Hfe2 (NM_027126) Mouse Tagged ORF Clone
CAT#: MR226289
- TrueORF®
Hfe2 (Myc-DDK-tagged) - Mouse hemochromatosis type 2 (juvenile) (human homolog) (Hfe2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_027126" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hfe2 |
Synonyms | 2310035L15Rik; 5230400G09Rik; AI414844; AI789733; DL-M; hemojuvelin; HJV; Rgmc |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226289 representing NM_027126
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAGTCCCCTAGTCCCCGGTCCCCCCACGGCAGCCCTCCAACTCTAAGCACCCTCACTCTCCTGC TGCTCCTCTGTGGACAGGCTCACTCCCAGTGCAAGATCCTCCGCTGCAATGCCGAGTATGTCTCGTCCAC TCTGAGTCTTCGGGGAGGTGGCTCACCGGACACGCCGCGTGGAGGCGGCCGTGGTGGGCTGGCCTCAGGT GGCTTGTGTCGCGCCCTGCGCTCCTACGCTCTCTGCACGCGGCGCACGGCCCGCACCTGCCGCGGGGACC TTGCTTTCCACTCTGCGGTGCATGGCATAGAGGACCTGATGATCCAGCACAACTGCTCACGCCAGGGTCC CACGGCCCCGCCCCCGGCCCGGGGCCCCGCCCTGCCCGGGGCCGGGCCAGCGCCCCTGACCCCAGATCCC TGTGACTATGAGGCCCGGTTTTCCAGGCTGCACGGTCGAGCCCCGGGCTTCTTGCATTGCGCATCCTTTG GAGATCCCCATGTGCGCAGTTTCCACAACCAATTTCACACATGCCGTGTCCAAGGAGCTTGGCCCTTGCT AGATAACGACTTCCTCTTTGTCCAGGCCACCAGCTCCCCGGTTTCGTCGGGAGCCAACGCTACCACCATC CGGAAGATCACTATCATATTTAAAAACATGCAGGAATGCATTGACCAGAAAGTCTACCAGGCTGAGGTGG ACAATCTTCCTGCAGCCTTTGAAGATGGTTCTATCAATGGGGGCGACCGACCTGGGGGCTCGAGTTTGTC CATTCAAACTGCTAACCTTGGGAGTCACGTGGAGATTCGAGCTGCCTACATTGGAACAACTATCATCATT CGACAGACAGCTGGGCAGCTCTCCTTCTCCATCAGGGTAGCAGAGGATGTGGCGCGGGCCTTCTCCGCAG AGCAGGACCTACAGCTGTGTGTTGGGGGATGCCCTCCGAGCCAGCGACTCTCTCGCTCAGAGCGCAACCG CCGTGGGGCTATAGCCATAGATACTGCCAGAAGGCTGTGTAAGGAAGGGCTTCCGGTTGAAGATGCCTAC TTCCAATCCTGCGTCTTTGATGTTTCAGTCTCCGGTGACCCCAACTTTACTGTGGCAGCTCAGACAGCTC TGGACGATGCCCGAATCTTCTTGACGGATTTAGAGAACTTACATCTCTTTCCCTCAGATGCGGGGCCTCC CCTCTCTCCTGCCATCTGCCTAGTCCCGCTTCTTTCGGCCCTCTTTGTTCTGTGGCTTTGCTTCAGTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226289 representing NM_027126
Red=Cloning site Green=Tags(s) MGQSPSPRSPHGSPPTLSTLTLLLLLCGQAHSQCKILRCNAEYVSSTLSLRGGGSPDTPRGGGRGGLASG GLCRALRSYALCTRRTARTCRGDLAFHSAVHGIEDLMIQHNCSRQGPTAPPPARGPALPGAGPAPLTPDP CDYEARFSRLHGRAPGFLHCASFGDPHVRSFHNQFHTCRVQGAWPLLDNDFLFVQATSSPVSSGANATTI RKITIIFKNMQECIDQKVYQAEVDNLPAAFEDGSINGGDRPGGSSLSIQTANLGSHVEIRAAYIGTTIII RQTAGQLSFSIRVAEDVARAFSAEQDLQLCVGGCPPSQRLSRSERNRRGAIAIDTARRLCKEGLPVEDAY FQSCVFDVSVSGDPNFTVAAQTALDDARIFLTDLENLHLFPSDAGPPLSPAICLVPLLSALFVLWLCFSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_027126 |
ORF Size | 1260 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027126.4, NP_081402.3 |
RefSeq Size | 2021 bp |
RefSeq ORF | 1263 bp |
Locus ID | 69585 |
UniProt ID | Q7TQ32 |
Cytogenetics | 3 F2.1 |
MW | 45.3 kDa |
Gene Summary | Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210967 | Hfe2 (untagged) - Mouse hemochromatosis type 2 (juvenile) (human homolog) (Hfe2), (10ug) |
USD 457.00 |
|
MG226289 | Hfe2 (tGFP-tagged) - Mouse hemochromatosis type 2 (juvenile) (human homolog) (Hfe2), (10ug) |
USD 657.00 |
|
MR226289L3 | Lenti ORF clone of Hfe2 (Myc-DDK-tagged) - Mouse hemochromatosis type 2 (juvenile) (human homolog) (Hfe2) |
USD 757.00 |
|
MR226289L4 | Lenti ORF clone of Hfe2 (mGFP-tagged) - Mouse hemochromatosis type 2 (juvenile) (human homolog) (Hfe2) |
USD 757.00 |
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