Slc11a1 (NM_013612) Mouse Tagged ORF Clone

CAT#: MR227222

Slc11a1 (Myc-DDK-tagged) - Mouse solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (Slc11a1)

Product Images

Circular map for MR227222

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc11a1
• Synonyms: Bcg; Ity; ity; Ity1; Lsh; Nramp; Nramp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227222 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTAGTGACAAGAGCCCCCCGAGGCTGAGCAGGCCCAGTTATGGCTCCATTTCCAGCCTGCCTGGCC
  CAGCACCTCAGCCAGCGCCTTGCCGGGAGACCTACCTGAGTGAGAAGATCCCCATTCCCAGCGCAGACCA
  GGGTACATTCAGCCTGAGGAAGCTGTGGGCGTTCACGGGGCCTGGTTTCCTCATGAGCATCGCTTTCCTT
  GACCCGGGAAACATTGAGTCCGACCTTCAAGCTGGCGCTGTGGCTGGGTTCAAACTCCTCTGGGTGCTGC
  TCTGGGCGACTGTGCTAGGTTTGCTGTGCCAGCGGCTGGCTGCCCGGCTGGGCGTGGTGACAGGCAAGGA
  CTTGGGTGAAGTCTGCCATCTCTACTACCCCAAGGTGCCCCGCATCCTCCTCTGGCTGACCATTGAGCTG
  GCCATTGTGGGCTCAGATATGCAGGAAGTCATCGGGACGGCTATCTCCTTCAATCTGCTCTCCGCTGGAC
  GCATCCCGCTGTGGGACGGTGTACTGATCACCATTGTGGACACCTTCTTCTTCCTCTTCTTGGATAACTA
  TGGTTTGCGCAAGCTGGAAGCTTTCTTCGGTCTCCTCATTACCATAATGGCTTTGACCTTCGGCTATGAG
  TATGTGGTAGCACACCCTTCCCAGGGAGCGCTCCTTAAGGGCCTGGTGCTGCCCACCTGTCCGGGCTGTG
  GGCAGCCCGAGCTGCTGCAGGCAGTGGGCATCGTCGGTGCCATCATCATGCCCCATAACATCTACCTGCA
  CTCAGCCTTGGTCAAGTCTAGAGAAGTAGACAGAACCCGCCGGGTGGATGTTCGAGAAGCCAACATGTAC
  TTCCTGATTGAGGCCACCATCGCCCTATCGGTGTCCTTCATCATCAACCTCTTCGTCATGGCTGTTTTTG
  GTCAGGCCTTCTACCAGCAAACCAATGAGGAAGCGTTCAACATCTGTGCCAACAGCAGCCTCCAGAACTA
  TGCTAAGATCTTCCCCAGGGACAATAACACTGTGTCAGTGGATATTTATCAAGGAGGTGTGATCCTAGGC
  TGTCTCTTTGGCCCTGCGGCCCTCTACATCTGGGCAGTAGGTCTCCTGGCAGCGGGGCAGAGTTCTACTA
  TGACCGGCACCTATGCAGGACAGTTCGTGATGGAGGGTTTCCTTAAGCTGCGGTGGTCCCGCTTCGCTCG
  GGTCCTTCTCACGCGCTCTTGCGCCATCCTGCCCACTGTGTTGGTGGCTGTCTTCCGAGACCTGAAGGAC
  CTGTCCGGCCTCAACGATCTACTCAATGTTCTGCAGAGTCTACTGCTGCCCTTCGCTGTACTGCCCATTT
  TGACTTTCACCAGCATGCCAGCTGTCATGCAGGAGTTTGCCAACGGCCGGATGAGCAAAGCCATCACTTC
  GTGCATCATGGCGCTAGTCTGCGCCATCAACCTGTACTTTGTGATCAGCTACCTGCCCAGCCTCCCGCAC
  CCTGCCTACTTTGGCCTTGTGGCTCTGTTCGCAATAGGTTACTTGGGCCTGACTGCTTATCTGGCCTGGA
  CCTGTTGCATCGCCCACGGAGCCACCTTCCTGACCCACAGCTCCCACAAGCACTTCTTATATGGGCTCCC
  TAACGAGGAGCAGGGAGGCGTGCAGGGTTCCGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227222 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MISDKSPPRLSRPSYGSISSLPGPAPQPAPCRETYLSEKIPIPSADQGTFSLRKLWAFTGPGFLMSIAFL
  DPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRILLWLTIEL
  AIVGSDMQEVIGTAISFNLLSAGRIPLWDGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTFGYE
  YVVAHPSQGALLKGLVLPTCPGCGQPELLQAVGIVGAIIMPHNIYLHSALVKSREVDRTRRVDVREANMY
  FLIEATIALSVSFIINLFVMAVFGQAFYQQTNEEAFNICANSSLQNYAKIFPRDNNTVSVDIYQGGVILG
  CLFGPAALYIWAVGLLAAGQSSTMTGTYAGQFVMEGFLKLRWSRFARVLLTRSCAILPTVLVAVFRDLKD
  LSGLNDLLNVLQSLLLPFAVLPILTFTSMPAVMQEFANGRMSKAITSCIMALVCAINLYFVISYLPSLPH
  PAYFGLVALFAIGYLGLTAYLAWTCCIAHGATFLTHSSHKHFLYGLPNEEQGGVQGSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013612
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013612.2, NP_038640.2
• RefSeq Size: 2304 bp
• RefSeq ORF: 1647 bp
• Locus ID: 18173
• UniProt ID: P41251
• Cytogenetics: 1 38.54 cM
• MW: 59.7 kDa
• Gene Summary: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.[UniProtKB/Swiss-Prot Function]