Clcn6 (NM_011929) Mouse Tagged ORF Clone

CAT#: MR224701

Clcn6 (Myc-DDK-tagged) - Mouse chloride channel 6 (Clcn6)

Product Images

Circular map for MR224701

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clcn6
• Synonyms: AI850629; Clc6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224701 representing NM_011929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGTGCAGGGGGTCCGTGTGCTGCTGCTGCCGGTGGTGTTGCTGCTGCGGTGAACGGGAGAGCC
  GCACCCCCGAGGAGCTGACCATCCTTGGGGAGACACAGGAAGAAGAGGATGAGATTCTTCCAAGGAAAGA
  CTATGAGAGTCTGGACTATGACCGCTGCATCAATGACCCTTACCTGGAGGTGTTGGAGACCATGGATAAT
  AAGAAAGGGCGAAGATACGAGGCGGTGAAGTGGATGGTGGTGTTCGCCATTGGCGTCTGTACGGGGCTGG
  TGGGTCTCTTTGTGGACTTTTCCGTGCGACTCTTCACCCAACTGAAGTTCGGAGTGGTTCAAACGTCGGT
  GGAGGAATGCAGTCAGAAGGGCTGCCTTGCCCTGTCCCTCCTGGAGCTCCTGGGCTTTAACTTGACCTTT
  GTCTTCCTGGCAAGCCTTCTTGTCTTGATAGAGCCAGTGGCAGCAGGTTCTGGAATCCCCGAGATCAAGT
  GCTACCTGAATGGTGTGAAAGTGCCAGGAATTGTCCGGCTCCGGACCCTGCTCTGCAAAGTCTTTGGGGT
  GCTGTTCAGTGTGTCTGGAGGGCTCTTTGTGGGGAAAGAAGGCCCCATGATCCACAGTGGTGCTGTGGTA
  GGAGCTGGCCTCCCTCAGTTCCAGAGCATCTCCTTACGGAAGATCCAGTTTAACTTCCCCTACTTCCGCA
  GCGACAGAGACAAGCGTGACTTTGTGTCAGCGGGGGCAGCTGCTGGCGTTGCTGCAGCTTTTGGGGCCCC
  TATAGGGGGCACCTTGTTCAGTCTGGAGGAGGGTTCATCCTTCTGGAACCAGGGGCTCACGTGGAAAGTG
  CTCTTCTGTTCCATGTCCGCCACCTTCACCCTCAACTTCTTCCGCTCTGGGATTCAGTTTGGAAGTTGGG
  GTTCCTTCCAGCTGCCTGGATTGCTGAACTTTGGTGAATTTAAGTGCTCTGACTCTGATAAAAAATGTCA
  TCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATGGGGGTCATTGGGGGCCTCCTGGGAGCCACA
  TTCAACTGTCTGAACAAGAGGCTTGCAAAGTACCGTATGCGCAACGTGCACCCGAAACCTAAGCTCGTCA
  GAGTCTTAGAGAGCCTCTTGGTGTCCCTGGTGACCACTGTGGTGGTGTTTGTGGCCTCCATGGTGTTAGG
  AGAATGCCGACAGATGTCTTCCACGAGTCAAACCGGTAATGGCTCATTCCAGCTCCAGGTCACATCAGAA
  GATGTGAATTCAACTATCAAGGCTTTTTTCTGTCCCAATGACACTTACAATGACATGGCCACACTCTTCT
  TCAACTCCCAGGAGTCTGCCATCCTGCAGCTCTTCCATCAGGATGGGACTTTCAGCCCCGTCACCCTGGC
  CTTATTCTTCATCCTCTATTTCCTGCTCGCATGCTGGACTTTTGGCACATCTGTCCCCAGTGGCCTCTTC
  GTGCCTTCCCTGCTGTGTGGAGCAGCTTTTGGACGTTTAGTTGCCAATGTCCTGAAAAGCTACATTGGAC
  TGGGCCACCTCTATTCTGGGACCTTTGCCCTGATTGGTGCGGCAGCTTTCTTGGGTGGGGTTGTAAGGAT
  GACCATCAGCCTCACAGTCATCCTGATCGAGTCTACAAATGAGATCACCTATGGGCTTCCGATCATGGTC
  ACTCTGATGGTGGCCAAGTGGACTGGAGACTTGTTCAACAAGGGCATTTATGACGTCCATATTGGGCTGA
  GAGGTGTGCCTCTTCTGGAGTGGGAGACAGACGTGGAAATGGACAAGCTGAGAGCCAGTGACATCATGGA
  GCCCAACCTGACGTACGTCTACCCGCACACTCGCATCCAGTCTCTGGTGAGCATCCTGCGCACTACGGTC
  CACCATGCCTTCCCAGTGGTCACGGAGAACCGGGGCAACGAGAAGGAGTTCATGAAGGGCAACCAGCTCA
  TAAGCAACAACATCAAGTTCAAGAAATCCAGTATCCTGACCCGTGCTGGTGAGCAGCGCAAGCGGGGCCA
  GTCCATGAAGTCGTACCCTTCCAGCGAGCTGCGCAACGTGTGTGATGAGCATGTGGCTTCTGAGGAGCCG
  GCAGAGAAGGAGGACCTGCTACAGCAGATGCTGGAGAGGAGATACACTCCCTACCCCAACCTATACCCTG
  ACCAGTCCCCGAGTGAAGACTGGACCATGGAGGAGCGTTTCCGTCCGCTGACCTTTCACGGCCTTGTCCT
  GCGCTCTCAGCTTGTCACCCTGCTTGTCCGAGGAGTGTGCTATTCGGAAAGTCAGTCCAGTGCCAGCCAA
  CCACGTCTTTCTTACGCTGAGATGGCTGAGGACTACCCGCGATACCCGGACATACATGACCTGGACCTCA
  CACTGCTGAATCCCCGAATGATTGTGGATGTCACCCCATATATGAATCCTTCACCATTTACCGTCTCCCC
  CAACACCCATGTTTCTCAAGTGTTCAACCTGTTCAGAACCATGGGCCTGCGCCACTTGCCTGTGGTGAAT
  GCAGTAGGAGAGATCGTGGGGATCATTACACGGCACAACCTGACAAATGAATTCCTGCAGGCCCGGCTGC
  GGCAGCATTACCAGACCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224701 representing NM_011929
  Red=Cloning site Green=Tags(s)
  
  MAGCRGSVCCCCRWCCCCGERESRTPEELTILGETQEEEDEILPRKDYESLDYDRCINDPYLEVLETMDN
  KKGRRYEAVKWMVVFAIGVCTGLVGLFVDFSVRLFTQLKFGVVQTSVEECSQKGCLALSLLELLGFNLTF
  VFLASLLVLIEPVAAGSGIPEIKCYLNGVKVPGIVRLRTLLCKVFGVLFSVSGGLFVGKEGPMIHSGAVV
  GAGLPQFQSISLRKIQFNFPYFRSDRDKRDFVSAGAAAGVAAAFGAPIGGTLFSLEEGSSFWNQGLTWKV
  LFCSMSATFTLNFFRSGIQFGSWGSFQLPGLLNFGEFKCSDSDKKCHLWTAMDLGFFVVMGVIGGLLGAT
  FNCLNKRLAKYRMRNVHPKPKLVRVLESLLVSLVTTVVVFVASMVLGECRQMSSTSQTGNGSFQLQVTSE
  DVNSTIKAFFCPNDTYNDMATLFFNSQESAILQLFHQDGTFSPVTLALFFILYFLLACWTFGTSVPSGLF
  VPSLLCGAAFGRLVANVLKSYIGLGHLYSGTFALIGAAAFLGGVVRMTISLTVILIESTNEITYGLPIMV
  TLMVAKWTGDLFNKGIYDVHIGLRGVPLLEWETDVEMDKLRASDIMEPNLTYVYPHTRIQSLVSILRTTV
  HHAFPVVTENRGNEKEFMKGNQLISNNIKFKKSSILTRAGEQRKRGQSMKSYPSSELRNVCDEHVASEEP
  AEKEDLLQQMLERRYTPYPNLYPDQSPSEDWTMEERFRPLTFHGLVLRSQLVTLLVRGVCYSESQSSASQ
  PRLSYAEMAEDYPRYPDIHDLDLTLLNPRMIVDVTPYMNPSPFTVSPNTHVSQVFNLFRTMGLRHLPVVN
  AVGEIVGIITRHNLTNEFLQARLRQHYQTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011929
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011929.3, NP_036059.1
• RefSeq Size: 2910 bp
• RefSeq ORF: 2613 bp
• Locus ID: 26372
• UniProt ID: O35454
• Cytogenetics: 4 78.67 cM
• MW: 97.4 kDa
• Gene Summary: This gene encodes a member of the ClC chloride channel and transporter family of proteins. The encoded protein may function as a vesicular Cl-/H+ antiporter. Homozygous knockout mice exhibit decreased pain sensitivity, behavioral abnormalities and features of lysosomal storage disease. [provided by RefSeq, Aug 2015]