Dclk1 (NM_001195538) Mouse Tagged ORF Clone
CAT#: MR226414
- TrueORF®
Dclk1 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 1 (Dclk1), transcript variant 5
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001195538" in other vectors (4)
Interest in protein/lysate? Submit request here!
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Dclk1 |
Synonyms | 1700113D08Rik; 2810480F11Rik; AI836758; Clic; Click-I; CPG1; Cpg16; Dc; Dcamk; Dcamkl1; Dcl; Dclk; mKIAA0369 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226414 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTTCGGCAGAGATATGGAGTTGGAGCATTTTGATGAGCGGGACAAGGCGCAGAGGTACAGCAGGG GGTCCCGTGTGAATGGCCTGCCCAGCCCCACACACAGCGCCCACTGCAGCTTCTACCGCACCCGCACCCT GCAGACGCTCAGCTCCGAGAAGAAAGCCAAGAAGGTTCGATTCTACAGAAATGGTGACCGCTACTTCAAA GGAATTGTGTATGCCATCTCCCCAGACCGCTTCAGATCTTTCGAGGCCCTGCTGGCTGATTTGACCCGAA CTCTCTCGGATAATGTGAATTTGCCCCAGGGGGTGAGAACCATCTACACCATCGATGGACTCAAGAAGAT CTCCAGCCTGGACCAGCTGGTGGAAGGTGAAAGCTATGTCTGCGGCTCCATCGAGCCCTTTAAGAAGCTG GAGTACACCAAGAATGTGAACCCCAACTGGTCAGTGAACGTCAAGACCACCTCAGCCTCCCGCGCAGTGT CTTCTTTGGCCACTGCCAAGGGTGGGCCTTCGGAGGTTCGGGAGAATAAGGATTTCATTCGACCCAAGCT GGTCACCATCATCAGAAGTGGGGTGAAGCCACGGAAGGCTGTCAGAATCCTGCTGAACAAGAAGACGGCT CACTCCTTCGAGCAGGTTCTCACTGACATTACCGACGCTATCAAGCTGGACTCCGGTGTGGTGAAGCGCC TGTACACTCTGGATGGGAAGCAGGTGATGTGCCTTCAGGACTTTTTTGGTGACGATGACATTTTTATTGC ATGTGGACCAGAGAAGTTCCGTTACCAGGATGATTTCTTGCTAGATGAAAGTGAATGTCGAGTGGTGAAA TCAACTTCTTACACCAAAATAGCATCAGCGTCCCGCAGAGGCACAACCAAGAGCCCAGGACCTTCCCGGA GAAGCAAGTCCCCAGCCTCCACCAGCTCAGTTAATGGAACCCCTGGTAGTCAGCTCTCTACTCCACGCTC GGGCAAGTCACCAAGTCCATCACCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGC GGCTCCTCGACTTCACTTTCATCCACTAAAGTTTGCAGCTCAATGGATGAGAATGATGGCCCTGGGGAAG AAGAGTCTGAGGAAGGCTTCCAGATTCCTGCCACAATAACAGAGAGATACAAAGTCGGGAGAACAATAGG AGACGGAAATTTTGCTGTTGTCAAGGAATGTATAGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATC ATCAAGAAAAGCAAATGCCGAGGCAAAGAGCACATGATCCAGAACGAGGTCTCCATCCTACGGAGGGTGA AGCACCCCAACATTGTCCTCCTGATTGAAGAGATGGATGTGCCGACTGAACTGTATCTTGTAATGGAATT AGTGAAGGGTGGAGACCTTTTCGATGCCATCACCTCCACTAGCAAATACACAGAGAGAGATGCCAGCGGG ATGCTGTACAACCTGGCCAGCGCCATCAAATACCTGCACAGCCTGAACATCGTCCACCGTGACATCAAGC CAGAGAATCTGCTGGTGTATGAGCACCAGGATGGCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGC CACAATTGTCGACGGCCCCCTGTACACAGTCTGTGGCACCCCAACATATGTGGCTCCAGAAATCATTGCA GAGACTGGATATGGCCTCAAGGTGGACATCTGGGCAGCTGGCGTGATCACTTATATCCTGCTGTGTGGCT TCCCTCCGTTCCGTGGAAGTGGGGATGACCAGGAGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGA CTTTCCATCTCCGTATTGGGACAATGTGTCAGATTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTT AACGTGGACCAGAGATTTTCAGCCGTGCAGGTCCTTGAGCATCCCTGGGTTAATGATGATGGTCTCCCAG AAAATGAGCATCAGCTGTCAGTAGCTGGCAAAATCAAGAAGCATTTCAACACAGGCCCCAAGCCGAGCAG CACTGCAGCAGGAGTTTCTGTAATAGCAACCACCGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGA CGCAACCAGGATGTGAGGAGCCGGTACAAGGCGCAGCCAGCTCCACCGGAATTGAACTCGGAATCGGAGG ACTACTCCCCCAGCTCCTCTGAGACTGTTCGCTCCCCCAATTCGCCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226414 protein sequence
Red=Cloning site Green=Tags(s) MSFGRDMELEHFDERDKAQRYSRGSRVNGLPSPTHSAHCSFYRTRTLQTLSSEKKAKKVRFYRNGDRYFK GIVYAISPDRFRSFEALLADLTRTLSDNVNLPQGVRTIYTIDGLKKISSLDQLVEGESYVCGSIEPFKKL EYTKNVNPNWSVNVKTTSASRAVSSLATAKGGPSEVRENKDFIRPKLVTIIRSGVKPRKAVRILLNKKTA HSFEQVLTDITDAIKLDSGVVKRLYTLDGKQVMCLQDFFGDDDIFIACGPEKFRYQDDFLLDESECRVVK STSYTKIASASRRGTTKSPGPSRRSKSPASTSSVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRISQHG GSSTSLSSTKVCSSMDENDGPGEEESEEGFQIPATITERYKVGRTIGDGNFAVVKECIERSTAREYALKI IKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASG MLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIA ETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLV NVDQRFSAVQVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPSSTAAGVSVIATTALDKERQVFRRR RNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001195538 |
ORF Size | 2223 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001195538.1, NP_001182467.1 |
RefSeq Size | 7817 bp |
RefSeq ORF | 2223 bp |
Locus ID | 13175 |
Cytogenetics | 3 C |
MW | 82.2 kDa |
Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities. [provided by RefSeq, Sep 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221217 | Dclk1 (untagged) - Mouse doublecortin-like kinase 1 (Dclk1), transcript variant 5, (10ug) |
USD 746.00 |
|
MG226414 | Dclk1 (tGFP-tagged) - Mouse doublecortin-like kinase 1 (Dclk1) transcript variant 5, (10ug) |
USD 879.00 |
|
MR226414L3 | Lenti ORF clone of Dclk1 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 1 (Dclk1), transcript variant 5 |
USD 979.00 |
|
MR226414L4 | Lenti ORF clone of Dclk1 (mGFP-tagged) - Mouse doublecortin-like kinase 1 (Dclk1), transcript variant 5 |
USD 979.00 |
{0} Product Review(s)
Be the first one to submit a review