Vcan (NM_172955) Mouse Tagged ORF Clone

CAT#: MR225673

Vcan (Myc-DDK-tagged) - Mouse versican (Vcan), transcript variant 5

Product Images

Circular map for MR225673

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vcan
• Synonyms: 5430420N07Rik; 9430051N09; Cspg2; DPEAAE; hdf; NG2; PG-M; PG-M(V0); PG-M(V1)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225673 representing NM_172955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGATAAATATGAAAGGCATCTTATGGATGTGCTCAACCTTATTACTAACCCATGCACTACATCAAG
  CCAAAATGGAAACCAGCCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGGTCCTACCTTGTCATTTTTC
  AACCTTACCTACCTTACCACCCAATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAGATGGAA
  GTGGACAAAAATGGAAAAGATATAAAGGAGACGACTGTCTTGGTGGCCCAGAACGGAAATATCAAGATTG
  GTCAGGACTACAAGGGGCGAGTGTCCGTGCCTACACATCCCGATGATGTAGGTGATGCTTCCCTCACCAT
  GGTCAAACTCCGGGCTAGTGATGCAGGCGTCTACCGATGTGATGTCATGTATGGGATTGAAGACACTCAG
  GACACCATGTCACTGGCTGTGGATGGTGTTGTGTTTCACTACAGGGCAGCCACCAGCAGGTACACTCTGA
  ACTTTGCCGCTGCTCAACAGGCTTGTTTGGATATCGGGGCGGTCATAGCAAGCCCAGAGCAGCTGTTTGC
  CGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGATCAAACTGTCAGATATCCCATA
  CGGGCTCCCCGAGAGGGCTGTTACGGAGACATGATGGGGAAGGAAGGGGTTCGGACCTATGGATTCCGCT
  CTCCCCAGGAAACCTATGATGTGTATTGTTATGTGGATCATCTGGATGGCGATGTGTTCCACATCACTGC
  TCCCAGTAAGTTCACCTTCGAGGAGGCCGAAGCAGAGTGTACAAGCAGGGATGCGAGGCTGGCGACTGTT
  GGAGAACTTCAGGCAGCTTGGAGAAATGGCTTTGACCAATGCGATTACGGCTGGCTGTCGGATGCCAGCG
  TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGAGGTCTACTTGGGGTGAGAACCCTGTATCG
  TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACGTAAGTGT
  TTGATATCTCTTTTAAAAATACAGATTAAAAAGCTGCAGCAAATATATACCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225673 representing NM_172955
  Red=Cloning site Green=Tags(s)
  
  MLINMKGILWMCSTLLLTHALHQAKMETSPPVKGSLSGKVVLPCHFSTLPTLPPNYNTSEFLRIKWSKME
  VDKNGKDIKETTVLVAQNGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQ
  DTMSLAVDGVVFHYRAATSRYTLNFAAAQQACLDIGAVIASPEQLFAAYEDGFEQCDAGWLSDQTVRYPI
  RAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECTSRDARLATV
  GELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKRKC
  LISLLKIQIKKLQQIYTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172955
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172955.1, NP_766543.1
• RefSeq Size: 3745 bp
• RefSeq ORF: 1107 bp
• Locus ID: 13003
• Cytogenetics: 13 45.5 cM
• MW: 41.6 kDa