Trim11 (NM_001290988) Mouse Tagged ORF Clone

CAT#: MR230145

Trim11 (myc-DDK-tagged) - Mouse tripartite motif-containing 11 (Trim11), transcript variant 1

Product Images

Circular map for MR230145

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230145 representing NM_001290988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCCAGACTTGTCCACCAACCTCCAGGAGGAGGCCACCTGCGCCATCTGCCTGGATTACTTCA
  CCGACCCGGTGATGACCGACTGCGGCCACAACTTCTGCCGCGAGTGCATCCGACGTTGCTGGGGCCAGCC
  CGAGGGCCCATACGCGTGCCCCGAGTGTCGCGAGCTGTCGGCGCAGAGGAACCTACGGCCCAACCGCCCG
  CTCGCCAAAATGGCAGAAATGGCACGACGCCTGCACCCGCCTTCTCCGGTCCCGCAGGGCGTCTGTGCGG
  CGCACCGCGAGCCGCTGACCACCTTCTGCGGCGACGACCTCAGCCTGCTGTGCCCCATCTGCGAGCGTTC
  GGAGCACTGGACTCACCGGGTGAGACCGCTGCAGGAGGCGGCGGACGACCTCAAGGGGAGGCTGGAGAAG
  TCACTGGAGCACCTACGGAAACAAATGGAGGACGCAATGCTGTTCCAAGCCCAGGCTGAGGAAACCTGTG
  CCTTGTGGCAGAAGATGGTGGAGAGCCAGCGGCAGAATGTGCTGGGCGAGTTTGAGAGGCTGCGCCGCCT
  GTTGGCAGAGGAGGAGCAACAGCTGCTGCAGAAGCTGGAGGAGGAAGAGCTGGAGGTTCTGCCGCGCCTG
  CGTGAAGGCGCTGCGAGGCTTGGCCAGCAAAGCACGCAGCTGGCAGCCCTCATCTCCGAGCTTGAGAGCC
  GCTGCCAGCTGCCAGCCCTGGGTTTACTGCAGCTCTGCATTGAGTGCTGCGCCCTGGAGAGAGAAGCGTC
  TATAGCCAAGGACATTAAGGACGCCCTGTGCAGGGTGCAGGATGTGAAGCTGCAGCCTCCAGCAGTGGTG
  CCCATGGAGCTGAGGACCGTGTGCCGGGTCCCAGGGCTGGTGGAGACCCTGCGGAGGTTCCGAGGGGACA
  TAACCCTGGACCCAGACACCGCCAACCCTGAGCTGGTCTTATCTGAGGACCGGAGGAGTGTGCAGCGTGG
  TGAACAGCGGCAGGCCCTGCCTGACAACCCAGAGCGGTTCGACCCCGGCCCTTGCGTGCTGGGCCAGGAG
  CGCATTACCTCTGGCCGCCACTACTGGGAGGTGGAAGTCGGGGACCAGACCAGCTGGGCACTCGGCGTGT
  GTAAAGAAACTGCCAACAGGAAGGAGAAGGGGGAGCTGTCGGCTGGCAATGGGTTCTGGATCCTGGTGTT
  CCTGGGGAGTTTCTATAATTCCAATGAGCCGGCCTTCTCCCCACTGCGGGACCCTCCCAAGCGTGTGGGG
  ATTTTTCTGGACTATGAAGCTGGCCATCTCTCATTCTACAGTGCCACGGATGGGTCGCTGCTGTTTATCT
  TCCCCGAGACCCTGTTCTCAGGGACACTCCGGCCCCTCTTCTCACCTCTGTCAAGCAGCCCGACCCCTAT
  GACTATCTGCAGGCTGATAGGTGTATCTGGGGACACCCTTGGCCCGCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230145 representing NM_001290988
  Red=Cloning site Green=Tags(s)
  
  MAAPDLSTNLQEEATCAICLDYFTDPVMTDCGHNFCRECIRRCWGQPEGPYACPECRELSAQRNLRPNRP
  LAKMAEMARRLHPPSPVPQGVCAAHREPLTTFCGDDLSLLCPICERSEHWTHRVRPLQEAADDLKGRLEK
  SLEHLRKQMEDAMLFQAQAEETCALWQKMVESQRQNVLGEFERLRRLLAEEEQQLLQKLEEEELEVLPRL
  REGAARLGQQSTQLAALISELESRCQLPALGLLQLCIECCALEREASIAKDIKDALCRVQDVKLQPPAVV
  PMELRTVCRVPGLVETLRRFRGDITLDPDTANPELVLSEDRRSVQRGEQRQALPDNPERFDPGPCVLGQE
  RITSGRHYWEVEVGDQTSWALGVCKETANRKEKGELSAGNGFWILVFLGSFYNSNEPAFSPLRDPPKRVG
  IFLDYEAGHLSFYSATDGSLLFIFPETLFSGTLRPLFSPLSSSPTPMTICRLIGVSGDTLGPQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290988
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290988.1, NP_001277917.1
• RefSeq Size: 2327 bp
• RefSeq ORF: 1452 bp
• Locus ID: 94091
• UniProt ID: Q99PQ2
• Cytogenetics: 11 B1.3
• MW: 54.8 kDa
• Gene Summary: E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.[UniProtKB/Swiss-Prot Function]