Rgs6 (NM_001282061) Mouse Tagged ORF Clone

CAT#: MR230076

Rgs6 (myc-DDK-tagged) - Mouse regulator of G-protein signaling 6 (Rgs6), transcript variant 2

Product Images

Circular map for MR230076

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rgs6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230076 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGGGGTCCGGGGACCAGCGAGCAGTGGGGATCGCTGATCCAGAAGAGAGTTCTCCCAACATGA
  TTGTCTACTGCAAAATTGAGGACATCATTACAAAGATGCAAGATGACAAGACAGGGGGTGTGCCCATCAG
  AACAGTTAAGAGCTTTCTCTCCAAAATCCCCAGTGTCGTCACAGGTACTGACATTGTACAGTGGCTTATG
  AAGAACCTTTCCATTGAGGACCCAGTTGAAGCAATACACCTGGGAAGCCTTATTGCCGCCCAGGGCTACA
  TCTTCCCAATCTCAGACCATGTTCTCACCATGAAGGACGATGGCACCTTTTACCGTTTCCAGGCTCCTTA
  CTTCTGGCCTTCAAACTGCTGGGAACCTGAAAACACGGACTATGCCATCTATCTCTGTAAGAGGACGATG
  CAGAACAAAGCAAGGCTGGAACTGGCCGACTACGAAGCAGAAAACTTAGCAAGACTCCAGAGGGCCTTTG
  CAAGGAAGTGGGAATTCATCTTTATGCAAGCAGAAGCACAAGTGAAGATTGACCGGAAAAAGGATAAGAC
  AGAAAGAAAAATTCTGGATAGCCAAGAACGGGCCTTCTGGGATGTCCACAGGCCAGTGCCAGGCTGTGTG
  AACACAACAGAAATGGATATCAGAAAATGTCGGCGTTTGAAGAATCCACAAAAGGTTAAAAAGTCAGTAT
  ATGGTGTGACAGACGAGACCCAGTCACAGAGTCCAGTGCACATACCAAGCCAGCCAATCAGGAAAACTAC
  AAAAGATGACATCCGAAAACAGATAACGTTTTTGAATGCACAGATTGACAGACATTGTTTGAAAATGTCC
  AAAGTGGCTGAAAGTTTAATCGCTTACACGGAGCAGTATGTGGAGTACGACCCATTCATAACACCAGCAG
  AGCCATCTAATCCTTGGATCAGCGATGACATCACCTTATGGGACATAGAGATGAGCAAAGAGCCCAGCCA
  GCAGCGAGTGAAGCGTTGGGGCTTCTCTTTTGATGAGATACTGAAGGACCAGGTGGGGCGGGACCAGTTC
  CTCAGATTCCTGGAGTCAGAATTCAGCTCAGAAAATCTCAGGTTCTGGCTGTCTGTCCAAGATCTCAAGA
  AGCAACCTCTACAGGACGTGGCCAAGAGGGTGGAGGAAATCTGGCAAGAGTTTCTAGCTCCCGGAGCCCC
  AAGTGCAATCAACTTGGATTCTCACAGCTATGAGATAACCAGTCAGAATGTCAAAGATGGAGGGAGATAC
  ACATTTGAAGATGCCCAGGAGCACATCTACAAGCTGATGAAGAGTGACAGCTATGCCCGCTTCCTACGGT
  CCAACGCTTACCAGGATCTGTTGCTGGCCAAGAAGAAGGGAAAGTCGCTGGCGGGCAAGCGCCTTACGGG
  CCTGATGCAGTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230076 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQGSGDQRAVGIADPEESSPNMIVYCKIEDIITKMQDDKTGGVPIRTVKSFLSKIPSVVTGTDIVQWLM
  KNLSIEDPVEAIHLGSLIAAQGYIFPISDHVLTMKDDGTFYRFQAPYFWPSNCWEPENTDYAIYLCKRTM
  QNKARLELADYEAENLARLQRAFARKWEFIFMQAEAQVKIDRKKDKTERKILDSQERAFWDVHRPVPGCV
  NTTEMDIRKCRRLKNPQKVKKSVYGVTDETQSQSPVHIPSQPIRKTTKDDIRKQITFLNAQIDRHCLKMS
  KVAESLIAYTEQYVEYDPFITPAEPSNPWISDDITLWDIEMSKEPSQQRVKRWGFSFDEILKDQVGRDQF
  LRFLESEFSSENLRFWLSVQDLKKQPLQDVAKRVEEIWQEFLAPGAPSAINLDSHSYEITSQNVKDGGRY
  TFEDAQEHIYKLMKSDSYARFLRSNAYQDLLLAKKKGKSLAGKRLTGLMQSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282061
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282061.2, NP_001268990.1
• RefSeq Size: 2345 bp
• RefSeq ORF: 1419 bp
• Locus ID: 50779
• UniProt ID: Q9Z2H2
• Cytogenetics: 12 38.14 cM
• MW: 54.5 kDa
• Gene Summary: This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP (Dishevelled, Egl-10, and Pleckstrin) and GGL (G-protein gamma like)domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Mice lacking this gene exhibit decreased heart rate. Alternative splicing results in multiple transcript variants, however, the full-length nature of some of these variants is not known. [provided by RefSeq, Sep 2015]