Itgb8 (NM_177290) Mouse Tagged ORF Clone

CAT#: MR227668

Itgb8 (Myc-DDK-tagged) - Mouse integrin beta 8 (Itgb8)

Product Images

Circular map for MR227668

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgb8
• Synonyms: 4832412O06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227668 representing NM_177290
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGGCTCGGCCCTGGCTTTTCTGACTGCTGCACTGCTCTCTCTGCACAACTGCCAGCGAGGTCCAG
  CCTTGGTTCTTGGGGCAGCCTGGGTATTTTCACTTGTTCTCGGACTGGGCCAAAGTGAACACAATAGATG
  TGGCTCTGCAAATGTGGTCTCCTGTGCCAGGTGCCTCCAGCTGGGTCCGGAATGCGGATGGTGTGTTCAA
  GAGGATTTTGTTTCAGGTGGATCAGGAAGTGAACGTTGTGATACTGTTTCCAGTTTGATAAGCAAAGGCT
  GTCCAGTTGATTCAATAGAATACCTGTCTGTGCATGTTGTAACGTCAAGTGAGAATGAAATCAATACCCA
  GGTGACACCGGGAGAAGTCTCAGTCCAACTGCATCCAGGAGCTGAAGCTAATTTTATGTTAAAAGTCCGT
  CCTCTGAAGAAATACCCCGTGGATCTTTATTATCTGGTTGATGTGTCAGCATCAATGCACAATAATATAG
  AAAAATTGAATTCCGTTGGAAATGATTTATCTAAAAAAATGGCCCTTTATTCCCGTGACTTCCGTCTTGG
  TTTTGGCTCATATGTGGATAAAACTGTCTCGCCATACATTAGTATCCACCCGGAAAGGATCCACAATCAG
  TGCAGTGACTACAATTTAGACTGTATGCCTCCCCATGGATACATTCATGTGCTGTCTCTGACAGAAAACA
  TCACTGAGTTTGAAAAAGCAGTCCACAGACAAAAGATCTCTGGAAACATAGACACCCCTGAAGGAGGTTT
  TGATGCCATGCTTCAGGCTGCCGTCTGTGAGAGTCATATTGGATGGCGAAAAGAAGCTAAAAGATTGCTG
  CTGGTGATGACAGACCAGACATCACATCTTGCTCTTGACAGCAAATTGGCAGGCATAGTGGTGCCGAATG
  ACGGAAACTGCCATCTCAAAAACAATGTCTACGTCAAATCGACAACCATGGAACATCCGTCATTAGGCCA
  ACTTTCTGAAAAGTTAATAGACAACAACATAAATGTCATTTTTGCAGTTCAAGGAAAGCAGTTTCATTGG
  TACAAGGACCTTCTGCCCCTTTTGCCTGGTGCCATTGCTGGTGAAATAGAATCCAAAGCTGCAAATCTCA
  ACAATTTAGTAGTAGAAGCCTACAAGAAGATTATCTCAGAAGTAAAAGTGCAGCTGGAAAACCAGGTACA
  CGGTGTCCATTTTAACATCACTGCCATCTGTCCAGATGGGGCCAGAAAGCCAGGCATAAGTGGGTGTGGA
  AACGTGACAAGCAATGACGAAGTTCTTTTCAATGTAACTGTTGTGATGAAAACATGTGATATCATGGGAG
  GAAAAAACTATGCAATAATCAAGCCTATTGGTTTCAATGAAACCACTAAAGTCCACATACACAGAAGCTG
  CAGTTGCCAGTGTGAGAACCACAGAGGACTCAAAGGACAGTGTGCGGAAGCTGCCCCAGACCCCAAGTGT
  CCACAGTGTGATGACAGCAGATGTCATTTTGATGAAGACCAGTTTCCTTCTGAAACTTGCAAGCCACAGG
  AGGATCAACCTGTCTGCAGTGGCCGAGGTGTTTGCATCTGTGGGAAATGTTTATGTCACAAAACCAAGCT
  AGGAAGAGTGTATGGCCAGTACTGTGAGAAGGATGACTTCTCCTGTCCCTATCTCCATGGGGATGTGTGT
  GCTGGGCATGGGGAGTGTGAAGGTGGCAGATGCCAGTGCTTTAGTGGCTGGGAAGGAGATCGGTGTCAGT
  GCCCATCAGCCTCAGCACAGCACTGTGTCAACTCCAAGGGCCAAGTTTGCAGCGGGAGAGGCACCTGCGT
  GTGTGGCAGGTGTGAGTGTACTGATCCCAGAAGCATTGGCCGTCTCTGTGAGCACTGCCCAACCTGCCAT
  CTCTCCTGCAGTGAAAACTGGAATTGTCTGCAGTGCCTTCACCCTCACAATCTGTCTCAGGCTGCCCTTG
  ATCAGTGTAAATCCTCCTGTGCTGTCATGGAACAGCATCGCATGGACCAAACATCAGAATGTTTATCTGG
  CCCAAGCTATCTGCGAATATTTTTCATCATATTCATAGTCACATTCTTGATCGGGTTGCTTAAAGTTCTT
  ATCATTAGACAAGTGATACTACAATGGAATAATAATAAAATAAAGTCCTCATCAGATTATAGAATGTCTG
  CTTCTAAAAAGGATAAACTGATTCTGCAAAGTGTTTGCACGAGAGCTGTAACCTACCGACGAGAAAAGCC
  TGAGGAAATAAAAATGGATATCAGCAAACTAAATGCTCAGGAGGCCTTCAGGTGCAACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227668 representing NM_177290
  Red=Cloning site Green=Tags(s)
  
  MCGSALAFLTAALLSLHNCQRGPALVLGAAWVFSLVLGLGQSEHNRCGSANVVSCARCLQLGPECGWCVQ
  EDFVSGGSGSERCDTVSSLISKGCPVDSIEYLSVHVVTSSENEINTQVTPGEVSVQLHPGAEANFMLKVR
  PLKKYPVDLYYLVDVSASMHNNIEKLNSVGNDLSKKMALYSRDFRLGFGSYVDKTVSPYISIHPERIHNQ
  CSDYNLDCMPPHGYIHVLSLTENITEFEKAVHRQKISGNIDTPEGGFDAMLQAAVCESHIGWRKEAKRLL
  LVMTDQTSHLALDSKLAGIVVPNDGNCHLKNNVYVKSTTMEHPSLGQLSEKLIDNNINVIFAVQGKQFHW
  YKDLLPLLPGAIAGEIESKAANLNNLVVEAYKKIISEVKVQLENQVHGVHFNITAICPDGARKPGISGCG
  NVTSNDEVLFNVTVVMKTCDIMGGKNYAIIKPIGFNETTKVHIHRSCSCQCENHRGLKGQCAEAAPDPKC
  PQCDDSRCHFDEDQFPSETCKPQEDQPVCSGRGVCICGKCLCHKTKLGRVYGQYCEKDDFSCPYLHGDVC
  AGHGECEGGRCQCFSGWEGDRCQCPSASAQHCVNSKGQVCSGRGTCVCGRCECTDPRSIGRLCEHCPTCH
  LSCSENWNCLQCLHPHNLSQAALDQCKSSCAVMEQHRMDQTSECLSGPSYLRIFFIIFIVTFLIGLLKVL
  IIRQVILQWNNNKIKSSSDYRMSASKKDKLILQSVCTRAVTYRREKPEEIKMDISKLNAQEAFRCNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177290
• ORF Size: 2301 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177290.3, NP_796264.2
• RefSeq Size: 3096 bp
• RefSeq ORF: 2304 bp
• Locus ID: 320910
• UniProt ID: Q0VBD0
• Cytogenetics: 12 F2
• MW: 85 kDa
• Gene Summary: Integrin alpha-V:beta-8 (ITGAV:ITGB8) is a receptor for fibronectin (By similarity). It recognizes the sequence R-G-D in its ligands (By similarity). Integrin alpha-V:beta-6 (ITGAV:ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation on the surface of activated regulatory T-cells (Tregs) (PubMed:25127859). Required during vasculogenesis (PubMed:12050137, PubMed:16251442).[UniProtKB/Swiss-Prot Function]