Cntn1 (NM_001159647) Mouse Tagged ORF Clone

CAT#: MR226639

Cntn1 (Myc-DDK-tagged) - Mouse contactin 1 (Cntn1), transcript variant 1

Product Images

Circular map for MR226639

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cntn1
• Synonyms: AW495098; CNTN; F3cam; usl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226639 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGCCATTGCTGGTCAGCCATCTCCTGCTAATCTCCTTAACATCTTGTCTAGGAGACTTTACCT
  GGCACAGAAGATATGGTCATGGAGTGTCTGAGGAGGACAAAGGATTTGGGCCCATTTTTGAAGAGCAGCC
  AATCAATACCATTTACCCAGAAGAGTCACTGGAAGGAAAAGTGTCACTCAACTGCCGGGCACGCGCCAGC
  CCATTTCCAGTTTACAAATGGAGAATGAATAACGGGGACGTGGACCTGACAAACGATCGGTACAGTATGG
  TCGGAGGAAACCTTGTTATCAACAACCCGGACAAACAGAAAGACGCTGGGGTGTACTACTGCCTGGCATC
  CAACAACTACGGAATGGTCAGGAGCACGGAGGCAACTCTGAGCTTCGGGTATCTCGACCCCTTTCCTCCC
  GAGGAGCGTCCGGAAGTGAAGGTGAAGGAAGGGAAGGGCATGGTGCTTCTCTGTGATCCTCCCTACCACT
  TCCCAGATGATCTTAGCTACCGCTGGCTTCTGAATGAATTTCCTGTATTTATCACCATGGATAAGCGAAG
  ATTTGTGTCTCAGACCAACGGCAATCTCTACATCGCAAATGTCGAGTCTTCTGACAGAGGCAACTACTCC
  TGCTTTGTGTCCAGTCCTTCTATTACAAAGAGTGTGTTCAGCAAGTTTATTCCCCTCATTCCGATTCCTG
  AACGAACAACAAAGCCATATCCTGCTGATATTGTGGTGCAGTTCAAGGACATATACACCATGATGGGCCA
  AAATGTGACTTTAGAGTGTTTCGCTCTAGGGAACCCTGTTCCTGATATCCGGTGGCGGAAGGTGCTAGAA
  CCAATGCCCAGCACTGCTGAGATAAGCACGTCAGGGGCTGTCCTCAAGATCTTCAACATCCAGCTAGAAG
  ATGAAGGCCTCTACGAGTGTGAGGCTGAGAACATCCGGGGAAAGGACAAGCACCAGGCAAGAATCTATGT
  TCAAGCGTTTCCTGAGTGGGTAGAGCATATCAATGACACTGAGGTGGACATAGGCAGTGACCTCTACTGG
  CCTTGCATAGCCACAGGGAAGCCCATTCCTACCATCAGGTGGCTGAAAAATGGATACTCGTATCACAAAG
  GGGAACTGAGACTGTATGATGTGACTTTTGAAAATGCCGGGATGTACCAGTGCATAGCAGAAAACGCGTA
  TGGGTCCATTTATGCCAACGCTGAGCTCAAGATCCTGGCTTTAGCTCCAACTTTTGAAATGAATCCTATG
  AAGAAAAAGATCTTGGCTGCGAAAGGTGGAAGGGTTATCATTGAATGTAAACCCAAGGCCGCACCAAAAC
  CCAAGTTTTCATGGAGCAAAGGGACGGAGTGGCTCGTTAACAGCAGCAGAATACTCATATGGGAAGATGG
  TAGCTTGGAAATCAATAATATTACAAGAAATGATGGTGGAATCTATACATGCTTTGCAGAAAATAACAGA
  GGAAAAGCTAATAGCACTGGGACTCTGGTGATCACAAATCCCACACGAATTATTTTGGCCCCAATCAATG
  CTGACATCACTGTTGGGGAAAATGCCACCATGCAGTGTGCTGCGTCTTTCGATCCTGCCTTGGACCTCAC
  GTTTGTGTGGTCCTTCAATGGCTACGTGATTGACTTTAACAAGGAAATTACGCATATCCATTACCAGAGG
  AATTTTATGCTCGACGCCAATGGGGAACTGCTCATCCGAAATGCGCAGCTGAAACACGCAGGCCGGTACA
  CGTGCACGGCGCAGACAATCGTGGACAATTCCTCGGCTTCCGCTGACCTTGTCGTTCGAGGCCCCCCAGG
  TCCCCCAGGAGGCCTGAGAATAGAAGACATCAGAGCTACCTCTGTGGCACTGACTTGGAGCCGTGGTTCC
  GACAATCACAGCCCTATCTCTAAATACACGATCCAGACCAAGACGATTCTCTCAGATGACTGGAAAGATG
  CGAAGACAGATCCCCCAATCATTGAAGGAAACATGGAGTCAGCAAAAGCAGTGGATTTGATCCCTTGGAT
  GGAGTATGAATTTCGAGTGGTGGCGACCAATACTTTAGGGACAGGAGAGCCCAGCATACCCTCAAACAGG
  ATCAAAACAGACGGTGCTGCACCAAATGTGGCTCCCTCAGATGTAGGCGGTGGGGGCGGAACCAACAGAG
  AGCTGACAATAACGTGGGCGCCTTTGTCGAGAGAGTACCATTACGGTAACAATTTTGGTTACATAGTGGC
  GTTTAAGCCATTTGATGGAGAAGAGTGGAAGAAAGTGACTGTGACTAACCCAGACACTGGCCGCTACGTC
  CACAAAGATGAGACCATGACCCCCTCCACCGCGTTTCAAGTCAAAGTGAAAGCCTTCAACAATAAAGGAG
  ATGGGCCCTACAGCCTCGTAGCTGTCATCAACTCTGCACAAGATGCTCCCAGTGAAGCCCCCACAGAGGT
  AGGGGTCAAAGTGTTATCATCCTCTGAGATATCTGTCCATTGGAAGCATGTGTTAGAGAAAATTGTGGAA
  AGCTATCAGATTCGGTACTGGGCTGGGCATGACAAAGAAGCAGCCGCACACAGAGTTCAGGTCACCAGCC
  AGGAGTACTCAGCCAGACTGGAGAACCTTCTTCCCGACACCCAGTACTTCATTGAAGTGGGAGCCTGCAA
  CAGTGCCGGATGCGGACCTTCCAGTGATGTGATCGAGACCTTCACCAGAAAAGCCCCTCCTAGCCAACCA
  CCAAGGATCATCAGTTCGGTGAGATCTGGCTCACGATATATAATCACATGGGATCACGTTGTGGCACTAT
  CCAATGAATCTACTGTGACAGGCTACAAGATACTCTATAGACCGGATGGCCAACATGACGGCAAGCTGTT
  CTCAACCCATAAACACTCCATAGAAGTCCCCATCCCCAGAGACGGAGAGTATGTTGTCGAGGTTCGAGCA
  CACAGCGACGGGGGAGACGGAGTCGTGTCTCAAGTCAAAATTTCAGGCGTGTCCACGCTGTCTTCGAGTC
  TCCTCAGCTTGCTCCTGCCCTCCCTTGGCTTTCTTGTCTACTCGGAATTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226639 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKMPLLVSHLLLISLTSCLGDFTWHRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARAS
  PFPVYKWRMNNGDVDLTNDRYSMVGGNLVINNPDKQKDAGVYYCLASNNYGMVRSTEATLSFGYLDPFPP
  EERPEVKVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVESSDRGNYS
  CFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDIYTMMGQNVTLECFALGNPVPDIRWRKVLE
  PMPSTAEISTSGAVLKIFNIQLEDEGLYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYW
  PCIATGKPIPTIRWLKNGYSYHKGELRLYDVTFENAGMYQCIAENAYGSIYANAELKILALAPTFEMNPM
  KKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNR
  GKANSTGTLVITNPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKEITHIHYQR
  NFMLDANGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGS
  DNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMESAKAVDLIPWMEYEFRVVATNTLGTGEPSIPSNR
  IKTDGAAPNVAPSDVGGGGGTNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYV
  HKDETMTPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWKHVLEKIVE
  SYQIRYWAGHDKEAAAHRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPSSDVIETFTRKAPPSQP
  PRIISSVRSGSRYIITWDHVVALSNESTVTGYKILYRPDGQHDGKLFSTHKHSIEVPIPRDGEYVVEVRA
  HSDGGDGVVSQVKISGVSTLSSSLLSLLLPSLGFLVYSEF
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159647
• ORF Size: 3060 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159647.1, NP_001153119.1
• RefSeq Size: 5817 bp
• RefSeq ORF: 3063 bp
• Locus ID: 12805
• UniProt ID: P12960
• Cytogenetics: 15 46.39 cM
• MW: 113.4 kDa
• Gene Summary: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.[UniProtKB/Swiss-Prot Function]