Agbl4 (NM_001284190) Mouse Tagged ORF Clone
CAT#: MR230224
- TrueORF®
Agbl4 (myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4), transcript variant 3
"NM_001284190" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Agbl4 |
Synonyms | 4930578N11Rik; 4931433A01Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230224 representing NM_001284190
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCGGAGCCAGACAGCGCCAGAGGCAGGCAATGATACAGGAAATGAGGATGCCATTGGAGGGA ATGTGAACAAATACATAGTGCTTCCAAATGGATACTCTGGACAGCCCAAGAAAGGACATCTTACCTTTGA TGCTTGCTTTGAAAGTGGTAACCTCGGCCGGGTTGAGCAAGTCTCTGATTTTGAGTATGATCTGTTCATT AGGCCGGACACCTGTAATCCACGCTTCCGAGTCTGGTTCAACTTTACTGTTGAAAATGTGAAAGAATTGC AGAGGGTAATTTTCAACATTGTTAACTTCAGTAAAACCAAGAGTCTTTACCGAGATGGGATGGCACCAAT GGTGAAATCTACCAGCAGACCAAAATGGCAAAGACTACCACCAAAAAACGTTTATTACTATCGCTGCCCA GACCACAGGAAGAACTATGTGATGTCCTTTGCATTCTGTTTTGACCGAGAAGATGATATCTACCAGTTTG CTTACTGCTACCCTTATACATATACTCGCTTCCAGCATTACCTCGACAGCTTACAAAAGAAAAACATGGA TTATTTCTTCCGGGAACAGCTTGGACAGAGTGTGCAACAGCGGCAGCTTGACCTCCTGACGATAACCAGC CCGGAGAATCTCCGTGAAGGGTCAGAAAAGAAGGTGATATTCATCACAGGGCGAGTCCACCCAGGGGAAA CGCCATCTTCATTTGTGTGCCAAGGAATCATCGACTTCCTTGTAAGTCAGCATCCAATTGCCCGTGTCCT ACGAGAACATTTAGTCTTCAAGATTGCTCCAATGCTCAACCCTGATGGAGTTTACCTGGGCAACTACAGG TGTTCCCTGATGGGGTTTGACCTGAATCGTCACTGGCTGGATCCCTCTCCATGGGCCCATCCCACCCTGC ATGGAGTGAAACAGCTTATTATCAAGATGTACAATGACCCAAAAACAAGCCTGGAGTTCTATATTGACAT CCACGCTCACTCCACCATGATGAATGGCTTCATGTACGGCAATATCTTTGAGGATGAGGAACGGTTCCAA AGGCAGTCCATTTTTCCAAAACTCCTTTGCCAGAATGCCGAGGACTTCTCCTATACTAGCACATCCTTCA ACAGGGATGCTGTGAAAGCAGGAACTGGCCGGCGCTTCCTGGGCGGGCTTCTGGACCACTCATCATACTG CTATACCCTAGAGGTTTCCTTCTACAGCTACATCATTGGGGGTACTACAGCTGCAGTGCCCTACACTGAA GAAGCCTATATGAAACTGGGACGGAACGTGGCAAGAACATTTCTAGATTATTACCGGCTGAACTCCCTGG TTGAGAAGATAGCAGTCCCCATGCCAAGACTTCGGAAAGAAAAAGCCCCTCCTTGCAAGCACCCACCCCC AAGGGGCTCAACCAGCAACGTCTCCATTGGCAAAGGGGACAAGAAGAACTCACTGAACCACAAAGACCCA TCAACCCCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230224 representing NM_001284190
Red=Cloning site Green=Tags(s) MAERSQTAPEAGNDTGNEDAIGGNVNKYIVLPNGYSGQPKKGHLTFDACFESGNLGRVEQVSDFEYDLFI RPDTCNPRFRVWFNFTVENVKELQRVIFNIVNFSKTKSLYRDGMAPMVKSTSRPKWQRLPPKNVYYYRCP DHRKNYVMSFAFCFDREDDIYQFAYCYPYTYTRFQHYLDSLQKKNMDYFFREQLGQSVQQRQLDLLTITS PENLREGSEKKVIFITGRVHPGETPSSFVCQGIIDFLVSQHPIARVLREHLVFKIAPMLNPDGVYLGNYR CSLMGFDLNRHWLDPSPWAHPTLHGVKQLIIKMYNDPKTSLEFYIDIHAHSTMMNGFMYGNIFEDEERFQ RQSIFPKLLCQNAEDFSYTSTSFNRDAVKAGTGRRFLGGLLDHSSYCYTLEVSFYSYIIGGTTAAVPYTE EAYMKLGRNVARTFLDYYRLNSLVEKIAVPMPRLRKEKAPPCKHPPPRGSTSNVSIGKGDKKNSLNHKDP STPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001284190 |
ORF Size | 1482 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001284190.2 |
RefSeq Size | 1659 bp |
RefSeq ORF | 1485 bp |
Locus ID | 78933 |
Cytogenetics | 4 C7-D1 |
MW | 57.5 kDa |
Gene Summary | Metallocarboxypeptidase that mediates deglutamylation of target proteins (PubMed:17244818, PubMed:21074048, PubMed:25103237, PubMed:26829768). Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins (PubMed:17244818). Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK (PubMed:21074048). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768). Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 (PubMed:25103237).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228013 | Agbl4 (untagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4), transcript variant 3 |
USD 503.00 |
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