Tmem102 (NM_001033433) Mouse Tagged ORF Clone

CAT#: MR227686

Tmem102 (Myc-DDK-tagged) - Mouse transmembrane protein 102 (Tmem102)

Product Images

Circular map for MR227686

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem102
• Synonyms: 4632403M07Rik; Cbap; Tmem102-ps
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227686 representing NM_001033433
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCACCGTCTGGGGGGGTGCCCCCTGGTGGGGACCACCGCCCCCAGCCCCAGCCCGGCCTCTCA
  CAGACATAGATTTCTGCTCGGGCGCCCAGCTGCAGGAGCTCACTCAGTTAATTCAGGAGCTCCGTGTTCA
  GGAGAGTTGGAGTGAAGGGCCCAAGCCTGGAGCTGATCTTCTCCGAGCCAAGGATTTTGTCTTCGCTCTA
  CTCGGTCTGGTTCACCGCCAAGACCCTCGTTTTCCACCCCAGGCAGAACTCTTGCTGCTTCGTGGTGGGA
  TTCGCGAAGGTTCCCTAGATTTAGGGCACGCCCCCTTAGGTCCCTACTCCCGGGGACCTCATTATGATGC
  CGGCTTTACACTCCTTGTGCCTGTGTTTTCTCTGGATGGCACTGGGCCCGAGCTGCTACTGGATCTGGAA
  TCCTGTTCTGCATGGCTCCGCCTTCCAGAACTGATGCGTGGAATTTTGGTCCGGGAGGCCTGGCAGGATT
  GCTTAGGACCTCCTGTCCCAGAAGAAAGTGATATGACTCACCAAACCCATAGCAAAGAGAGTCCCACCGA
  CCGGGAAAACTCTGTAGATCCGTCACATGATTATGTCCCTGAACCTGAGCCACACATGTCTTTGCAAAAA
  TCATCTAGTGACCTTTCAGAGTCCCAATCGTCTTATAAGGACATTACCAATCCTGAAACTCCTGAGCCAT
  TGGAAACACTGAGCAGCGACGCCTTGGACGCCGACGAAAGCCAAGTCCCAAAGCCGTCGGAGGCTCCAAA
  GGCATGGCCCACATTATGCCCGACCCAAGTAACTTCCTGGTTCTTCGTTAAGTTGGCTGAGGTTGCTGAG
  TCCCTGATCCCGGTCCCAGGCGCCCCACGGCTAGTTCATGCAGCTCGTCACGCAGGTGTCACCACGGTCC
  TCCTGGCAACTCCAGAGCCCCCACGCCACCTCCTGCTCTTCGACTTGATCCCGGTGGTGACCGTGACAGG
  CTGGCCTGACACGGCTCGGAGCCACTCGTGGGCGGGTCCACTGGTCTCTGAATCGGCCTCTTTCTACCTA
  GTGCCGGGCAGCCTCCCAGAGCAGCCGAGCACCTCTGGATGGCAGCTGTGCTTCGCTCGCCAGGAGCTGG
  CGCTCAAAGAGCGCATCCCCACGCCGCTGCTTCAGGCACACGCGGCGGCCCAGGCGCTGCTGCGCCCGCT
  GGTGGCGGGGACCCGAGCCGCTGCGCCCTACCTCCTGCGGACCTTGCTCTACTGGGCGTGCGAGCGGCTC
  CCGGCGCTCTACTTGGCGCGGCCGGAAAATGCCGGCGCTTGTTGCCTTGGGCTACTGGATGAGCTGAGCC
  GAGTGCTAGAGGCCGGGGCGCTGCCTCATTATTTTTTGAGCGGCCGAAAGCTCCGTGTGGGAGACGGCTC
  CGCTGCTCTGCGCGGGGCATTGGCCCAGCTCCGCGGGGACCCTGCACAGGCCTTGCGTGAGGCTGTAGAG
  GAAGCCAAGGTTGCCCGCAAAGGGGGCGGTTTAGCGGGCGTAGGGGGCGGGACCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227686 representing NM_001033433
  Red=Cloning site Green=Tags(s)
  
  MASTVWGGAPWWGPPPPAPARPLTDIDFCSGAQLQELTQLIQELRVQESWSEGPKPGADLLRAKDFVFAL
  LGLVHRQDPRFPPQAELLLLRGGIREGSLDLGHAPLGPYSRGPHYDAGFTLLVPVFSLDGTGPELLLDLE
  SCSAWLRLPELMRGILVREAWQDCLGPPVPEESDMTHQTHSKESPTDRENSVDPSHDYVPEPEPHMSLQK
  SSSDLSESQSSYKDITNPETPEPLETLSSDALDADESQVPKPSEAPKAWPTLCPTQVTSWFFVKLAEVAE
  SLIPVPGAPRLVHAARHAGVTTVLLATPEPPRHLLLFDLIPVVTVTGWPDTARSHSWAGPLVSESASFYL
  VPGSLPEQPSTSGWQLCFARQELALKERIPTPLLQAHAAAQALLRPLVAGTRAAAPYLLRTLLYWACERL
  PALYLARPENAGACCLGLLDELSRVLEAGALPHYFLSGRKLRVGDGSAALRGALAQLRGDPAQALREAVE
  EAKVARKGGGLAGVGGGTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033433
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033433.4, NP_001028605.2
• RefSeq Size: 1941 bp
• RefSeq ORF: 1530 bp
• Locus ID: 380705
• UniProt ID: Q3UPR7
• Cytogenetics: 11 B3
• MW: 54.9 kDa
• Gene Summary: Selectively involved in CSF2 deprivation-induced apoptosis via a mitochondria-dependent pathway.[UniProtKB/Swiss-Prot Function]