed:18402465). Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed:12444248, PubMed:14504269, PubMed:14657202, PubMed:25378657). Plays an important role in preventing the accumulation of dietary plant sterols in the body (PubMed:12444248, PubMed:14657202). Required for normal sterol homeostasis (PubMed:12444248, PubMed:14657202). The heterodimer with ABCG5 has ATPase activity (PubMed:16352607, PubMed:16867993).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228250 | Abcg8 (untagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8), transcript variant 2 |
USD 560.00 |
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