Mid1 (NM_001290512) Mouse Tagged ORF Clone

CAT#: MR230028

Mid1 (myc-DDK-tagged) - Mouse midline 1 (Mid1), transcript variant 6

Product Images

Circular map for MR230028

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mid1
• Synonyms: 61B3-R; DXHXS1141; Fxy; Trim18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230028 representing NM_001290512
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTTGCACAAGGATGCATTTTCAAAGCTTGACACTGAGCAAAACTTGGAGAGTAATCTCACCAATC
  TTATTAAGAGAAACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAAGT
  CAATGCATCCCGTCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAATCATTCAGCAACGA
  AGACAAATTATTGGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTTAGCTCAGCAGATTG
  CAAACTGTAAACAGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCACTCGCTGAAGGAAAA
  TGACCACGCCCGTTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCC
  CAGGTCCTAATTCCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGATTTTTCCCGGGAGA
  AGAAACTGCTAGAATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGAGAAGAGCTCTGCAC
  CGCTTCCTACGACACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGGTCTCCTACGAGCTC
  CAGTACACCATATTCACCGGACAAGCCAATGTTGTCAGTCTGTGTAACTCGGCGGACAGCTGGATGATCG
  TGCCCAACATCAAGCAGAACCACTACACCGTGCACGGCCTGCAAAGTGGCACCAAGTATATCTTCACGGT
  GAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGAAAGCTGAAGACCAACAGTCAGCCGTTT
  AGACTGGATCCCAAATCGGCTCATCGCAAGCTGAAGGTGTCCCACGACAACCTGACTGTCGAGCGCGACG
  AGTCGTCCTCTAAGAAGAGTCACGCGCCGGAGCGCTTCGCTGGTCAGGGGAGCTACGGAGTGGCTGGCAA
  CGTGTTCATCGACAGCGGCCGTCACTACTGGGAAGTGGTCACCAGCGGAAGCACATGGTACGCCATCGGC
  CTGGCGTACAGATCGGCGCCGAAACACGAGTGGATCGGGAAGAACGCGGCGTCCTGGGCCCTCTGCCGCT
  GCCACAACCACTGGGCGGTGCGACACGACGGCAAGGAGACCCCCATCGCGCCGGCCCCTCACCTCAGGCG
  CGTCGGCGTCCTGCTGGACTACGACAACGGCTCCATCGCCTTCTACGACGCTCTGAGCTCCGTCCACCTC
  CACACCTTCCACGCGGCGCTCGCGCAGCCCGTGTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACCA
  TCGTCACGGGTCTGCCCATCCCGGACCATCTGGACTGTACGGAGCAGCGACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230028 representing NM_001290512
  Red=Cloning site Green=Tags(s)
  
  MTLHKDAFSKLDTEQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQR
  RQIIGTKIKEGKVIRLRKLAQQIANCKQCLERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASS
  QVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPAIREELCTASYDTITVHWTSEDEFSVVSYEL
  QYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFTVKAINQAGSRSSEPGKLKTNSQPF
  RLDPKSAHRKLKVSHDNLTVERDESSSKKSHAPERFAGQGSYGVAGNVFIDSGRHYWEVVTSGSTWYAIG
  LAYRSAPKHEWIGKNAASWALCRCHNHWAVRHDGKETPIAPAPHLRRVGVLLDYDNGSIAFYDALSSVHL
  HTFHAALAQPVCPTFTVWNKCLTIVTGLPIPDHLDCTEQRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290512
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290512.1, NP_001277441.1
• RefSeq Size: 3158 bp
• RefSeq ORF: 1386 bp
• Locus ID: 17318
• Cytogenetics: X 79.19 cM
• MW: 52.3 kDa
• Gene Summary: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.[UniProtKB/Swiss-Prot Function]