Ltbp1 (NM_019919) Mouse Tagged ORF Clone

CAT#: MR225521

Ltbp1 (Myc-DDK-tagged) - Mouse latent transforming growth factor beta binding protein 1 (Ltbp1), transcript variant 1

Product Images

Circular map for MR225521

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ltbp1
• Synonyms: 9430031G15Rik; 9830146M04; b2b1000Clo; Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225521 representing NM_019919
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGAGCCTGGCTCAGGTGGGGTCTCCTGCTCTGGGCAGGGCTGCTAGCGTGGTCTGCGCACGGCC
  GGGTGCGGAGGATCACGTACGTGGTGCGCCCGGGCCCGGGACTGCCGGCCGGAGCCTTGCCTCTGGCTGG
  GCCCCCGCGGACCTTCAACGTCGCACTGGACGCCAGGTACAGCCGGAGCTCAACAGCCGCCTCCAGCCGC
  GCCTTGGCTGGGCCCCCAGCCGAGCGGACCCGGCGCACCAGCCAGCCCGGCGGCGCGGCCCTGCCGGGGC
  TCCGATCTCCGCTGCCACCCGAGCCCGCGCGTCCGGGGGGGCCCAGCCGGCAGCTCCACTCTAAGGCGGG
  TGCTCAGACCGCGGTCACTCGCTTCGCCAAACATGGCAGGCAAGTCGTGCGCTCCCAAGTGCAGCAGGAC
  GCTCAGAGCGCCGGGGGCTCCAGACTGCAGGTTCAGCAGAAGCAGCAGCTGCAGGGGATCAACGTCTGTG
  GAGGGCAGTGCTGCCACGGGTGGAGTAAACCTCCTGGCTCCCAGAGGTGCACCAAACCCAGCTGTGTTCC
  GCCATGCCAGAACGGAGGGATGTGTCTACGGCCTCAGCTCTGTGTGTGCAAGCCAGGGTCAAAGGGCAAA
  GCCTGCGAGATCACAGCTGCCCAGGACACCATGCCGCCAGCCTTTGGAGGGCAGAATCCTGGGTCTTCGT
  GGGCCCCCCTTGAGCAAGCAGCAAAACATACGTCAACCAAGAAGGCAGACACCTTGCCCAGGGTCAGTCC
  AGTGGCCCAGATGACCTTGACCCTCAAGCCAAAGCCATCAATGGGACTCTCCCAGCAGATACATCCTCAA
  GTTGCACCCTTATCTTCCCAGAATGTGATGATTCGGCATGGCCAGACCCAGGAGTACCTGCTCAAACCCA
  AGTACTTCCCAGCCCCGAAGGTGGTCTCTGCGGAGCAATCCACCGAAGGCTCTTTCTCTTTAAGATATGG
  GCAGGAGCAAGGCACGGCACCCTTTCAGGTGAGTAACCACACTGGTCGCATCAAGGTGGTCTTTACTCCG
  AGCATCTGTAAAGTGACCTGCACCAAGGGCAACTGTCAGAACAGCTGCCAGAAGGGGAATACCACCACTC
  TCATTAGTGAGAACGGCCATGCAGCCGACACCCTGACAGCCACGAACTTCCGAGTGGTTATTTGCCATCT
  TCCGTGTATGAATGGTGGCCAGTGCAGTTCAAGGGACAAATGTCAGTGCCCTCCAAATTTCACAGGAAAG
  CTTTGCCAGATCCCTGTCCTTGGTGCCAGTATGCCCAAACTCTACCAGCACGCCCAGCAGCAGGGCAAGG
  CATTGGGGAGTCACGTCATCCATTCCACACATACCTTGCCTCTAACCATGACTAGCCAGCAAGGGGTCAA
  AGTGAAATTCCCCCCCAACATAGTCAATATCCATGTGAAACATCCTCCCGAGGCTTCTGTCCAGATACAC
  CAGGTTTCCAGGATTGACAGCCCAGGAGGCCAGAAGGTGAAGGAAGCCCAACCAGGCCAGTCCCAAGTCT
  CTTACCAAGGACTTCCGGTCCAGAAGACCCAGACAGTCCATTCTACGTACTCCCACCAGCAGCTTATTCC
  CCATGTGTATCCCGTGGCTGCTAAGACACAACTTGGCCGATGCTTCCAGGAAACCATTGGATCACAGTGT
  GGCAAAGCGCTCCCTGGCCTTTCCAAGCAAGAGGACTGCTGTGGGACAGTGGGGACGTCCTGGGGCTTTA
  ACAAATGCCAGAAGTGCCCCAAGAAACAATCCTATCATGGATATACTCAAATGATGGAATGCCTACAAGG
  ATATAAACGGGTTAACAACACCTTTTGTCAAGATATTAATGAATGTCAGCTACAAGGTGTATGCCCTAAT
  GGTGAGTGTTTGAATACCATGGGCAGCTACAGATGCTCCTGCAAAATGGGATTCGGGCCTGACCCTACCT
  TTTCAAGTTGTGTTCCGGATCCCCCTGTGATCTCAGAAGAGAAAGGGCCCTGTTACCGCCTTGTCAGTCC
  TGGAAGGCATTGTATGCACCCTCTCTCTGTTCATCTCACCAAGCAGATCTGCTGTTGTAGTGTGGGCAAG
  GCCTGGGGCCCACATTGTGAGAAATGTCCCCTCCCAGGCACAGCTGCTTTTAAGGAAATCTGTCCTGGTG
  GAATGGGTTATACGGTTTCTGGCGTTCATAGACGCAGGCCAATCCATCAGCATATAGGTAAAGAAGCTGT
  ATATGTCAAGCCAAAGAACACTCAACCTGTTGCTAAAAGTACTCATCCTCCACCTCTCCCAGCCAAGGAA
  GAGCCTGTGGAGGCCCTGACCTCCTCGTGGGAACACGGGCCGAGAGGGGCGGAGCCCGAAGTGGTAACAG
  CACCTCCTGAGAAGGAAATACCTTCACTGGATCAAGAGAAAACCAGACTTGAACCTGGGCAACCCCAGCT
  CTCCCCAGGGGTTTCAACTATTCATCTGCACCCACAGTTTCCAGTAGTGGTTGAAAAGACATCCCCTCCT
  GTGCCTGTGGAAGTAGCTCCTGAAGCTTCCACATCCAGTGCCAGCCAAGTGATTGCTCCTACGCAAGTAA
  CAGAAATCAACGAATGTACTGTGAACCCGGACATTTGCGGAGCAGGACACTGCATCAACCTGCCGGTGCG
  GTATACCTGCATATGCTACGAGGGCTACAAGTTCAGTGAACAGCTGAGGAAATGTGTCGACATTGATGAA
  TGTGCTCAGGTCCGGCACCTCTGCTCCCAGGGTCGCTGTGAGAACACAGAGGGAAGTTTCCTGTGTGTCT
  GCCCGGCAGGGTTTATGGCCAGTGAGGAGGGTACTAACTGTATAGATGTGGATGAGTGCCTGAGGCCTGA
  TATGTGTAGGGATGGCCGCTGCATCAACACCGCTGGGGCCTTCCGGTGCGAGTACTGTGACAGTGGGTAC
  CGGATGTCACGGAGAGGCTACTGTGAGGATATTGATGAGTGTCTGAAGCCAAGCACTTGTCCCGAGGAAC
  AGTGTGTGAACACCCCGGGCTCTTACCAGTGTGTGCCCTGCACAGAGGGGTTCCGCGGCTGGAATGGACA
  ATGCCTTGATGTGGACGAGTGCCTGCAGCCAAAGGTCTGTACGAATGGTTCCTGCACCAACCTGGAAGGG
  TCCTACATGTGTTCCTGCCACAGGGGCTACAGCCCCACACCAGACCATAGACACTGTCAAGATATTGATG
  AATGCCAACAAGGGAATCTCTGCATGAATGGGCAGTGCAGAAATACCGATGGCTCCTTCCGGTGTACCTG
  TGGACAGGGCTATCAGCTGTCAGCAGCTAAAGACCAATGTGAAGATATTGATGAATGCGAGCACCATCAC
  CTCTGCTCTCACGGGCAGTGCAGGAACACAGAGGGCTCCTTCCAGTGTGTGTGCAACCAGGGTTACAGAG
  CATCTGTACTTGGAGACCACTGTGAGGATATCAATGAATGCTTGGAGGACAGCAGTGTCTGCCAGGGAGG
  TGACTGCATCAATACTGCGGGGTCCTATGATTGCACATGCCCGGATGGATTCCAGCTGAACGACAATAAG
  GGCTGTCAAGACATTAATGAATGTGCACAGCCAGGACTCTGTGGATCTCATGGGGAGTGTCTAAACACAC
  AAGGCTCCTTCCACTGTGTCTGTGAACAGGGGTTCTCCATCTCTGCCGATGGCCGTACATGCGAAGATAT
  TGATGAGTGTGTTAACAACACTGTGTGTGACAGTCACGGCTTCTGTGACAACACAGCCGGCTCTTTCCGC
  TGCCTCTGTTATCAGGGCTTTCAAGCCCCACAGGATGGGCAAGGGTGTGTGGATGTGAACGAGTGTGAAC
  TGCTCAGTGGTGTATGTGGGGAAGCCTTCTGTGAAAACGTGGAAGGATCCTTCCTGTGTGTGTGTGCCGA
  TGAGAACCAGGAGTACAGCCCCATGACTGGGCAGTGTCGCTCTCGGGTCACGGAAGATTCAGGTGTGGAT
  CGTCAGCCTAGAGAAGAGAAGAAGGAGTGCTATTATAACCTCAATGATGCTAGTCTCTGTGATAATGTCC
  TGGCCCCCAACGTCACCAAACAAGAGTGCTGCTGTACGTCAGGTGCCGGCTGGGGAGACAACTGTGAGAT
  CTTCCCTTGCCCCGTCCAGGGAACTGCTGAGTTCACGGAAATGTGCCCTAGAGGGAAAGGTTTGGTCCCG
  GCTGGAGAATCCTCTTACGACACTGGTGGCGAGAACTACAAAGATGCTGATGAATGCTTGCTGTTTGGAG
  AGGAAATCTGTAAAAATGGTTACTGTTTGAACACTCAACCTGGGTATGAATGCTACTGTAAGCAAGGGAC
  ATACTATGATCCTGTCAAATTACAGTGCTTTGATATGGATGAATGCCAAGACCCTAACAGTTGCATTGAC
  GGCCAGTGTGTGAACACAGAGGGCTCCTACAACTGCTTTTGCACCCACCCAATGGTCCTGGATGCCTCTG
  AGAAGAGATGTGTGCAACCAACTGAATCAAATGAACAAATAGAAGAGACCGATGTCTATCAAGATCTGTG
  CTGGGAGCATCTGAGTGAGGAGTACGTGTGTAGCCGTCCTCTCGTAGGCAAGCAGACGACGTACACAGAA
  TGCTGCTGTTTGTACGGGGAGGCTTGGGGCATGCAGTGTGCTCTGTGCCCCATGAAGGACTCAGATGACT
  ATGCCCAGCTGTGCAATATCCCTGTGACTGGGCGTCGGCGGCCATATGGACGGGATGCATTGGTGGACTT
  CAGTGAACAGTATGGCCCAGAAACAGACCCTTACTTCATCCAGGATCGCTTTCTAAATAGCTTTGAGGAG
  CTACAGGCTGAGGAGTGTGGCATCCTCAATGGTTGTGAAAACGGCCGCTGTGTGAGAGTCCAGGAAGGAT
  ACACTTGCGATTGCTTTGATGGATATCACCTGGATATGGCCAAGATGACCTGTGTCGATGTAAATGAATG
  CAGCGAGCTGAATAATCGGATGTCTCTCTGCAAGAACGCCAAGTGCATTAACACAGAAGGCTCCTACAAA
  TGCTTGTGTCTGCCAGGCTACATACCATCCGACAAGCCCAACTACTGTACACCCCTGAACAGCGCTTTGA
  ATTTAGACAAAGAAAGTGACCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225521 representing NM_019919
  Red=Cloning site Green=Tags(s)
  
  MAGAWLRWGLLLWAGLLAWSAHGRVRRITYVVRPGPGLPAGALPLAGPPRTFNVALDARYSRSSTAASSR
  ALAGPPAERTRRTSQPGGAALPGLRSPLPPEPARPGGPSRQLHSKAGAQTAVTRFAKHGRQVVRSQVQQD
  AQSAGGSRLQVQQKQQLQGINVCGGQCCHGWSKPPGSQRCTKPSCVPPCQNGGMCLRPQLCVCKPGSKGK
  ACEITAAQDTMPPAFGGQNPGSSWAPLEQAAKHTSTKKADTLPRVSPVAQMTLTLKPKPSMGLSQQIHPQ
  VAPLSSQNVMIRHGQTQEYLLKPKYFPAPKVVSAEQSTEGSFSLRYGQEQGTAPFQVSNHTGRIKVVFTP
  SICKVTCTKGNCQNSCQKGNTTTLISENGHAADTLTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGK
  LCQIPVLGASMPKLYQHAQQQGKALGSHVIHSTHTLPLTMTSQQGVKVKFPPNIVNIHVKHPPEASVQIH
  QVSRIDSPGGQKVKEAQPGQSQVSYQGLPVQKTQTVHSTYSHQQLIPHVYPVAAKTQLGRCFQETIGSQC
  GKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKQSYHGYTQMMECLQGYKRVNNTFCQDINECQLQGVCPN
  GECLNTMGSYRCSCKMGFGPDPTFSSCVPDPPVISEEKGPCYRLVSPGRHCMHPLSVHLTKQICCCSVGK
  AWGPHCEKCPLPGTAAFKEICPGGMGYTVSGVHRRRPIHQHIGKEAVYVKPKNTQPVAKSTHPPPLPAKE
  EPVEALTSSWEHGPRGAEPEVVTAPPEKEIPSLDQEKTRLEPGQPQLSPGVSTIHLHPQFPVVVEKTSPP
  VPVEVAPEASTSSASQVIAPTQVTEINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQLRKCVDIDE
  CAQVRHLCSQGRCENTEGSFLCVCPAGFMASEEGTNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGY
  RMSRRGYCEDIDECLKPSTCPEEQCVNTPGSYQCVPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEG
  SYMCSCHRGYSPTPDHRHCQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHHH
  LCSHGQCRNTEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGSYDCTCPDGFQLNDNK
  GCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFR
  CLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRVTEDSGVD
  RQPREEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFTEMCPRGKGLVP
  AGESSYDTGGENYKDADECLLFGEEICKNGYCLNTQPGYECYCKQGTYYDPVKLQCFDMDECQDPNSCID
  GQCVNTEGSYNCFCTHPMVLDASEKRCVQPTESNEQIEETDVYQDLCWEHLSEEYVCSRPLVGKQTTYTE
  CCCLYGEAWGMQCALCPMKDSDDYAQLCNIPVTGRRRPYGRDALVDFSEQYGPETDPYFIQDRFLNSFEE
  LQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDMAKMTCVDVNECSELNNRMSLCKNAKCINTEGSYK
  CLCLPGYIPSDKPNYCTPLNSALNLDKESDLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019919
• ORF Size: 5136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019919.4, NP_064303.2
• RefSeq Size: 7455 bp
• RefSeq ORF: 5139 bp
• Locus ID: 268977
• UniProt ID: Q8CG19
• Cytogenetics: 17 E2
• MW: 186.7 kDa
• Gene Summary: Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta.[UniProtKB/Swiss-Prot Function]