Banf1 (NM_001286608) Mouse Tagged ORF Clone
CAT#: MR227783
- TrueORF®
Banf1 (myc-DDK-tagged) - Mouse barrier to autointegration factor 1 (Banf1), transcript variant 3
AAV Particle: DDK
"NM_001286608" in other vectors (1)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Banf1 |
Synonyms | Baf; Bcrp1; L2bp1/Baf |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227783 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACCTCCCAAAAGCACCGAGACTTCGTGGCAGAGCCCATGGGGGAAAAGCCAGTGGGGAGCCTGG CCGGGATTGGTGACGTCCTGAGCAAGAGGCTGGAGGAAAGGGGCTTTGACAAGGCTTATGTGGTCCTTGG CCAGTTTCTGGTGCTAAAGAAAGATGAAGACCTCTTCCGAGAATGGCTGAAGGATACATGTGGTGCCAAT GCCAAGCAGTCCCGGGACTGCTTTGGGTGCCTTCGAGAATGGTGTGATGCCTTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227783 protein sequence
Red=Cloning site Green=Tags(s) MTTSQKHRDFVAEPMGEKPVGSLAGIGDVLSKRLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGAN AKQSRDCFGCLREWCDAFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001286608 |
ORF Size | 270 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286608.1, NP_001273537.1 |
RefSeq Size | 1108 bp |
RefSeq ORF | 270 bp |
Locus ID | 23825 |
UniProt ID | O54962 |
Cytogenetics | 19 A |
MW | 10.1 kDa |
Gene Summary | Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC225572 | Banf1 (untagged) - Mouse barrier to autointegration factor 1 (Banf1), transcript variant 3 |
USD 165.00 |
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