Myh4 (NM_010855) Mouse Tagged ORF Clone

CAT#: MR226919

Myh4 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 4, skeletal muscle (Myh4)

Product Images

Circular map for MR226919

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myh4
• Synonyms: AI506973; MHC2B; Minimsc; Minmus; MM; MYH-2B; MyHC-IIb; Myhsf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226919 representing NM_010855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCCGACGCTGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC
  GAATCGAGGCCCAGAACAAGCCTTTTGATGCCAAGTCATCGGTGTTTGTGGTGGATGCTAAGGAGTCCTA
  TGTGAAAGCAACAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGGCGGAGCTACGGTC
  ACCGTCAAGGATGATCAAGTCTTCTCCATGAACCCTCCCAAGTACGACAAGATTGAGGACATGGCCATGA
  TGACCCACCTGCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC
  TTACTCAGGCCTCTTTTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG
  GCAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGATAACGCCTACC
  AGTTCATGCTGACGGATCGTGAGAATCAGTCCATCCTGATTACTGGAGAATCCGGGGCCGGGAAGACTGT
  GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAGGAGGCG
  ACTTCTGGCAAAATGCAGGGGACCCTTGAAGATCAAATTATCAGTGCCAACCCCCTGCTGGAGGCCTTCG
  GCAACGCCAAGACTGTGAGGAACGACAACTCTTCTCGATTTGGTAAATTCATCAGAATCCATTTCGGTGC
  CACAGGCAAACTGGCCTCTGCAGATATTGAAACCTATCTGCTAGAGAAGTCCAGAGTCACCTTCCAGCTC
  AAGGCTGAAAGGAGCTACCACATCTTCTACCAAATCATGTCCAATAAGAAACCAGAGCTTATTGAGATGC
  TTCTGATCACCACCAACCCATATGACTTTGCTTACGTCAGTCAAGGTGAAATCACAGTGCCCAGCATTGA
  TGACCAGGAAGAGCTGATGGCCACAGACACTGCTGTTGATATCCTGGGATTCAGTGCAGATGAAAAGGTG
  GCCATTTACAAGCTCACAGGCGCTGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAAAGGGAAG
  AGCAGGCTGAGCCAGACGGCACCGAAGTTGCTGACAAGGCTGCCTATCTGACAAGTCTGAACTCTGCTGA
  CCTGCTCAAAGCCCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACGGTG
  CAGCAGGTGTACAACTCCGTGGGTGCCCTGGCCAAGTCCATGTATGAGAAGATGTTCCTATGGATGGTCA
  CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG
  CTTTGAGATCTTTGACTTCAACACCTTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG
  TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGATTGGGAGTTCA
  TTGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT
  GGAAGAGGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAGCAGCATCTT
  GGAAAGTCCAACAACTTCCAGAAGCCCAAACCCGCCAAAGGCAAGGCTGAGGCTCACTTCTCCCTGGTGC
  ACTATGCGGGCACCGTGGACTACAACATTATTGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC
  CGTGGTGGGGCTGTACCAGAAATCCGGGTTGAAGACTCTGGCTTTCCTATTTTCTGGGGGACAAGCTGCG
  GAAGCAGAGGGCGGCGGTGGAAAGAAAGGTGGCAAGAAGAAGGGTTCTTCTTTCCAGACCGTGTCAGCTC
  TCTTCAGGGAGAATTTAAATAAGCTGATGACCAACTTGAAGAGCACCCACCCCCACTTTGTCAGATGCCT
  CATTCCCAATGAAACTAAGACTCCTGGTGCCATGGAGCATGAACTGGTCTTGCACCAGCTCAGGTGTAAC
  GGGGTGCTGGAAGGCATCCGCATCTGTAGGAAGGGGTTCCCCAGCAGGATCCTCTATGCAGACTTCAAGC
  AGAGATACAAAGTCCTGAATGCAAGTGCCATCCCTGAGGGTCAGTTCATTGATAGCAAGAAGGCCTCTGA
  GAAACTTCTGGGTTCCATTGACATTGACCACACTCAGTACAAATTCGGTCACACTAAGGTTTTCTTTAAA
  GCCGGCCTGTTGGGAACTCTAGAGGAGATGCGAGATGAAAAGCTGGCTCAGCTCATCACCCGCACTCAAG
  CCGTGTGCAGAGGGTACCTGATGAGGGTGGAGTTCAAAAAGATGATGGAGAGGAGAGAGTCCATCTTCTG
  CATTCAGTACAACGTCCGCGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTATTTCAAGATC
  AAACCTCTGCTGAAGAGCGCGGAGACCGAGAAGGAGATGGCCAACATGAAGGAAGATTTTGAGAAGGCCA
  AGGAGGATCTGGCTAAGTCAGAGGCAAAAAGGAAGGAACTAGAAGAAAAGATGGTAGCTTTGATGCAGGA
  GAAAAATGACCTGCAGCTCCAGGTTCAAGCTGAAGCAGATGGCTTGGCTGATGCTGAGGAAAGGTGCGAC
  CAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGCTGACCGAGAGGGCCGAGGACGAGG
  AGGAGATCAACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAGGA
  CATTGACGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTGAAA
  AACCTGACAGAAGAGATGGCAGGCCTGGATGAAAATATCGCCAAGCTGACCAAGGAGAAGAAGGCCCTCC
  AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGC
  CAAAACCAAGCTGGAACAGCAAGTGGATGACCTTGAAGGATCACTGGAACAGGAAAAGAAGCTGAGGATG
  GACTTAGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAG
  AAAACGATAAACAGCAGCTCGATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCTGCAGAGCAA
  GATTGAGGACGAGCAGGCCCTCGGCATGCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCCCGCATCGAG
  GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCTAAAGCAGAGAAGCAGCGCTCTGACCTCT
  CCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCTGGCGGAGCCACTTCAGCCCAGATCGAGAT
  GAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTGCAGCATGAA
  GCCACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGATCGACAACC
  TGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGATCTCGCTAG
  TAACATGGAGACAGTCTCCAAAGCCAAGGGGAACCTTGAGAAGATGTGCCGCACCCTGGAGGACCAACTG
  AGTGAAGTGAAGACCAAGGAGGAGGAGCAGCAGCGCCTGATCAATGAACTGTCAACCCAGAAGGCACGCT
  TGCACACAGAGTCAGGCGAGTTTTCCCGACAGCTGGATGAAAAAGACGCTATGGTTTCTCAGCTTTCCCG
  AGGCAAACAAGCATTTACACAACAGATTGAGGAACTAAAGAGGCAGCTAGAGGAAGAGTCCAAGGCCAAG
  AACGCACTGGCCCACGCCCTGCAGTCAGCTCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAAGAGG
  AGCAGGAAGCCAAGGCTGAGCTGCAGAGGGCCATGTCCAAGGCCAACAGCGAGGTAGCCCAGTGGAGGAC
  GAAATATGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAAGAGGCCAAGAAGAAGCTGGCTCAGCGT
  CTGCAGGATGCTGAGGAACATGTAGAAGCTGTGAATTCCAAATGCGCTTCTCTTGAAAAGACGAAGCAGC
  GGCTTCAGAATGAAGTGGAGGACCTCATGATTGACGTGGAGAGGTCTAACGCTGCCTGCGCTGCGCTTGA
  TAAGAAGCAGAGGAACTTTGACAAGGTCCTGGCAGAATGGAAACAGAAGTACGAGGAAACTCAGGCTGAG
  CTTGAAGCCTCCCAGAAGGAGTCCCGCTCTCTGAGCACTGAGCTGTTCAAAGTGAAGAATGCCTATGAGG
  AGTCTCTGGATCAACTTGAGACGTTAAAGAGAGAGAACAAGAACCTGCAGCAGGAGATCTCTGACCTGAC
  CGAGCAGATTGCTGAGGGAGGGAAGCACATCCATGAGCTGGAGAAAATCAAGAAGCAAATTGATCAGGAA
  AAGAGTGAATTACAGGCTTCTCTGGAGGAAGCGGAGGCATCCCTTGAACATGAAGAAGGTAAAATCCTAC
  GCATCCAGCTGGAGTTGAACCAGGTGAAATCTGAGATTGACCGCAAAATTGCTGAAAAAGATGAAGAGAT
  AGATCAGCTGAAGAGAAACCACCTCAGAGTTGTGGAGTCCATGCAGAGCACGCTGGACGCCGAGATCCGC
  AGCAGGAATGATGCTCTGAGGATTAAGAAGAAGATGGAGGGAGACCTCAATGAGATGGAGATCCAGCTGA
  ACCATGCCAACCGCCAGGCTGCGGAGGCAATCAGGAACCTTCGGAACACCCAGGGAATGCTGAAGGACAC
  ACAGCTGCACCTGGACGATGCTCTCAGAGGCCAGGACGACCTGAAAGAGCAGCTGGCCATGGTTGAGCGC
  AGAGCCAACCTGATGCAGGCTGAGATCGAGGAGCTCAGGGCATCCCTGGAACAGACAGAGAGGAGCAGGA
  GAGTGGCCGAGCAAGAGCTACTGGATGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAACACCAGCCT
  CATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAAGACATCGTCCAG
  GAAGCCCGCAACGCAGAAGAGAAGGCCAAGAAAGCCATCACTGATGCCGCCATGATGGCGGAGGAGCTGA
  AGAAGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGACCT
  GCAGCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAG
  GCTAGGGTGAGGGAGCTTGAAAACGAGGTGGAAAATGAACAGAAGCGCAACATCGAAGCCGTCAAGGGTC
  TTCGTAAGCACGAGCGCAGAGTGAAGGAACTCACCTACCAGACCGAGGAGGACCGCAAGAACGTGCTGAG
  GCTGCAGGACTTGGTGGACAAACTACAGACTAAAGTGAAAGCCTACAAGAGACAGGCTGAGGAGGCTGAG
  GAACAATCCAATGTCAACCTGGCCAAGTTCCGTAAGATCCAGCACGAGCTGGAGGAAGCCGAGGAGCGGG
  CTGACATCGCGGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGAGAGGTTCACACTAAAGTCATAAG
  CGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226919 representing NM_010855
  Red=Cloning site Green=Tags(s)
  
  MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESYVKATVQSREGGKVTAKTEGGATV
  TVKDDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV
  AAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEEA
  TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQL
  KAERSYHIFYQIMSNKKPELIEMLLITTNPYDFAYVSQGEITVPSIDDQEELMATDTAVDILGFSADEKV
  AIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKALCYPRVKVGNEYVTKGQTV
  QQVYNSVGALAKSMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNTLEQLCINFTNEKLQQ
  FFNHHMFVLEQEEYKKEGIDWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL
  GKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNIIGWLDKNKDPLNETVVGLYQKSGLKTLAFLFSGGQAA
  EAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLKSTHPHFVRCLIPNETKTPGAMEHELVLHQLRCN
  GVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFK
  AGLLGTLEEMRDEKLAQLITRTQAVCRGYLMRVEFKKMMERRESIFCIQYNVRAFMNVKHWPWMKLYFKI
  KPLLKSAETEKEMANMKEDFEKAKEDLAKSEAKRKELEEKMVALMQEKNDLQLQVQAEADGLADAEERCD
  QLIKTKIQLEAKIKELTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVK
  NLTEEMAGLDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRM
  DLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIE
  ELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHE
  ATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMETVSKAKGNLEKMCRTLEDQL
  SEVKTKEEEQQRLINELSTQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEESKAK
  NALAHALQSARHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQR
  LQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACAALDKKQRNFDKVLAEWKQKYEETQAE
  LEASQKESRSLSTELFKVKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQIDQE
  KSELQASLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRVVESMQSTLDAEIR
  SRNDALRIKKKMEGDLNEMEIQLNHANRQAAEAIRNLRNTQGMLKDTQLHLDDALRGQDDLKEQLAMVER
  RANLMQAEIEELRASLEQTERSRRVAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQ
  EARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLE
  ARVRELENEVENEQKRNIEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQTKVKAYKRQAEEAE
  EQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010855
• ORF Size: 5820 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 6037 bp
• RefSeq ORF: 5820 bp
• Locus ID: 17884
• UniProt ID: Q5SX39
• Cytogenetics: 11 40.59 cM
• MW: 222.9 kDa
• Gene Summary: Muscle contraction.[UniProtKB/Swiss-Prot Function]