Map3k5 (NM_008580) Mouse Tagged ORF Clone

CAT#: MR227032

Map3k5 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 5 (Map3k5)

Product Images

Circular map for MR227032

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map3k5
• Synonyms: 7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227032 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGAAGCCGGCGAGGGCATCACTTTCTCCGTGCCGCCCTTTGCGTCCGTGGGCTTTTGCACCA
  TCCCGGAGGGCGGCAGCTGCCGGAGGGGAGGAGGAGCGGCGACGGCGGCGGAGGGAGAGCCGAGCCTGCA
  GCCCTTGCTTGTGCCGCCGCCGCCGCCACCGCCGGGCAGTTTCTGGAACGTGGAGAGCGCGGCGGCTCCC
  GGCACCAGCTGCCCTACCACCGCCCCCGGGAGCAGCGCCACCCGAGGCCGGGGCAACTCTGGCAGCGGGG
  GCGGCCGGCGTACCACCGTGGCGTATGTGATCAATGAGGCGAGCCAGGGGCAGCTGGTGGTGGCGGAGAG
  CGAGGCCCTGCAGAGCCTGCGGGAGGCGTGCGAGGCGGTGGGCGCCACCCTGGAGACCCTGCATTTCGGG
  AAGCTGGACTTCGGGGAAACCGCCGTGCTGGACCGTTTTTACAACGCAGATATTGCTGTGGTGGAGATGA
  GCGACGCCTTCCGGCAGCCTTCCTTGTTTTACCATCTTGGAGTGCGAGAAAGCTTCAGTATGGCCAACAA
  CATCATCCTCTACTGCGATACTAATTCCGATTCACTCCAGTCCCTGAAGGAAATTATTTGCCAGAAGAAT
  ACTGTGTGCACCGGGAACTACACCTTCATCCCCTACATGGTGACACCACACAACAAGGTCTACTGCTGTG
  ACAGCAGCTTCATGAAGGGCCTGACTGAGCTCATGCAGCCTAACTTCGAGCTCCTGCTGGGGCCCATCTG
  CTTACCCCTCGTGGACCGTTTTGTTCAGCTTTTGAAGGTGGCGCAAGCGAGTTCCAGCCAGTACTTCCGG
  GAATCCATACTCAGCGATATCAGGAAAGCACGGAACCTGTACACTGGTAAAGAATTGGCAGCTGAATTGG
  CGAGGATCCGGCAACGGGTAGACAATATTGAAGTTTTGACAGCAGACATTGTTATCAACCTGTTGCTTTC
  CTACAGAGACATCCAGGACTATGACTCCATAGTGAAGCTGGTAGAGACGTTAGAAAAACTTCCAACCTTC
  GACTTGGCCTCGCACCACCACGTGAAGTTTCATTACGCATTTGCACTGAACAGGAGAAATCTCCCTGGAG
  ACAGAGCAAAAGCTCTTGACATCATGATCCCCATGGTACAAAGTGAAGAGCAAGTTGCTTCGGATATGTA
  TTGTCTCGTTGGCCGAATCTACAAAGATATGTTTTTGGATTCAAATTTCACTGACACGGAAAGCAGGGAC
  CATGGAGCATCTTGGTTCAAAAAGGCATTTGAGTCTGAGCCAACACTACAGTCAGGAATTAATTATGCAG
  TTCTCTTGCTGGCGGCTGGGCACCAGTTTGAATCTTCTTTTGAGCTTCGGAAAGTTGGAGTGAAGCTGAG
  TAGCCTTCTGGGTAAAAAGGGGAACTTGGAAAAACTCCAGAGCTACTGGGAAGTCGGATTTTTTCTGGGA
  GCCAGTGTCCTGGCCAACGACCACCTCAGGGTCATTCAGGCATCCGAGAAGCTGTTCAGACTGAAGACAC
  CAGCGTGGTACCTCAAGTCTATCGTGGAGACCATTCTGATATATAAACACTTTGTGAAACTGACCACAGA
  GCAGCCCTCGGCTAAGCAGGAACTCGTGGACTTCTGGATGGATTTCCTGGTCGAGGCCACAAAAACAGAT
  GTTACTGTTGTCAGGTTCCCAGTATTAATATTAGAGCCAACCAAAATCTATCAGCCTTCTTACCTGTCTA
  TCAATAATGAAGTTGAGGAGAAGACAATATCTATTTGGCATGTTCTCCCTGATGACAAGAAAGGTATACA
  CGAGTGGAACTTCGGTGCTTCCTCTGTCAGGGGAGTGAGCATCTCTAAGTTTGAAGAACGATGCTGTTTC
  CTTTATGTGCTTCACAATTCTGATGACTTCCAAATCTACTTCTGCACAGAACTTCACTGTAAAAGGTTTT
  TTGAAATGGTGAATACCATCACGGAAGAGAAGGGGAGAGGCGCGGAGGACGGAGACTGTGAGGGTGACTC
  CCTGGAGTATGACTACGAATACGATGAAAATGGAGACAGAGTTGTGTTAGGGAAGGGCACTTATGGGATC
  GTCTATGCAGGACGAGACCTGAGCAACCAGGTCAGAATTGCTATTAAGGAAATCCCAGAGAGAGACAGCA
  GATACTCTCAGCCCCTGCATGAAGAAATCGCCCTGCACAAGCATCTGAAACACAAAAACATTGTCCAGTA
  TCTGGGCTCCTTCAGTGAGAACGGCTTCATCAAAATCTTCATGGAGCAAGTCCCAGGAGGAAGCCTCTCT
  GCTCTCCTTCGTTCAAAATGGGGCCCATTAAAGGACAACGAACAGACGATTGGCTTCTATACGAAGCAGA
  TACTGGAAGGATTAAAATACCTTCACGACAATCAGATAGTCCACCGGGATATAAAGGGTGACAATGTATT
  GATTAATACCTACAGTGGTGTGCTCAAGATCTCTGACTTCGGGACATCCAAGAGGCTTGCTGGCATAAAC
  CCATGTACCGAAACCTTTACAGGTACCCTTCAGTATATGGCGCCAGAAATAATCGATAAAGGACCACGAG
  GCTATGGAAAGGCCGCCGACATTTGGTCACTGGGCTGCACAATCATCGAAATGGCTACTGGAAAACCACC
  ATTTTATGAACTAGGAGAGCCACAGGCAGCCATGTTCAAGGTGGGGATGTTTAAGGTCCACCCGGAGATC
  CCAGAGTCCATGTCGGCGGAGGCCAAGGCGTTCATACTGAAGTGTTTTGAACCAGACCCTGACAAGAGAG
  CCTGTGCTAATGACTTGCTTATTGATGAGTTCTTAAAAGTGTCCAGCAAAAAGAAAAAGACACAACCCAA
  ACTTTCAGCTCTCTCGACTGGATCGAATGAGTATCTTAGAAGCATCTCCCTGCCGGTCCCTGTCCTGGTA
  GAAGACACCAGCAGCAGTAGCGAGTATGGCTCTGTTTCTCCTGATACAGAGCTGAAGGCGGACCCCTTCT
  CCTTCAAAGCCAGAGCTAAGTCCTGTGGAGAAAAGGACGGGAAAGGGATACGGACATTGTTCCTGGGCAT
  TCCAGATGAAAATTTTGAAGATCACAGTGCGCCCCCATCTCCTGAAGAGAAAGACTCGGGCTTTTTTATG
  CTGAGAAAGGATAGTGAGAGGCGAGCTACCCTTCACAGAATCCTGACCGAAGACCAGGACAAGGTTGTAA
  GGAACCTAATGGAATCTCTGGCCCAGGGTGCTGAAGAGCCTAAACTAAAGTGGGAACACATCACAACCCT
  CATCTCAAGCCTCAGAGAGTTTGTGAGGTCCACTGACCGAAAAATCATAGCCACTACATTGTCCAAGCTC
  AAGCTGGAGCTGGACTTCGACAGCCACGGCATCAGCCAGGTGCAGGTGGTCCTCTTTGGCTTCCAGGATG
  CGGTCAATAAAGTTCTTCGGAATCATAACATCAAGCCACACTGGATGTTTGCCCTGGACAGCATCATCCG
  AAAGGCTGTGCAGACTGCAATTACCATTCTGGTTCCAGAGTTGAGGCCACATTTTAGCCTTGCATCAGAG
  AGCGACACAGCTGATCCGGAAGACCTGGATGTCGAAGATGAACACGAGGAATTGTCTTCAAATCAGACAG
  TCCGACGGCCTCAGGCCATCACTGAGGACGCTGTGGCTACCTCGGGGGTGAGCACGCTAAGCTCCACTGT
  GTCCCATGACTCCCAGAATGCACACCGGTCGCTGAACGTGCAGCTTGGAAGGATGAAGATTGAAACTAAT
  AGGTTACTTGAAGAGCTGGTTCGGAAAGAGAGAGAGTTACAGGCACTCCTCCATCAAGCCATTGAGGAAA
  AAGACCAAGAAATTAGGCACCTGAAGCTCAAGTCCCAGCCCATAGATATCCCTGGGTTTCCTGTGTGCCA
  CCTGAACTCTCCTGGCACGACCACGGAAGATTCTGAACTTCCTGGCTGGCTGAGAGAAAATGGAGCTGAT
  GAAGACACTATAAGTCGGTTTCTGGCTGAGGATTACACGCTAGTGGATGTTCTTTACTACGTCACACGTG
  ATGATCTGAAGTGCCTCAGACTAAGGGGCGGGATGCTGTGCACGCTGTGGAAGGCCATCATTGACTTTCG
  GAACAAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227032 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGTEAGEGITFSVPPFASVGFCTIPEGGSCRRGGGAATAAEGEPSLQPLLVPPPPPPPGSFWNVESAAAP
  GTSCPTTAPGSSATRGRGNSGSGGGRRTTVAYVINEASQGQLVVAESEALQSLREACEAVGATLETLHFG
  KLDFGETAVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKN
  TVCTGNYTFIPYMVTPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFVQLLKVAQASSSQYFR
  ESILSDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTF
  DLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEEQVASDMYCLVGRIYKDMFLDSNFTDTESRD
  HGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLG
  ASVLANDHLRVIQASEKLFRLKTPAWYLKSIVETILIYKHFVKLTTEQPSAKQELVDFWMDFLVEATKTD
  VTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFGASSVRGVSISKFEERCCF
  LYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEDGDCEGDSLEYDYEYDENGDRVVLGKGTYGI
  VYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLS
  ALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGIN
  PCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEI
  PESMSAEAKAFILKCFEPDPDKRACANDLLIDEFLKVSSKKKKTQPKLSALSTGSNEYLRSISLPVPVLV
  EDTSSSSEYGSVSPDTELKADPFSFKARAKSCGEKDGKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFM
  LRKDSERRATLHRILTEDQDKVVRNLMESLAQGAEEPKLKWEHITTLISSLREFVRSTDRKIIATTLSKL
  KLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASE
  SDTADPEDLDVEDEHEELSSNQTVRRPQAITEDAVATSGVSTLSSTVSHDSQNAHRSLNVQLGRMKIETN
  RLLEELVRKERELQALLHQAIEEKDQEIRHLKLKSQPIDIPGFPVCHLNSPGTTTEDSELPGWLRENGAD
  EDTISRFLAEDYTLVDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008580
• ORF Size: 4143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008580.3, NM_008580.4, NP_032606.4
• RefSeq Size: 5393 bp
• RefSeq ORF: 4143 bp
• Locus ID: 26408
• UniProt ID: O35099
• Cytogenetics: 10 A3
• MW: 154.5 kDa
• Gene Summary: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).[UniProtKB/Swiss-Prot Function]