Cbln2 (NM_001302356) Mouse Tagged ORF Clone

CAT#: MR228603

Cbln2 (myc-DDK-tagged) - Mouse cerebellin 2 precursor protein (Cbln2), transcript variant 2

Product Images

Circular map for MR228603

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbln2
• Synonyms: 6330593N19Rik; A730004O05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228603 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGCGCCTGGCCAGGGCCCCAGAGGGCCGCTGCTGAGCATGCCCGGGCGCCGGGGGGCGCTGCGTG
  AGCCAGCCGACTTTGGCTCCAGCCTGGGGGCGGTGCTGGCCCTGCTGTTGCTGCTGCTGCCCGCCTGCTG
  CCCCGTAAGGGCTCAGAACGACACGGAGCCCATCGTGCTAGAGGGCAAGTGCCTGGTAGTGTGCGATTCC
  AGCCCATCGGGGGATGGCGCCGTCACTTCTTCCCTGGGCATTTCTGTGCGCTCAGGCAGTGCCAAGGTGG
  CCTTCTCCGCTACTCGGAGCACCAACCACGAGCCGTCAGAGATGAGCAACCGTACCATGACCATCTACTT
  CGACCAGGTCTTAGTAAACATTGGCAACCACTTTGACCTTGCCTCCAGTATATTTGTAGCACCAAGAAAG
  GGAATTTATAGCTTCAGCTTCCACGTGGTCAAAGTGTACAACAGACAAACTATCCAGGTCAGCTTAATGC
  AGAATGGCTACCCGGTGATCTCTGCATTTGCCGGAGACCAGGATGTTACCAGGGAAGCAGCCAGCAATGG
  TGTTCTGCTGCTCATGGAAAGAGAAGACAAAGTTCATCTCAAACTAGAGAGAGGCAACCTCATGGGAGGC
  TGGAAATACTCCACATTCTCGGGCTTCTTGGTTTTTCCTCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228603 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPAPGQGPRGPLLSMPGRRGALREPADFGSSLGAVLALLLLLLPACCPVRAQNDTEPIVLEGKCLVVCDS
  SPSGDGAVTSSLGISVRSGSAKVAFSATRSTNHEPSEMSNRTMTIYFDQVLVNIGNHFDLASSIFVAPRK
  GIYSFSFHVVKVYNRQTIQVSLMQNGYPVISAFAGDQDVTREAASNGVLLLMEREDKVHLKLERGNLMGG
  WKYSTFSGFLVFPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302356
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302356.2
• RefSeq Size: 2524 bp
• RefSeq ORF: 675 bp
• Locus ID: 12405
• UniProt ID: Q8BGU2
• Cytogenetics: 18 58.63 cM
• MW: 24.1 kDa
• Gene Summary: The protein encoded by this gene belongs to a family of secreted neuronal glycoproteins. The transcript is broadly expressed in the embryonic and adult brain with higher levels in some regions including the olfactory bulb, thalamus, and cerebral cortex. The protein can bind to presynaptic neurexins and induce synaptogenesis in cultured neurons. Null mutant mice are viable, fertile and do not display obvious neuroanatomical defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]