Nr1h3 (NM_013839) Mouse Tagged Lenti ORF Clone
CAT#: MR227522L4
- LentiORF®
Lenti ORF clone of Nr1h3 (mGFP-tagged) - Mouse nuclear receptor subfamily 1, group H, member 3 (Nr1h3), transcript variant 1
"NM_013839" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Nr1h3 |
Synonyms | AU018371; LXR; RLD1; Unr1 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR227522).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013839 |
ORF Size | 1335 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013839.4, NP_038867.2 |
RefSeq Size | 1955 bp |
RefSeq ORF | 1338 bp |
Locus ID | 22259 |
UniProt ID | Q9Z0Y9 |
Cytogenetics | 2 50.52 cM |
Gene Summary | Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:18055760, PubMed:19520913, PubMed:20427281). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215975 | Nr1h3 (untagged) - Mouse nuclear receptor subfamily 1, group H, member 3 (Nr1h3), transcript variant 1, (10ug) |
USD 503.00 |
|
MG227522 | Nr1h3 (tGFP-tagged) - Mouse nuclear receptor subfamily 1 group H member 3 (Nr1h3) transcript variant 1, (10ug) |
USD 657.00 |
|
MR227522 | Nr1h3 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 1, group H, member 3 (Nr1h3), transcript variant 1 |
USD 457.00 |
|
MR227522L3 | Lenti ORF clone of Nr1h3 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 1, group H, member 3 (Nr1h3), transcript variant 1 |
USD 757.00 |
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