B4galt5 (NM_019835) Mouse Tagged ORF Clone

CAT#: MR225689

B4galt5 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5)

Product Images

Circular map for MR225689

• 

  Coomassie blue staining of purified B4galt5 protein (Cat# TP525689). The protein was produced from HEK293T cells transfected with B4galt5 cDNA clone (Cat# MR225689) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B4galt5
• Synonyms: 9430078I07Rik; AW049941; AW539721
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225689 representing NM_019835
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCCCGCCGGGGGCTGCTGCGGCTGCCGCGCCGCTCGCTGCTCGCCGCGCTCTTCTTCTTCTCGC
  TGTCGTCCTCGCTGCTCTACTTCGTCTATGTGGCGCCCGGCATAGTGAACACCTACCTCTTCATGGTGCA
  GGCTCAGGGCATCCTACTGCGGGACAATGTGAGGACCATCGGTGCCCAGGTGTACGAGCAGGTGGTCCGC
  AGCGCCTATGCCAAGAGGAACAGCAGCCTGAATGACTCAGATTATCCTCTGGACTTGAACCACAGTGAAG
  CCTTTCCCCCAACCACGACCTTTCTTCCTGAAGATTTCACCTACTTTGCCAACCACCCTTGCCCAGAGAG
  GCTCCCTTCCATGAAGGGCCCGATAGATATAAACATGAGTGAGATCGCAATGGACGATATTCATGAGCTG
  TTCTCCAGAGACCCGGCCATTAAGCTCGGAGGGCACTGGAAGCCTGCAGACTGTGTGCCTCGGTGGAAGG
  TGGCTATCCTCATCCCCTTCCGGAACCGCCATGAGCACCTCCCGGTCCTCCTGAGACACCTGCTCCCCAT
  GCTTCAGCGCCAGCGCCTGCAGTTCGCCTTCTATGTGATCGAGCAAGTTGGCACCCAGCCCTTTAACCGA
  GCCATGCTTTTCAATGTTGGTTTTCAAGAAGCCATGAAGGACTTGGATTGGGATTGTCTGATCTTCCATG
  ACGTCGATCACATACCTGAGAGTGACCGAAACTACTATGGCTGCGGGCAGATGCCCAGGCACTTTGCAAC
  CAAGCTGGACAAGTACATGTACCTGCTGCCCTACACTGAGTTTTTTGGTGGCGTGAGCGGCCTGACTGTA
  GAACAGTTTCGGAAAATCAATGGCTTTCCTAATGCCTTCTGGGGCTGGGGCGGAGAAGATGACGACTTGT
  GGAACAGGGTACAAAATGCAGGCTATTCTGTGAGCCGGCCAGAAGGGGACACAGGAAAATACAAGTCCAT
  TCCTCACCACCATCGAGGAGAGGTCCAGTTTCTTGGCAGGTACGCTCTGCTGAGGAAGTCAAAGGAGCGG
  CAAGGACTGGATGGCCTCAACAACCTGAACTACTCTGCGAATGTCACGTACGACGCCTTGTATAAAAACA
  TAACTGTCAACTTGACACCCGAGCTGGCTCAGGTGACTGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225689 representing NM_019835
  Red=Cloning site Green=Tags(s)
  
  MRARRGLLRLPRRSLLAALFFFSLSSSLLYFVYVAPGIVNTYLFMVQAQGILLRDNVRTIGAQVYEQVVR
  SAYAKRNSSLNDSDYPLDLNHSEAFPPTTTFLPEDFTYFANHPCPERLPSMKGPIDINMSEIAMDDIHEL
  FSRDPAIKLGGHWKPADCVPRWKVAILIPFRNRHEHLPVLLRHLLPMLQRQRLQFAFYVIEQVGTQPFNR
  AMLFNVGFQEAMKDLDWDCLIFHDVDHIPESDRNYYGCGQMPRHFATKLDKYMYLLPYTEFFGGVSGLTV
  EQFRKINGFPNAFWGWGGEDDDLWNRVQNAGYSVSRPEGDTGKYKSIPHHHRGEVQFLGRYALLRKSKER
  QGLDGLNNLNYSANVTYDALYKNITVNLTPELAQVTEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019835
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019835.2, NP_062809.2
• RefSeq Size: 4205 bp
• RefSeq ORF: 1167 bp
• Locus ID: 56336
• UniProt ID: Q9JMK0
• Cytogenetics: 2 H3
• MW: 45.3 kDa
• Gene Summary: Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed:21057870, PubMed:23882130, PubMed:30114188). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (PubMed:30114188). Plays a role in the glycosylation of BMPR1A and regulation of its protein stability (PubMed:29415997). Essential for extraembryonic development during early embryogenesis (PubMed:20574042, PubMed:21057870).[UniProtKB/Swiss-Prot Function]