Pnkp (NM_001290764) Mouse Tagged ORF Clone

CAT#: MR230359

Pnkp (myc-DDK-tagged) - Mouse polynucleotide kinase 3'- phosphatase (Pnkp), transcript variant 2

Product Images

Circular map for MR230359

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pnkp
• Synonyms: 1810009G08Rik; PNK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230359 representing NM_001290764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAGCTTGGATCCCGGGGCCGCCTGTGGCTGCAGAGCCCCACCGGTGGGCCGCCCCCTATATTCC
  TGCCGTCGGATGGCCAAGCCCTGGTCCTCGGTCGGGGACCCCTGACCCAAGTGACGGACCGGAAGTGCTC
  CCGAAACCAAGTGGAGCTGATTGCAGACCCTGAAAGCCGGACAGTGGCAGTGAAACAGCTGGGTGTTAAC
  CCGTCAACTGTTGGGGTCCATGAGTTGAAGCCAGGACTGTCTGGCTCTCTAAGCTTGGGGGATGTCCTTT
  ATCTGGTTAATGGCCTATATCCCCTGACCCTGCGTTGGGAAGAGCTCAGCACATCAGGATCCCAGCCAGA
  TGCTCCACCAGACACTCCTGGGGATCCGGAGGAGGGAGAGGATACTGAGCCTCAGAAAAAGCGAGTTCGG
  AAATCCTCTCTTGGCTGGGAGAGCTTAAAGAAGTTACTGGTGTTCACAGCGTCTGGGGTGAAGCCCCAGG
  GCAAGGTGGCTGCCTTTGACCTAGATGGGACCCTCATCACCACCCGCTCTGGAAAGGTCTTCCCCACTAG
  CCCTAGTGACTGGAGGATTCTGTACCCAGAGATTCCAAAGAAGCTCCAGGAGCTGGCTGCTGAAGGCTAC
  AAGTTGGTAATCTTCACCAACCAAATGGGCATCGGGCGAGGCAAGCTGCCAGCAGAGGTGTTCAAGGGCA
  AGGTGGAAGCTGTATTGGAGAAGTTAGGGGTCCCATTTCAGGTGCTGGTGGCAACACATGCAGGTCTAAA
  CCGAAAGCCAGTGAGTGGCATGTGGGACCATCTGCAGGAGCAGGCCAACGAGGGCATCCCCATCTCGGTA
  GAGGACAGTGTCTTCGTGGGAGATGCAGCAGGGCGCCTAGCCAACTGGGCACCGGGGAGGAAAAAAAAGG
  ATTTCTCTTGTGCAGACCGCCTGTTCGCCCTTAACGTCGGCCTGCCCTTTGCCACTCCGGAAGAGTTTTT
  CCTTAAGTGGCCGGCTGCCCGCTTTGAGCTTCCAGCTTTCGACCCGAGGACTATCTCCAGTGCTGGGCCC
  CTCTACCTTCCAGAGTCCAGCTCTCTCCTGAGCCCCAACCCAGAGGTGGTGGTCGCAGTAGGATTCCCTG
  GGGCCGGCAAGTCCACTTTCATCCAGGAGCACCTAGTGTCAGCTGGCTACGTCCATGTGAACAGGGACAC
  GCTGGGCTCGTGGCAGCGCTGTGTGAGCTCATGCCAGGCGGCACTGAGGCAGGGGAAGCGAGTCGTGATT
  GACAACACCAACCCAGATGTCCCAAGTCGGGCCAGGTACATCCAATGTGCCAAAGATGCAGGTGTGCCCT
  GCCGCTGTTTCAACTTCTGTGCTACAATAGAGCAGGCACGCCACAACAACAGGTTCCGTGAGATGACCGA
  CCCTTCGCATGCACCTGTGTCGGACATGGTCATGTTTAGCTACAGGAAGCAGTTTGAGCCGCCCACACTG
  GCTGAAGGCTTCCTGGAGATCCTTGAGATTCCATTCCGGTTACAGGAGCATCTGGACCCGGCACTGCAGC
  GACTGTACCGCCAGTTTTCCGAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230359 representing NM_001290764
  Red=Cloning site Green=Tags(s)
  
  MSQLGSRGRLWLQSPTGGPPPIFLPSDGQALVLGRGPLTQVTDRKCSRNQVELIADPESRTVAVKQLGVN
  PSTVGVHELKPGLSGSLSLGDVLYLVNGLYPLTLRWEELSTSGSQPDAPPDTPGDPEEGEDTEPQKKRVR
  KSSLGWESLKKLLVFTASGVKPQGKVAAFDLDGTLITTRSGKVFPTSPSDWRILYPEIPKKLQELAAEGY
  KLVIFTNQMGIGRGKLPAEVFKGKVEAVLEKLGVPFQVLVATHAGLNRKPVSGMWDHLQEQANEGIPISV
  EDSVFVGDAAGRLANWAPGRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFDPRTISSAGP
  LYLPESSSLLSPNPEVVVAVGFPGAGKSTFIQEHLVSAGYVHVNRDTLGSWQRCVSSCQAALRQGKRVVI
  DNTNPDVPSRARYIQCAKDAGVPCRCFNFCATIEQARHNNRFREMTDPSHAPVSDMVMFSYRKQFEPPTL
  AEGFLEILEIPFRLQEHLDPALQRLYRQFSEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290764
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290764.1, NP_001277693.1
• RefSeq Size: 2042 bp
• RefSeq ORF: 1569 bp
• Locus ID: 59047
• Cytogenetics: 7 B3
• MW: 57.2 kDa