Tnr (NM_022312) Mouse Tagged ORF Clone

CAT#: MR225166

Tnr (Myc-DDK-tagged) - Mouse tenascin R (Tnr)

Product Images

Circular map for MR225166

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnr
• Synonyms: J1-tenascin; janusin; restrictin; TN-R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225166 representing NM_022312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATCGATGGGGAAACAGTAGTCTTGAAGAACATGCTCATTGGTGTAAACCTGATCCTGTTGGGAT
  CCATGCTCAAGCCTTCTGAGTGTCGGCTGGAGGTGACTACAGAAAGGGCTCAGAGACAAACTGTGGAGGA
  GGAAGGAGGGGCTTCCAGCTACAATACTTCCAGCAAGGAACAGCCTATGGTCTTCAACCATGTGTATAAC
  ATCAACGTGCCACTTGAAAGCCTCTGCTCCTCGGGGCTGGAGGCCTCAGCTGAGCAGGACATGAGTGCTG
  AAGATGATACTCTGGCCGAATACATAGGCCAAACCTCAGACCATGAAAGCCAGGTTACCTTCACCCACAA
  GATCAACCTCCCCAAAAAAGCCTGCCCATGTGCAAGCTCTTCCCAGGTACTGCAGGAACTGCTGAGCCGG
  ATCGAGATGCTGGAGAGGGAGGTGTCCTTGCTGCGAGACCAGTGCAATACCAACTGCTGTCAGGAAAGCG
  CTGCCACAGGACAACTGGACTATGTCCCTCACTGCAGCGGCCATGGCAACTTTAGCTTCGAGTCCTGTGG
  CTGCATCTGCAATGAAGGCTGGTTTGGCAAGAACTGCTCAGAGCCCTACTGCCCACTGGGCTGCTCCAGT
  CGGGGTGTGTGTGTCGATGGCCAGTGCATTTGTGACAGTGAATACAGCGGAGATGACTGTTCAGAGCTCC
  GGTGCCCAACAGACTGCAGTTCCCGAGGGCTCTGTGTGGATGGGGAATGTGTCTGTGAAGAGCCCTACAC
  AGGCGAGGACTGCAGGGAGCTGCGCTGCCCTGGGGACTGTTCAGGGAAGGGGCAATGTGCCAATGGTACC
  TGCCTGTGCCAAGAGGGCTATGCTGGTGAGGACTGCAGCCAGCGACGGTGTCTGAATGCCTGCAGTGGGC
  GAGGTCACTGCCAGGAGGGGCTCTGCATCTGTGAGGAAGGCTACCAGGGCCCTGACTGCTCAGCAGTTGC
  CCCTCCAGAGGACTTGCGAGTGGCTGGTATCAGCGACAGGTCCATTGAGCTGGAATGGGACGGGCCGATG
  GCAGTGACGGAATATGTGATCTCTTACCAGCCGACGGCCCTGGGGGGCCTTCAGCTCCAGCAGCGGGTGC
  CTGGAGATTGGAGTGGTGTCACCATCATGGAGCTGGAGCCAGGTCTCACCTACAACATCAGCGTCTACGC
  TGTCATTAGCAACATCCTCAGCCTTCCCATCACTGCCAAGGTGGCCACTCATCTCTCCACTCCTCAAGGG
  CTACAGTTCAAGACGATCACAGAGACCACCGTGGAGGTGCAGTGGGAGCCCTTCTCTTTCTCCTTCGACG
  GGTGGGAGATCAGCTTCATTCCAAAGAACAATGAAGGAGGGGTGATTGCTCAGCTCCCCAGCGATGTGAC
  GTCCTTTAACCAGACAGGACTGAAACCTGGGGAGGAGTACATTGTGAATGTTGTGGCTCTAAAGGAACAA
  GCCCGGAGCCCTCCTACCTCTGCCAGCGTCTCCACTGTCATTGACGGGCCCACACAGATCCTGGTTCGAG
  ATGTCTCTGATACTGTGGCTTTTGTGGAATGGACCCCACCTCGAGCCAAAGTTGATTTCATTCTTTTAAA
  ATATGGCTTGGTGGGTGGCGAAGGCGGGAAGACGACCTTCCGGCTGCAGCCTCCCTTAAGCCAGTACTCC
  GTGCAAGCCCTTAGACCTGGCTCGCGCTACGAGGTGTCCATCAGCGCAGTCCGGGGGACCAATGAGAGTG
  AAGCCTCAAGCACCCAATTTACAACAGAGATTGATGCTCCCAAGAATTTGCGAGTGGGTTCCCGCACAGC
  AACTAGCCTTGACCTGGAATGGGATAACAGCGAGGCCGAAGCTCAGGAGTACAAGGTTGTGTACAGCACC
  TTAGCTGGTGAACAGTACCACGAAGTGCTGGTACCCAAGGGCATTGGCCCAACTACCAAGACTACCCTCA
  CAGATCTGGTTCCAGGCACAGAGTATGGAGTTGGAATATCTGCAGTAATGAACTCAAAACAAAGCATTCC
  TGCCACTATGAATGCCAGGACTGAACTTGACAGTCCCCGAGACCTCATGGTAACAGCCTCCTCAGAGACC
  TCTATCTCTCTCATCTGGACCAAGGCCAGTGGTCCCATTGATCACTACAGAATTACTTTTACCCCATCTT
  CTGGGATCTCCTCCGAAGTCACTGTGCCCAGGGATAGGACTTCATACACACTAACAGATCTAGAGCCTGG
  AGCAGAATACATTATCTCCATCACTGCTGAGAGGGGTCGGCAGCAGAGCCTGGAGTCTACTGTGGATGCC
  TTCACAGGCTTCCGCCCTATCTCCCATTTGCACTTTTCTCATGTGACCTCCTCCAGTGTCAATATCACCT
  GGAGTGACCCATCTCCCCCAGCAGACAGACTCATTCTGAACTACAGCCCCAGGGACAAAGAGGAAGACAT
  GTTGGAGGTCCTCTTGGATGCCACCAAGAGGCACGCTGTCTTGATGGGTCTACAGCCAGCCACTGAATAT
  ATAGTGAACCTTGTAGCTGTCCATGGGACGGTAACCTCTGAACCCATAGTGGGTTCTATCACTACAGGAA
  TTGATCCTCCCAAAAACATCACAATTAGCAACGTGACTAAGGACTCCCTGACGGTGTCCTGGAGCTCTCC
  TGTTGCGCCTTTTGATTACTACCGAGTATCGTACCGACCCACCCAAGTGGGACGGCTAGACAGCTCCGTA
  GTGCCCAACACCGTGACAGAGTTCGCCATCACCAGGCTGTATCCAGCTACTGAATATGAAATAAGCCTCA
  ACAGTGTACGGGGCAGGGAGGAGAGTGAACGCATCTGCACCCTGGTGCACACAGCCATGGATAGCCCCAT
  GGATTTGATCGCTACCAACATCACACCTACAGAAGCCTTGCTCCAGTGGAAAGCACCCATGGGTGAAGTG
  GAAAATTATGTCATCGTCCTCACACACTTTGCAATTGCTGGAGAGACCATCCTGGTTGACGGGGTCAGCG
  AAGAATTCCAGCTTGTAGACTTGCTTCCTAGCACCCACTACACTGTCACTATGTATGCCACCAGTGGGCC
  TCTCATGAGTGGCACCATTGCCACCAACTTCTCCACCCTCCTGGACCCTCCTGACAACCTGACAGCCAGT
  GAAGTCACCAGGCAAAGCGCACTGATCTCCTGGCAGCCGCCCAGAGCTGCGATTGAAAACTATGTCTTGA
  CATACAAGTCCACCGATGGAAGCCGCAAAGAGCTGATAGTGGATGCTGAGGACACCTGGATCCGACTAGA
  GGGCCTGTCAGAGAACACAGACTACACAGTGCTCCTGCAGGCAGCCCAGGAGGCCACAAGGAGCAGTCTC
  ACCTCTACTGTCTTTACCACAGGGGGCCGGGTGTTCTCTCATCCTCAAGACTGTGCCCAGCATTTGATGA
  ATGGAGACACTCTGAGTGGCGTTTACACCATCTTCCTCAATGGGGAGCTAAGCCACAAGCTGCAAGTGTA
  CTGTGACATGACCACAGATGGGGGCGGCTGGATTGTATTCCAGAGGCGGCAAAATGGACAAACTGATTTT
  TTCCGGAAATGGGCAGATTATCGTGTTGGCTTTGGGAATCTGGAGGATGAGTTTTGGCTAGGGCTAGACA
  ACATCCACAGGATAACAGCCCAGGGCCGCTATGAGCTGCGTGTGGATATGCGGGATGGACAGGAAGCAGT
  CTTTGCCTACTATGACAAGTTCGCTGTGGAGGACAGCAGAAGCCTGTACAAGATCCGCATAGGAAGTTAC
  AATGGCACTGCAGGAGACTCCCTCAGCTATCACCAGGGACGTCCTTTCTCCACTGAGGACAGAGACAATG
  ATGTTGCAGTCACCAACTGTGCCATGTCCTACAAGGGTGCTTGGTGGTATAAGAACTGCCACCGGACCAA
  CCTCAACGGGAAGTACGGGGAGTCCAGGCACAGTCAGGGGATCAACTGGTACCATTGGAAAGGCCATGAA
  TTCTCCATCCCCTTTGTAGAAATGAAGATGAGGCCCTACATCCATCGTCTCACAGCCGGGAGGAAACGGC
  GAGCCTTGAAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225166 representing NM_022312
  Red=Cloning site Green=Tags(s)
  
  MGIDGETVVLKNMLIGVNLILLGSMLKPSECRLEVTTERAQRQTVEEEGGASSYNTSSKEQPMVFNHVYN
  INVPLESLCSSGLEASAEQDMSAEDDTLAEYIGQTSDHESQVTFTHKINLPKKACPCASSSQVLQELLSR
  IEMLEREVSLLRDQCNTNCCQESAATGQLDYVPHCSGHGNFSFESCGCICNEGWFGKNCSEPYCPLGCSS
  RGVCVDGQCICDSEYSGDDCSELRCPTDCSSRGLCVDGECVCEEPYTGEDCRELRCPGDCSGKGQCANGT
  CLCQEGYAGEDCSQRRCLNACSGRGHCQEGLCICEEGYQGPDCSAVAPPEDLRVAGISDRSIELEWDGPM
  AVTEYVISYQPTALGGLQLQQRVPGDWSGVTIMELEPGLTYNISVYAVISNILSLPITAKVATHLSTPQG
  LQFKTITETTVEVQWEPFSFSFDGWEISFIPKNNEGGVIAQLPSDVTSFNQTGLKPGEEYIVNVVALKEQ
  ARSPPTSASVSTVIDGPTQILVRDVSDTVAFVEWTPPRAKVDFILLKYGLVGGEGGKTTFRLQPPLSQYS
  VQALRPGSRYEVSISAVRGTNESEASSTQFTTEIDAPKNLRVGSRTATSLDLEWDNSEAEAQEYKVVYST
  LAGEQYHEVLVPKGIGPTTKTTLTDLVPGTEYGVGISAVMNSKQSIPATMNARTELDSPRDLMVTASSET
  SISLIWTKASGPIDHYRITFTPSSGISSEVTVPRDRTSYTLTDLEPGAEYIISITAERGRQQSLESTVDA
  FTGFRPISHLHFSHVTSSSVNITWSDPSPPADRLILNYSPRDKEEDMLEVLLDATKRHAVLMGLQPATEY
  IVNLVAVHGTVTSEPIVGSITTGIDPPKNITISNVTKDSLTVSWSSPVAPFDYYRVSYRPTQVGRLDSSV
  VPNTVTEFAITRLYPATEYEISLNSVRGREESERICTLVHTAMDSPMDLIATNITPTEALLQWKAPMGEV
  ENYVIVLTHFAIAGETILVDGVSEEFQLVDLLPSTHYTVTMYATSGPLMSGTIATNFSTLLDPPDNLTAS
  EVTRQSALISWQPPRAAIENYVLTYKSTDGSRKELIVDAEDTWIRLEGLSENTDYTVLLQAAQEATRSSL
  TSTVFTTGGRVFSHPQDCAQHLMNGDTLSGVYTIFLNGELSHKLQVYCDMTTDGGGWIVFQRRQNGQTDF
  FRKWADYRVGFGNLEDEFWLGLDNIHRITAQGRYELRVDMRDGQEAVFAYYDKFAVEDSRSLYKIRIGSY
  NGTAGDSLSYHQGRPFSTEDRDNDVAVTNCAMSYKGAWWYKNCHRTNLNGKYGESRHSQGINWYHWKGHE
  FSIPFVEMKMRPYIHRLTAGRKRRALKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022312
• ORF Size: 4074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022312.3, NP_071707.2
• RefSeq Size: 5395 bp
• RefSeq ORF: 4077 bp
• Locus ID: 21960
• UniProt ID: Q8BYI9
• Cytogenetics: 1 H1
• MW: 150 kDa
• Gene Summary: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. Theses interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediates inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance (By similarity).[UniProtKB/Swiss-Prot Function]