Hdac5 (NM_001077696) Mouse Tagged ORF Clone

CAT#: MR227501

Hdac5 (Myc-DDK-tagged) - Mouse histone deacetylase 5 (Hdac5), transcript variant 1

Product Images

Circular map for MR227501

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hdac5
• Synonyms: AI426555; Hdac4; mHDA1; mKIAA0600
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227501 representing NM_001077696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCTCCCAACGAGTCGGCAGATGGCATGTCAGGCCGGGAACCATCCTTGGAAATCCTGCCACGGA
  CTCCTCTGCACAGCATCCCTGTGGCAGTGGAGGTGAAGCCGGTGCTGCCAGGAGCCATGCCCAGCTCCAT
  GGGGGGTGGAGGTGGAGGTAGCCCCAGCCCCGTGGAGCTTCGGGGGGCTCTGGCGGGCCCCATGGACCCT
  GCGCTACGGGAGCAGCAACTGCAGCAGGAGCTCCTGGTCCTCAAGCAGCAGCAGCAGCTCCAGAAGCAGC
  TCCTGTTCGCCGAGTTCCAGAAGCAGCACGACCACTTGACGCGGCAGCACGAGGTCCAGCTGCAGAAGCA
  CCTCAAGCAGCAGCAGGAGATGCTGGCGGCTAAGAGGCAGCAGGAGCTGGAGCAGCAGCGGCAGCGGGAG
  CAGCAGCGGCAGGAGGAGCTGGAGAAACAGCGGCTGGAGCAGCAGCTGCTCATCCTGCGCAACAAGGAGA
  AGAGCAAAGAGAGTGCCATCGCCAGCACCGAGGTAAAGCTGAGGCTCCAGGAATTCCTGTTGTCCAAGTC
  AAAGGAGCCCACGCCAGGCGGCCTCAACCATTCCCTCCCACAGCACCCCAAATGCTGGGGAGCCCACCAC
  GCTTCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCTCCTGGGACGCCTCCCTCCTACAAATTGCCTT
  TGCTTGGGCCCTATGACAGCCGTGATGACTTTCCCCTCCGTAAAACGGCCTCGGAACCCAACTTAAAAGT
  ACGTTCGAGGCTAAAACAGAAGGTAGCCGAGAGGAGAAGCAGTCCCCTCCTGCGTCGAAAGGATGGCACT
  GTTATTAGTACTTTTAAGAAGAGAGCAGTTGAGATCACCGGCACGGGGCCTGGGGTGTCGTCCGTGTGTA
  ACAGTGCGCCCGGCTCTGGCCCCAGCTCTCCCAACAGTTCCCACAGCACCATCGCTGAGAACGGCTTTAC
  TGGCTCAGTCCCCAACATCCCCACTGAGATGATCCCCCAGCACCGGGCCCTCCCTCTGGACAGTTCCCCA
  AACCAGTTCAGCCTCTATACGTCTCCTTCTCTGCCCAACATCTCCCTAGGGCTGCAGGCCACTGTCACTG
  TCACCAACTCGCACCTCACCGCCTCCCCGAAGCTGTCAACACAGCAGGAGGCTGAGAGGCAGGCCCTTCA
  GTCCCTGCGGCAGGGCGGCACACTGACCGGCAAGTTCATGAGCACATCCTCCATCCCTGGCTGCCTGTTG
  GGAGTGGCACTGGAGGGTGACACAAGCCCCCACGGGCACGCTTCCCTGCTGCAGCACGTTTTGCTCCTGG
  AGCAGGCCCGGCAACAGAGCACGCTCATAGCAGTGCCGCTCCATGGGCAGTCCCCACTGGTGACGGGTGA
  ACGTGTGGCCACCAGCATGAGGACGGTGGGTAAGCTCCCGAGGCACCGACCTCTGAGCCGCACTCAGTCC
  TCCCCGCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTCATGCAGCAGCAGCACCAGCAGTTCCTGG
  AGAAGCAGAAGCAGCAGCAGATGCAGCTGGGCAAGATCCTTACCAAAACTGGGGAGCTGTCAAGGCAGCC
  CACCACTCACCCGGAGGAGACAGAAGAGGAGCTGACGGAGCAGCAGGAGGCCTTGCTGGGAGAGGGGGCC
  CTGACCATTCCCCGGGAAGGCTCTACAGAAAGTGAGAGCACCCAGGAAGACCTAGAAGAGGAGGAGGAGG
  AGGAGGAGGAGGAAGAGGAGGACTGCATTCAGGTCAAGGATGAGGATGGCGAGAGTGGTCCTGATGAAGG
  CCCTGACTTAGAAGAGTCCAGTGCTGGTTACAAAAAGTTGTTCGCAGATGCCCAGCAGTTACAGCCCCTC
  CAGGTGTACCAGGCACCCCTCAGCCTGGCCACTGTGCCTCATCAGGCCCTGGGCCGCACCCAGTCCTCAC
  CTGCTGCTCCTGGGAGCATGAAGAGCCCCACAGACCAACCCACTGTGGTGAAGCACCTCTTCACCACAGG
  TGTGGTCTATGACACGTTCATGCTGAAGCACCAGTGTATGTGCGGAAACACACACGTGCACCCAGAGCAC
  GCCGGCCGCATCCAGAGCATCTGGTCCCGGCTGCAGGAAACTGGTCTGCTCGGCAAGTGTGAGCGGATCC
  GGGGTCGTAAAGCCACACTGGATGAAATCCAGACCGTGCACTCTGAGTACCACACCCTGCTCTATGGGAC
  CAGCCCCCTTAACCGGCAGAAGCTGGACAGCAAGAAGCTGCTTGGCCCCATCAGCCAGAAGATGTACGCC
  ATGCTGCCCTGTGGGGGCATTGGGGTGGACAGTGACACGGTGTGGAATGAGATGCACTCCTCAAGTGCCG
  TGCGAATGGCAGTGGGCTGCCTGGTGGAGCTGGCCTTCAAGGTGGCTGCAGGAGAGCTCAAGAATGGATT
  TGCTATCATCCGGCCCCCAGGACACCATGCTGAGGAGTCCACAGCCATGGGATTCTGCTTCTTCAACTCC
  GTAGCCATCACAGCTAAACTCCTGCAGCAGAAGCTGAGCGTGGGCAAGGTCCTCATCGTGGACTGGGATA
  TTCACCATGGCAACGGCACCCAGCAAGCATTCTACAACGATCCCTCTGTGCTCTACATCTCCCTGCATCG
  CTACGACAACGGGAACTTCTTTCCAGGCTCTGGGGCTCCTGAAGAGGTTGGTGGAGGGCCAGGTGTGGGG
  TACAACGTAAATGTGGCGTGGACAGGAGGTGTGGATCCCCCCATTGGAGATGTGGAATACCTGACAGCCT
  TCAGGACAGTGGTGATGCCCATTGCCCAGGAGTTCTCACCTGACGTCGTCCTAGTCTCCGCTGGGTTTGA
  TGCTGTTGAAGGACATCTGTCTCCACTGGGTGGCTATTCTGTCACCGCCAGATGTTTTGGCCACTTGACC
  AGGCAGCTCATGACACTGGCTGGGGGCCGGGTGGTGCTGGCCCTGGAGGGAGGCCATGACTTGACCGCCA
  TCTGTGATGCCTCTGAGGCCTGTGTCTCGGCTCTGCTCAGCGTGGAGCTGCAGCCCTTGGATGAAGCAGT
  CTTGCAGCAAAAGCCCAGCGTCAATGCGGTTGCCACACTAGAGAAAGTCATCGAGATCCAGAGCAAACAC
  TGGAGCTGTGTACAGAGGTTTGCCGCTGGTCTGGGCTGCTCGCTGCGGGAGGCTCAGACAGGTGAGAAAG
  AGGAGGCCGAGACTGTGAGCGCCATGGCCCTGCTTTCCGTGGGGGCTGAGCAGGCCCAGGCTGTTGCCAC
  TCAAGAGCACAGCCCCAGGCCAGCAGAGGAGCCCATGGAGCAGGAGCCTGCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227501 representing NM_001077696
  Red=Cloning site Green=Tags(s)
  
  MNSPNESADGMSGREPSLEILPRTPLHSIPVAVEVKPVLPGAMPSSMGGGGGGSPSPVELRGALAGPMDP
  ALREQQLQQELLVLKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKRQQELEQQRQRE
  QQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHH
  ASLDQSSPPQSGPPGTPPSYKLPLLGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGT
  VISTFKKRAVEITGTGPGVSSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMIPQHRALPLDSSP
  NQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLL
  GVALEGDTSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQS
  SPLPQSPQALQQLVMQQQHQQFLEKQKQQQMQLGKILTKTGELSRQPTTHPEETEEELTEQQEALLGEGA
  LTIPREGSTESESTQEDLEEEEEEEEEEEEDCIQVKDEDGESGPDEGPDLEESSAGYKKLFADAQQLQPL
  QVYQAPLSLATVPHQALGRTQSSPAAPGSMKSPTDQPTVVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEH
  AGRIQSIWSRLQETGLLGKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYA
  MLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLVELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNS
  VAITAKLLQQKLSVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVG
  YNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAQEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLT
  RQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPSVNAVATLEKVIEIQSKH
  WSCVQRFAAGLGCSLREAQTGEKEEAETVSAMALLSVGAEQAQAVATQEHSPRPAEEPMEQEPAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077696
• ORF Size: 3345 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077696.1, NP_001071164.1
• RefSeq Size: 3816 bp
• RefSeq ORF: 3348 bp
• Locus ID: 15184
• Cytogenetics: 11 D
• MW: 121.2 kDa