Musk (NM_001037127) Mouse Tagged ORF Clone
CAT#: MR226026
- TrueORF®
Musk (Myc-DDK-tagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001037127" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Musk |
Synonyms | Mdk4; Mlk; Ns; Nsk1; Nsk2; Nsk3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226026 representing NM_001037127
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGAGCTTGTCAACATTCCACTGTTACAGATGCTCACCCTGGTTGCCTTCAGCGGGACTGAGAAAC TTCCAAAAGCCCCTGTCATCACCACGCCTCTTGAAACTGTAGATGCCTTGGTTGAAGAAGTAGCGACTTT CATGTGTGCCGTGGAATCCTACCCTCAGCCCGAGATTTCTTGGACCAGAAATAAAATTCTCATTAAGCTG TTTGACACCCGCTACAGCATCCGGGAGAATGGTCAGCTCCTCACCATTCTGAGCGTGGAAGACAGTGATG ATGGCATCTACTGCTGCATAGCCAACAATGGAGTGGGAGGAGCCGTGGAGAGTTGTGGTGCCCTGCAAGT GAAGATGAAACCTAAAATAACTCGTCCTCCCATTAATGTAAAAATAATAGAGGGATTGAAGGCAGTTCTG CCGTGCACTACGATGGGTAACCCCAAACCATCTGTGTCCTGGATCAAGGGGGACAATGCTCTCAGGGAAA ATTCCAGAATCGCAGTTCTTGAATCTGGGAGCTTAAGGATCCATAATGTGCAAAAGGAAGATGCAGGACA GTACCGCTGTGTGGCCAAAAACAGCCTGGGCACAGCTTACTCCAAACTGGTGAAGCTGGAAGTGGAGGAA GACAGAGAACCTGAGCAGGACGCTAAAGTTTTTGCAAGAATCCTGCGTGCTCCTGAATCCCACAATGTCA CCTTTGGTTCCTTTGTAACCCTACGCTGCACAGCAATAGGCATCCCTGTCCCCACCATCAGCTGGATTGA AAACGGAAATGCTGTTTCTTCAGGTTCCATTCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTC CAGCTCTTCATCACAAAGCCAGGACTCTACACATGCATAGCTACCAATAAGCACGGAGAAAAGTTCAGTA CCGCAAAGGCTGCAGCCACTGTCAGCATAGCAGTCCCTCCTCCGTGGTTTTCTATGGATACTTCTTTCCT ATGGACAGAATGGAGTAAGTCACAGAAAGACAGCCAAGGCTACTGTGCCCAGTACAGAGGGGAGGTGTGT GATGCAGTCCTGGCGAAAGATGCTCTTGTCTTCTTCAACACCTCCTACCGGGACCCCGAGGACGCCCAGG AGCTGCTGATCCACACTGCGTGGAATGAGCTGAAGGCTGTGAGTCCACTGTGCCGGCCAGCTGCTGAGGC TCTGCTGTGTAACCACCTCTTCCAAGAGTGCAGCCCTGGAGTGGTACCTACTCCCATGCCCATTTGCAGA GAGTACTGCCTGGCGGTAAAGGAGCTCTTCTGTGCAAAGGAATGGCAGGCAATGGAAGGAAAGGCCCACC GGGGCCTCTACAGATCTGGGATGCATCTCCTTCCGGTACCAGAGTGCAGCAAGCTTCCCAGCATGCACCG GGACCCCACAGCCTGCACAAGACTGCCATATTTAGATTATAAAAAAGAAAACATAACAACATTCCCGTCA ATAACGTCCTCCAGGCCGAGCGCGGACATTCCAAACCTGCCTGCCTCCACCTCTTCCTTTGCCGTCTCGC CTGCGTACTCCATGACCGTCATCATCTCCATCGTGTCCAGCTTTGCCCTGTTTGCTCTTCTCACCATCGC TACTCTCTATTGCTGCCGAAGGAGGAAAGAATGGAAAAATAAGAAAAGAGAGTCGACCGCGGTGACCCTC ACCACGTTGCCTTCCGAGCTCCTGCTGGATAGGCTCCATCCCAACCCCATGTACCAGAGGATGCCACTCC TTCTGAATCCTAAGTTGCTCAGCCTGGAGTATCCGAGGAATAACATTGAGTATGTCCGAGACATCGGAGA GGGGGCGTTTGGAAGAGTCTTCCAAGCAAGGGCCCCTGGCTTGCTGCCTTATGAACCTTTCACTATGGTG GCCGTGAAGATGCTTAAGGAAGAGGCCTCTGCAGACATGCAAGCGGACTTTCAGAGGGAGGCGGCCCTCA TGGCAGAGTTTGACAACCCCAACATTGTGAAACTCTTAGGTGTGTGTGCCGTTGGGAAGCCGATGTGTCT GCTCTTTGAATATATGGCCTATGGTGACCTCAATGAGTTCCTCCGAAGTATGTCCCCGCACACTGTTTGC AGCCTCAGCCACAGTGACCTGTCCACGAGGGCTCGGGTGTCTAGCCCTGGTCCTCCACCACTGTCCTGTG CAGAACAGCTCTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCCTACCTTTCAGAGCGCAAGTTTGTCCA CCGGGACTTAGCTACCAGGAACTGCCTGGTTGGGGAGACCATGGTGGTGAAAATTGCAGACTTTGGCCTC TCCAGGAACATCTATTCCGCAGACTACTACAAAGCTGATGGAAATGACGCCATCCCTATCCGCTGGATGC CGCCCGAGTCTATCTTCTACAACCGCTACACCACTGAGTCGGATGTATGGGCCTATGGTGTGGTCCTCTG GGAGATCTTCTCCTATGGGCTGCAGCCCTACTATGGAATGGCCCACGAGGAGGTCATTTACTATGTGAGA GATGGCAACATCCTCGCCTGCCCTGAGAACTGCCCCTTGGAACTGTACAACCTCATGCGCCTGTGTTGGA GCAAGCTGCCTGCTGATAGACCCAGCTTCTGCAGTATCCACAGGATCCTGCAGCGCATGTGCGAGAGAGC AGAGGGAACGGTGGGTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226026 representing NM_001037127
Red=Cloning site Green=Tags(s) MRELVNIPLLQMLTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKL FDTRYSIRENGQLLTILSVEDSDDGIYCCIANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVL PCTTMGNPKPSVSWIKGDNALRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEE DREPEQDAKVFARILRAPESHNVTFGSFVTLRCTAIGIPVPTISWIENGNAVSSGSIQESVKDRVIDSRL QLFITKPGLYTCIATNKHGEKFSTAKAAATVSIAVPPPWFSMDTSFLWTEWSKSQKDSQGYCAQYRGEVC DAVLAKDALVFFNTSYRDPEDAQELLIHTAWNELKAVSPLCRPAAEALLCNHLFQECSPGVVPTPMPICR EYCLAVKELFCAKEWQAMEGKAHRGLYRSGMHLLPVPECSKLPSMHRDPTACTRLPYLDYKKENITTFPS ITSSRPSADIPNLPASTSSFAVSPAYSMTVIISIVSSFALFALLTIATLYCCRRRKEWKNKKRESTAVTL TTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDIGEGAFGRVFQARAPGLLPYEPFTMV AVKMLKEEASADMQADFQREAALMAEFDNPNIVKLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVC SLSHSDLSTRARVSSPGPPPLSCAEQLCIARQVAAGMAYLSERKFVHRDLATRNCLVGETMVVKIADFGL SRNIYSADYYKADGNDAIPIRWMPPESIFYNRYTTESDVWAYGVVLWEIFSYGLQPYYGMAHEEVIYYVR DGNILACPENCPLELYNLMRLCWSKLPADRPSFCSIHRILQRMCERAEGTVGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001037127 |
ORF Size | 2679 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001037127.2, NP_001032204.2 |
RefSeq Size | 3422 bp |
RefSeq ORF | 2682 bp |
Locus ID | 18198 |
Cytogenetics | 4 31.87 cM |
MW | 100.1 kDa |
Gene Summary | This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222416 | Musk (untagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 1, (10ug) |
USD 1,228.00 |
|
MG226026 | Musk (tGFP-tagged) - Mouse muscle skeletal receptor tyrosine kinase (Musk) transcript variant 1, (10ug) |
USD 1,426.00 |
|
MR226026L3 | Lenti ORF clone of Musk (Myc-DDK-tagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 1 |
USD 1,526.00 |
|
MR226026L4 | Lenti ORF clone of Musk (mGFP-tagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 1 |
USD 1,526.00 |
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