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Myh7 (NM_080728) Mouse Tagged ORF Clone
CAT#: MR226406
- TrueORF®
Myh7 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 7, cardiac muscle, beta (Myh7)
ORF Plasmid: tGFP
"NM_080728" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Myh7 |
| Synonyms | B-MHC; beta-MHC; Myhc-b; MyHC-I; Myhcb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226406 representing NM_080728
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATGCAGAGATGGCTGCATTTGGGGCTGCAGCCCCCTTCCTGCGGAAGTCTGAGAAGGAGAGGC TGGAGGCACAGACCAGGCCCTTTGACCTCAAGAAAGATGTTTTTGTGCCCGATGACAAAGAAGAGTTTGT CAAGGCCAAGATCGTGTCCCGAGAGGGTGGCAAAGTCACTGCTGAGACGGAGAATGGCAAGACGGTGACT GTGAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA CCTTCCTGCATGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCTTCCTGGATGATCTATACCTA CTCGGGCCTCTTCTGCGTCACCGTCAACCCCTACAAGTGGCTGCCTGTGTACAATGCGGAAGTGGTGGCT GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCTCCTCACATCTTCTCCATCTCTGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAATCAGTCCATCCTCATCACCGGAGAATCCGGAGCTGGGAAGACTGTCAA CACTAAGAGGGTCATCCAATATTTTGCTGTTATTGCCGCCATTGGGGACCGCAGCAAGAAGGACCAGACC CCAGGCAAGGGTACCCTGGAAGATCAAATCATCCAAGCCAACCCTGCTCTGGAGGCCTTTGGCAATGCCA AGACAGTTCGGAATGACAACTCCTCTCGATTTGGGAAATTCATCCGAATCCATTTTGGGGCAACAGGAAA GTTGGCATCTGCAGACATAGAGACCTACCTTCTGGAAAAATCCAGGGTTATTTTCCAGCTGAAAGCAGAA AGAGATTATCACATTTTCTACCAAATCCTGTCTAATAAAAAGCCTGAGCTTCTAGACATGCTGCTGATCA CCAACAACCCCTACGATTATGCGTTCATCTCCCAAGGAGAGACGACTGTGGCCTCCATTGATGACTCTGA AGAGCTCATGGCCACGGATAGCGCCTTTGACGTGCTGGGCTTCACTCCAGAAGAGAAGAACTCCATTTAC AAGCTGACAGGCGCCATCATGCACTTTGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCGG AACCAGACGGCACTGAAGAGGCTGACAAATCAGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCTTAA GGGGCTGTGCCATCCTAGAGTCAAAGTGGGCAACGAGTACGTCACCAAGGGGCAGAATGTCCAGCAGGTG TCATACGCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCATCA ATGCAACCCTGGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGAGAT CTTCGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTCAAC CACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGACCTTCATAGACTTCG GCATGGACTTGCAGGCCTGCATTGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTTGAGGAGGA GTGCATGTTCCCCAAGGCCACAGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAGTCC AACAACTTCCAGAAGCCTCGAAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCTTTGGTCCACTATGCTG GCACTGTGGACTACAATATCCTGGGCTGGCTACAGAAGAACAAGGACCCACTCAATGAGACGGTGGTGGG TTTGTACCAGAAGTCTTCCCTCAAGCTGCTCAGCAATCTATTTGCCAACTATGCTGGAGCTGATGCCCCG GCGGACAAAGGCAAAGGCAAGGCAAAGAAAGGCTCATCCTTTCAGACCGTGTCTGCTCTGCACAGAGAAA ATCTGAACAAACTTATGACAAACTTGCGCTCCACGCACCCTCACTTTGTACGCTGCATCATCCCCAATGA GACAAAGTCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAATGGCGTGCTGGAG GGTATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATTCTCTATGGGGACTTCCGGCAGAGGTATCGCA TCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAACTGCTGGG CTCCCTGGACATTGACCACAACCAATACAAGTTTGGTCACACCAAGGTGTTCTTCAAGGCGGGCCTGCTG GGGCTGCTGGAGGAGATGCGTGATGAGAGGCTGAGCCGCATCATCACCAGAATCCAGGCCCAGTCCCGAG GTGTGCTCTCCAGAATGGAGTTCAAGAAGCTGCTGGAGCGCAGAGACTCCCTGCTGATTATCCAGTGGAA CATTAGGGCCTTCATGGGGGTCAAGAATTGGCCGTGGATGAAGCTCTACTTCAAGATCAAGCCGCTGCTG AAGAGCGCGGAGACGGAGAAGGAGATGGCCACCATGAAGGAGGAGTTTGGGCGAGTCAAAGATGCACTAG AGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAATGACCT GCAGCTCCAAGTGCAGGCGGAACAAGACAACTTGGCGGATGCAGAGGAGCGCTGTGACCAGCTGATCAAG AACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGATGAATG CCGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAGCGGGATATCGATGACCT GGAGCTGACGCTGGCCAAGGTGGAGAAGGAAAAGCATGCAACAGAGAACAAGGTGAAAAACCTGACAGAG GAGATGGCTGGTTTGGATGAGATCATTGTCAAGCTGACAAAGGAGAAGAAAGCTCTGCAAGAGGCCCACC AGCAGGCTCTGGATGACCTGCAGGCTGAGGAAGACAAGGTCAATACTCTGACCAAGGCCAAGGTCAAGCT GGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAGGTGCGCATGGACCTAGAGCGA GCCAAGCGGAAGCTGGAGGGAGACCTGAAGCTGACGCAGGAGAGCATCATGGACCTGGAGAATGACAAGC AGCAGTTGGATGAGCGACTCAAAAAGAAGGACTTTGAGTTAAATGCACTCAATGCCAGGATTGAGGATGA GCAAGCCCTGGGCAGTCAGCTGCAGAAGAAGCTCAAGGAGCTTCAGGCACGCATCGAGGAGCTGGAGGAG GAGCTGGAGGCCGAGCGCACAGCCCGGGCCAAGGTGGAGAAGCTGCGCTCTGACCTGTCCCGGGAGCTGG AGGAGATCAGTGAAAGGCTGGAGGAGGCAGGCGGGGCCACATCCGTGCAGATAGAGATGAACAAGAAGCG CGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACGGCGGCG GCCCTGCGCAAGAAGCATGCCGACAGCGTGGCGGAGCTGGGCGAGCAGATCGACAACCTCCAGCGGGTGA AGCAGAAGCTGGAGAAAGAGAAAAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACATGGAGCA GATCATCAAGGCCAAGGCTAACCTGGAGAAGATGTGCCGGACCTTGGAAGACCAGATGAATGAGCACCGG AGCAAGGCCGAGGAGACGCAGCGTTCTGTCAATGACCTCACCAGCCAGCGGGCCAAGCTGCAGACAGAGA ATGGGGAGCTGTCCCGGCAGCTGGACGAGAAGGAGGCTCTGATCTCTCAGCTAACCCGAGGCAAGCTCAC ATATACACAGCAGCTGGAGGACCTCAAGAGGCAACTGGAGGAGGAGGTCAAGGCCAAGAACGCGCTGGCC CACGCACTGCAGTCAGCACGGCATGATTGTGACCTGCTGAGGGAACAGTATGAGGAGGAGACAGAGGCCA AGGCTGAGCTACAGCGAGTCCTGTCCAAGGCCAATTCAGAGGTGGCCCAGTGGAGGACCAAGTATGAGAC GGATGCCATACAGAGGACAGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTGCAGGATGCA GAGGAGGCAGTGGAGGCTGTCAATGCCAAGTGTTCCTCTCTGGAGAAGACCAAGCACAGGCTGCAGAATG AGATCGAGGACCTGATGGTGGACGTGGAGCGCTCCAATGCCGCCGCCGCAGCCCTGGACAAGAAGCAGAG GAACTTTGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAGTCTTCC CAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTCTGGAGC ACCTAGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAGCAGCTGGG CTCCACGGGGAAGAGCATCCATGAGCTGGAGAAGATCCGAAAGCAACTGGAGGCCGAGAAGCTGGAGCTG CAGTCGGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGCGCCCAGCTAG AGTTCAACCAGATCAAGGCAGAGATTGAAAGGAAGCTGGCAGAGAAGGATGAGGAGATGGAGCAGGCCAA GCGCAACCACCTGCGGATGGTGGACTCCCTGCAGACCTCCCTGGATGCGGAGACACGCAGCCGCAATGAG GCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGCCATGCCAACC GTATGGCTGCTGAGGCCCAGAAACAAGTGAAGAGCCTCCAGAGTCTGCTGAAGGACACTCAAATCCAGCT GGATGATGCTGTCCGTGCCAATGACGACCTGAAAGAGAACATCGCCATCGTGGAACGGCGCAACAACCTG CTGCAGGCGGAGCTGGAGGAGCTTCGGGCTGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGGCAGAGC AGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCGCAGAACACCAGCCTCATCAACCAGAA GAAGAAGATGGATGCAGACCTATCCCAGCTCCAGACAGAAGTAGAGGAGGCAGTGCAGGAGTGTAGGAAC GCAGAGGAGAAGGCCAAGAAGGCTATCACAGATGCCGCCATGATGGCTGAGGAGCTGAAGAAGGAGCAGG ACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACTTGCAGCACCGTCT GGACGAGGCAGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGGGTCCGG GAGCTGGAGAATGAGCTGGAGGCTGAGCAAAAGCGCAATGCAGAGTCAGTGAAGGGCATGAGGAAGAGTG AGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAGGAAGAACCTACTGCGGCTGCAGGACCT GGTGGACAAGCTGCAGCTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAGCAGGCCAAC ACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCGGAGGAGAGGGCGGACATCGCCG AGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGGGACATTGGTGCCAAGGGCCTGAATGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226406 representing NM_080728
Red=Cloning site Green=Tags(s) MADAEMAAFGAAAPFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVT VKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVA AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQT PGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAE RDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIY KLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQV SYAIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFN HHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKS NNFQKPRNVKGKQEAHFSLVHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAP ADKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLE GIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLL GLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLL KSAETEKEMATMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIK NKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTE EMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLER AKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEE ELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAA ALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHR SKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALA HALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDA EEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESS QKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLEL QSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNE ALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNL LQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRN AEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVR ELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQAN TNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKGLNEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080728 |
| ORF Size | 5805 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080728.3 |
| RefSeq Size | 6054 bp |
| RefSeq ORF | 5808 bp |
| Locus ID | 140781 |
| UniProt ID | Q91Z83 |
| Cytogenetics | 14 28.01 cM |
| MW | 222.9 kDa |
| Gene Summary | Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.[UniProtKB/Swiss-Prot Function] |
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