Acy3 (NM_001302482) Mouse Tagged ORF Clone

CAT#: MR228382

Acy3 (myc-DDK-tagged) - Mouse aspartoacylase (aminoacylase) 3 (Acy3), transcript variant 4

Product Images

Circular map for MR228382

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acy3
• Synonyms: 0610006H10Rik; A; AA3; AAIII; Acy-3; AW107362; HCBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228382 representing NM_001302482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCCCTACCTGGGTCCCGGGAGCCCCTGCTCCGTGTGGCTGTGACTGGAGGCACCCACGGGAATG
  AGATGTGTGGTGTCTACCTGGCCCGGTACTGGCTACAGAACCCAGGGGAGCTGCAGAGACCCAGCTTCTC
  AGCCATGCCGGTTCTGGCCAACCCAGCAGCCACAGCTGCCTGTTGCCGTTACCTGGACCGTGATCTCAAC
  CGCTCCTGCACCCTCACCTTCCTTGGCGGCAGAACCCGGGGATGCCCTGCCGCCTCTTTCTGTATGAGCC
  AGCTGGGACGGAGACCTTCAGCGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCACAG
  CCTCAGGGCGTGCTGCGGGCCGACCTGTTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCA
  TCGAGCTCTTCAACCAAGGCATGGACTTACCCGCCTTTGAGATGGATATCTACAGGAACTTGGGCAGTGT
  GGACTTCCCACGCACTGCAGATGGTGACCTGGCTGGCACTGTGCACCCTCAACTGCAGGACCATGACTTT
  GAGCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228382 representing NM_001302482
  Red=Cloning site Green=Tags(s)
  
  MSSLPGSREPLLRVAVTGGTHGNEMCGVYLARYWLQNPGELQRPSFSAMPVLANPAATAACCRYLDRDLN
  RSCTLTFLGGRTRGCPAASFCMSQLGRRPSAWNLYPRMESVWRWAHSLRACCGPTCSPGCELWWHPFWTS
  SSSSTKAWTYPPLRWISTGTWAVWTSHALQMVTWLALCTLNCRTMTLSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302482
• ORF Size: 567 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302482.1, NP_001289411.1
• RefSeq Size: 994 bp
• RefSeq ORF: 570 bp
• Locus ID: 71670
• UniProt ID: Q91XE4
• Cytogenetics: 19 A
• MW: 21.6 kDa
• Gene Summary: This gene encodes a member of the aminoacylase family of enzymes. This enzyme specifically deacetylates N-acetyl aromatic amino acids and mercapturic acids. Action of this enzyme on metabolites of the environmental contaminant trichloroethylene leads to the generation of toxic products that may lead to kidney failure. This protein has been found to bind to the hepatitis C virus core protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]