Etfbkmt (NM_001252096) Mouse Tagged ORF Clone
CAT#: MR228812
- TrueORF®
Mettl20 (myc-DDK-tagged) - Mouse methyltransferase like 20 (Mettl20), transcript variant 3
AAV Particle: DDK
"NM_001252096" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Etfbkmt |
Synonyms | 4833442J19Rik; AI256744; ETFB-KMT; Mettl20 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228812 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCAGCCTGTGTTGGAAAGCTCCCAGGAGCCCATGGAGTTTCCTGCAGGCTGTGAACAATGGGT CTCCCCTGTTTCTCTGGAGGACAGTGGGGAGCTGTTTGGATCCTAAGATGAAAGCCTACCTGGAAGAGAA CACTGAAGTCACCAGCAGTGGTAGCCTTACCCCGGAAATCCAGTTACGGCTTTTAACCCCCAGGTGCAAG TTTTGGTGGGAAAGGGCTGACCTGTGGCCCTACAGTGATCCCTACTGGGCTATTTACTGGCCAGGAGGCC AGGCTCTGTCTAGGTATCTTTTGGATAACCCTGCTGTTGTTAGAGGAAAGTCTGTCTTAGATCTTGGGAG TGGATGTGGAGCTACAGCCATTGCTGCCAAGATGAGTGGAGCATCAAAGATCTTGGCCAACGACATAGAC CCCATTGCAGGAATGGCGATTACACTAAATTGCAAATTAAATGGACTGAATCCTTTTCCTGTTTTAACTA AAAACATTTTGAATACCCAACAAGGTAAGTTTGATCTCATTGTTCTTGGAGATATGTTTTATGATGAAGA CCTTGCAGACAGTCTTCATCTGTGGCTGCAGAACTACTTTTGGACCCATGGAACCCGAGTACTGATTGGT GACCCTGGGAGGCCCCAGTTCAGTGGACATAGCATTCGGCATCAACTGTACCAACTAGTAGAATACACAC TTCCAGAGCCAACTCAGCAAGAGAACAATGGACTGACCACAAGTGCTGTATGGGATTTTCATCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228812 protein sequence
Red=Cloning site Green=Tags(s) MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCK FWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDID PIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIG DPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001252096 |
ORF Size | 768 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001252096.1, NP_001239025.1 |
RefSeq Size | 1597 bp |
RefSeq ORF | 768 bp |
Locus ID | 320204 |
UniProt ID | Q80ZM3 |
Cytogenetics | 6 G3 |
MW | 28.6 kDa |
Gene Summary | Protein-lysine methyltransferase that selectively trimethylates the flavoprotein ETFB in mitochondria. Thereby, may negatively regulate the function of ETFB in electron transfer from Acyl-CoA dehydrogenases.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226601 | Mettl20 (untagged) - Mouse methyltransferase like 20 (Mettl20), transcript variant 3 |
USD 330.00 |
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