Rgs6 (NM_001282061) Mouse Tagged ORF Clone
CAT#: MR230076
- TrueORF®
Rgs6 (myc-DDK-tagged) - Mouse regulator of G-protein signaling 6 (Rgs6), transcript variant 2
AAV Particle: DDK
"NM_001282061" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rgs6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230076 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGGGGTCCGGGGACCAGCGAGCAGTGGGGATCGCTGATCCAGAAGAGAGTTCTCCCAACATGA TTGTCTACTGCAAAATTGAGGACATCATTACAAAGATGCAAGATGACAAGACAGGGGGTGTGCCCATCAG AACAGTTAAGAGCTTTCTCTCCAAAATCCCCAGTGTCGTCACAGGTACTGACATTGTACAGTGGCTTATG AAGAACCTTTCCATTGAGGACCCAGTTGAAGCAATACACCTGGGAAGCCTTATTGCCGCCCAGGGCTACA TCTTCCCAATCTCAGACCATGTTCTCACCATGAAGGACGATGGCACCTTTTACCGTTTCCAGGCTCCTTA CTTCTGGCCTTCAAACTGCTGGGAACCTGAAAACACGGACTATGCCATCTATCTCTGTAAGAGGACGATG CAGAACAAAGCAAGGCTGGAACTGGCCGACTACGAAGCAGAAAACTTAGCAAGACTCCAGAGGGCCTTTG CAAGGAAGTGGGAATTCATCTTTATGCAAGCAGAAGCACAAGTGAAGATTGACCGGAAAAAGGATAAGAC AGAAAGAAAAATTCTGGATAGCCAAGAACGGGCCTTCTGGGATGTCCACAGGCCAGTGCCAGGCTGTGTG AACACAACAGAAATGGATATCAGAAAATGTCGGCGTTTGAAGAATCCACAAAAGGTTAAAAAGTCAGTAT ATGGTGTGACAGACGAGACCCAGTCACAGAGTCCAGTGCACATACCAAGCCAGCCAATCAGGAAAACTAC AAAAGATGACATCCGAAAACAGATAACGTTTTTGAATGCACAGATTGACAGACATTGTTTGAAAATGTCC AAAGTGGCTGAAAGTTTAATCGCTTACACGGAGCAGTATGTGGAGTACGACCCATTCATAACACCAGCAG AGCCATCTAATCCTTGGATCAGCGATGACATCACCTTATGGGACATAGAGATGAGCAAAGAGCCCAGCCA GCAGCGAGTGAAGCGTTGGGGCTTCTCTTTTGATGAGATACTGAAGGACCAGGTGGGGCGGGACCAGTTC CTCAGATTCCTGGAGTCAGAATTCAGCTCAGAAAATCTCAGGTTCTGGCTGTCTGTCCAAGATCTCAAGA AGCAACCTCTACAGGACGTGGCCAAGAGGGTGGAGGAAATCTGGCAAGAGTTTCTAGCTCCCGGAGCCCC AAGTGCAATCAACTTGGATTCTCACAGCTATGAGATAACCAGTCAGAATGTCAAAGATGGAGGGAGATAC ACATTTGAAGATGCCCAGGAGCACATCTACAAGCTGATGAAGAGTGACAGCTATGCCCGCTTCCTACGGT CCAACGCTTACCAGGATCTGTTGCTGGCCAAGAAGAAGGGAAAGTCGCTGGCGGGCAAGCGCCTTACGGG CCTGATGCAGTCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230076 protein sequence
Red=Cloning site Green=Tags(s) MAQGSGDQRAVGIADPEESSPNMIVYCKIEDIITKMQDDKTGGVPIRTVKSFLSKIPSVVTGTDIVQWLM KNLSIEDPVEAIHLGSLIAAQGYIFPISDHVLTMKDDGTFYRFQAPYFWPSNCWEPENTDYAIYLCKRTM QNKARLELADYEAENLARLQRAFARKWEFIFMQAEAQVKIDRKKDKTERKILDSQERAFWDVHRPVPGCV NTTEMDIRKCRRLKNPQKVKKSVYGVTDETQSQSPVHIPSQPIRKTTKDDIRKQITFLNAQIDRHCLKMS KVAESLIAYTEQYVEYDPFITPAEPSNPWISDDITLWDIEMSKEPSQQRVKRWGFSFDEILKDQVGRDQF LRFLESEFSSENLRFWLSVQDLKKQPLQDVAKRVEEIWQEFLAPGAPSAINLDSHSYEITSQNVKDGGRY TFEDAQEHIYKLMKSDSYARFLRSNAYQDLLLAKKKGKSLAGKRLTGLMQSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001282061 |
ORF Size | 1419 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282061.2, NP_001268990.1 |
RefSeq Size | 2345 bp |
RefSeq ORF | 1419 bp |
Locus ID | 50779 |
UniProt ID | Q9Z2H2 |
Cytogenetics | 12 38.14 cM |
MW | 54.5 kDa |
Gene Summary | This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP (Dishevelled, Egl-10, and Pleckstrin) and GGL (G-protein gamma like)domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Mice lacking this gene exhibit decreased heart rate. Alternative splicing results in multiple transcript variants, however, the full-length nature of some of these variants is not known. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227865 | Rgs6 (untagged) - Mouse regulator of G-protein signaling 6 (Rgs6), transcript variant 2 |
USD 503.00 |
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