Thrb (NM_001113417) Mouse Tagged ORF Clone

CAT#: MR225396

Thrb (Myc-DDK-tagged) - Mouse thyroid hormone receptor beta (Thrb), transcript variant 1

Product Images

Circular map for MR225396

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thrb
• Synonyms: c-erbAbeta; Nr1a2; T3Rbeta; T3R[b]; Thrb1; Thrb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225396 representing NM_001113417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCCTAACAGTATGACAGAAAATGGCCTTCCAGCCTGGGACAAACAGAAGCCCCGTCCAGACCGAG
  GCCAGGACTGGAAGCTGGTAGGAATGTCTGAAGCCTGCCTGCACAGGAAGAGCCACGTGGAGAGGCGTGG
  TGCACTGAAGAATGAGCAGACTTCCCCACACCTTATCCAGGCCACTTGGACAAGCTCTATATTCCACCTG
  GATCCTGACGATGTGAACGATCAGAGCATCTCAAGTGCCCAGACTTTCCAGACTGAAGAGAAGAAATGTA
  AAGGGTATATCCCCAGCTATTTAGACAAAGATGAGCTCTGTGTAGTGTGTGGGGACAAAGCCACAGGGTA
  CCACTATCGCTGCATCACCTGTGAAGGCTGCAAGGGCTTCTTTAGAAGAACCATTCAGAAAAGTCTCCAT
  CCATCTTATTCCTGTAAATATGAAGGAAAGTGCATCATAGACAAAGTCACCCGCAACCAGTGCCAGGAAT
  GTCGCTTTAAGAAATGCATCTATGTTGGCATGGCAACAGACTTGGTGCTGGATGACAGCAAGAGGCTAGC
  CAAGCGGAAGCTTATAGAGGAGAACCGTGAGAAGAGGCGGCGGGAAGAGCTACAGAAATCAATTGGGCAC
  AAGCCAGAACCCACGGATGAGGAGTGGGAGCTCATCAAGACAGTCACTGAGGCCCACGTGGCCACCAATG
  CCCAGGGCAGCCACTGGAAGCAGAAGCGGAAATTCCTGCCAGAAGACATTGGACAAGCACCCATCGTGAA
  TGCCCCAGAAGGTGGCAAGGTTGACCTGGAAGCCTTCAGTCATTTTACAAAAATCATCACACCAGCAATT
  ACCAGAGTGGTGGATTTTGCCAAAAAGTTGCCCATGTTTTGTGAGCTGCCATGTGAAGACCAGATCATCC
  TCCTCAAAGGCTGCTGCATGGAGATCATGTCCCTTCGAGCTGCTGTGCGCTATGACCCAGACAGCGAGAC
  TCTAACTTTGAATGGGGAAATGGCAGTGACACGAGGCCAGCTGAAAAATGGGGGCCTTGGGGTGGTTTCA
  GATGCCATCTTTGACCTGGGCATGTCTCTGTCATCTTTCAACCTGGATGACACTGAAGTCGCCCTGCTTC
  AGGCCGTCCTGCTAATGTCTTCAGATCGCCCAGGGCTAGCCTGTGTTGAGAGAATAGAGAAATACCAAGA
  CAGTTTCCTGTTGGCCTTTGAACACTATATCAATTACCGGAAGCACCATGTGACACACTTTTGGCCCAAA
  CTCCTGATGAAGGTGACAGACCTGCGGATGATTGGAGCTTGCCATGCCAGCCGCTTCCTCCACATGAAGG
  TGGAGTGCCCCACTGAGCTCTTCCCGCCTCTGTTCTTAGAAGTATTTGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225396 representing NM_001113417
  Red=Cloning site Green=Tags(s)
  
  MTPNSMTENGLPAWDKQKPRPDRGQDWKLVGMSEACLHRKSHVERRGALKNEQTSPHLIQATWTSSIFHL
  DPDDVNDQSISSAQTFQTEEKKCKGYIPSYLDKDELCVVCGDKATGYHYRCITCEGCKGFFRRTIQKSLH
  PSYSCKYEGKCIIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEENREKRRREELQKSIGH
  KPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAI
  TRVVDFAKKLPMFCELPCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLNGEMAVTRGQLKNGGLGVVS
  DAIFDLGMSLSSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPK
  LLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113417
• ORF Size: 1383 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113417.1, NP_001106888.1
• RefSeq Size: 5997 bp
• RefSeq ORF: 1386 bp
• Locus ID: 21834
• UniProt ID: P37242
• Cytogenetics: 14 A2
• MW: 53.1 kDa
• Gene Summary: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.[UniProtKB/Swiss-Prot Function]