Sfn (NM_018754) Mouse Tagged ORF Clone
CAT#: MR227029
- TrueORF®
Sfn (Myc-DDK-tagged) - Mouse stratifin (Sfn)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_018754" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Sfn |
Synonyms | Er; Mme1; Ywhas |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227029 representing NM_018754
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGAGCCAGTCTGATCCAGAAGGCCAAGTTGGCTGAACAGGCCGAACGGTATGAAGACATGGCAG CTTTCATGAAGAGCGCCGTGGAAAAGGGCGAGGAGCTCTCCTGCGAGGAGCGAAACCTGCTTTCCGTAGC TTACAAGAACGTGGTGGGCGGCCAGAGAGCGGCCTGGAGGGTCCTGTCCAGCATCGAGCAGAAGAGCAAC GAGGAGGGGTCAGAAGAGAAGGGCCCCGAGGTGAAAGAGTACCGGGAGAAGGTAGAGACCGAGCTCAGAG GTGTGTGCGACACCGTACTCGGCCTGCTGGACTCGCACCTCATCAAAGGGGCTGGAGATGCAGAGAGCCG CGTCTTCTACCTGAAGATGAAGGGTGACTACTACCGCTACCTAGCCGAGGTGGCCACTGGCGATGACAAG AAGCGCATCATCGATTCTGCCCGGTCAGCCTACCAGGAGGCCATGGACATCAGCAAGAAGGAGATGCCGC CTACCAACCCCATCCGCCTGGGCCTGGCCCTGAACTTTTCAGTCTTCCACTACGAGATAGCCAACAGCCC CGAGGAGGCCATCTCGCTGGCCAAGACCACCTTCGACGAGGCCATGGCCGACCTGCACACCCTCAGTGAG GACTCCTACAAGGACAGCACCCTCATCATGCAGCTCCTGAGAGACAACCTGACGCTGTGGACAGCCGACA GTGCTGGGGAAGAGGGTGGTGAGGCTCCGGAGGAGCCCCAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227029 representing NM_018754
Red=Cloning site Green=Tags(s) MERASLIQKAKLAEQAERYEDMAAFMKSAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSN EEGSEEKGPEVKEYREKVETELRGVCDTVLGLLDSHLIKGAGDAESRVFYLKMKGDYYRYLAEVATGDDK KRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSE DSYKDSTLIMQLLRDNLTLWTADSAGEEGGEAPEEPQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018754 |
ORF Size | 744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018754.2, NP_061224.2 |
RefSeq Size | 1616 bp |
RefSeq ORF | 747 bp |
Locus ID | 55948 |
UniProt ID | O70456 |
Cytogenetics | 4 66.25 cM |
MW | 28.2 kDa |
Gene Summary | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209999 | Sfn (untagged) - Mouse stratifin (Sfn), (10ug) |
USD 330.00 |
|
MG227029 | Sfn (tGFP-tagged) - Mouse stratifin (Sfn), (10ug) |
USD 500.00 |
|
MR227029L3 | Lenti ORF clone of Sfn (Myc-DDK-tagged) - Mouse stratifin (Sfn) |
USD 600.00 |
|
MR227029L4 | Lenti ORF clone of Sfn (mGFP-tagged) - Mouse stratifin (Sfn) |
USD 600.00 |
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