Cir1 (NM_025854) Mouse Tagged ORF Clone
CAT#: MR224669
- TrueORF®
Cir1 (Myc-DDK-tagged) - Mouse corepressor interacting with RBPJ, 1 (Cir1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_025854" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cir1 |
Synonyms | 1700023B02Rik; 2810021A19Rik; Cicr; Cir |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224669 representing NM_025854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGTCATTCGCCAATTTCATGTGTAAGAAGGACTTCCATCCTGCGTCCAAATCCAACATCAAGA AGGTATGGATGGCAGAACAGAAAATATCGTATGATAAAAAGAAACAAGAAGAATTAATGCAACAGTACCT TAAAGAGCAGGAGTCGTATGATAACAGATTGCTTATGGGAGATGAACGTGTGAAGAATGGCCTGAATTTC ATGTACGAGGCCCCACCAGGAGTTAAGAAAGAAAACAAAGAGAAAGAAGAAACGGAAGGAGAGACCGAGT ACAAGTTTGAATGGCAGAAAGGCGCTCCGAGAGAAAAATATGCCAAAGATGACATGAACATCAGAGATCA GCCCTTTGGTATTCAGGTTCGAAATGTAAGGTGTATTAAATGTCACAAGTGGGGCCATGTGAACACAGAT CGAGAGTGTCCCTTGTTTGGTCTTTCTGGGATCAATGCAAGTTCAGTTCCTACTGATGGCTCAGGGCCGT CGATGCACCCCTCGGAGCTAATAGCGGAGATGAGAAACAGTGGGTTTGCACTGAAACGAAATGTACTGGG GAGAAACTTGACCGCAAATGATCCATCACAGGACTATGTCGCAAGTGACTGTGAAGAAGACCCTGAAGTT GAGTTCTTAAAGTCGCTCACCACCAAGCAGAAGCAGAAGCTCCTCAGGAAATTGGATAGACTTGAAAAGA AAAAAAAGAAAAAGAAAAGTGATAAGAAAAAGAAGAAGTTACAGAAGAGCAAAAATAAACACAAGAAACG TAAAAACAAATCCCCTTCCTCTTCCTCCTCTTCCTCCTCTTCCTCCTCTTCCTCCTCCTCCTCCTCCTCT TCCTCTTCCTCCTCCTCGGAGACATCAGATAGCAGCAGTGAGAGTGATAACAAAGAAAAGAAAAGAGAGA AAGAGAAGAGGAAGAAGAAGAAGAAAACCAAATGTTCTGAAAGTAAATCCAGTGACTGTAAGGAGGACAA GCCTAAGAACATGCTTTACGAAGAACTTTCCAGCAGTCACAGTGACAGGGGAAAAGCCCAGGAAAAATTA AGGTTTCCAAAACAAGAGAGTTCTGGGGAGAATAGCATGTGGGTCCATTCTGCTTCTGACCGGACATCCA GAAGCCACCGGCACAGTCCAGAGAAGAAAGGGTCTGACAGAAATCGGGGAATCCGAAGTCGGAGCCGTAG CCGGGCTGAGAGCAGCCGGCGAAGCAGAAGCAGGAGTCCTTACCGTCAGAAGCACAGGGAGGTGAGGAGC CGGCCACACCGGAGCCCTAGTGAAGAGCAGAAGGGAAGAAAGGGCACCAGAAGCCATGGTGAAGGTGACC ATAGAAGAGAGCACGTGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224669 representing NM_025854
Red=Cloning site Green=Tags(s) MGKSFANFMCKKDFHPASKSNIKKVWMAEQKISYDKKKQEELMQQYLKEQESYDNRLLMGDERVKNGLNF MYEAPPGVKKENKEKEETEGETEYKFEWQKGAPREKYAKDDMNIRDQPFGIQVRNVRCIKCHKWGHVNTD RECPLFGLSGINASSVPTDGSGPSMHPSELIAEMRNSGFALKRNVLGRNLTANDPSQDYVASDCEEDPEV EFLKSLTTKQKQKLLRKLDRLEKKKKKKKSDKKKKKLQKSKNKHKKRKNKSPSSSSSSSSSSSSSSSSSS SSSSSSETSDSSSESDNKEKKREKEKRKKKKKTKCSESKSSDCKEDKPKNMLYEELSSSHSDRGKAQEKL RFPKQESSGENSMWVHSASDRTSRSHRHSPEKKGSDRNRGIRSRSRSRAESSRRSRSRSPYRQKHREVRS RPHRSPSEEQKGRKGTRSHGEGDHRREHVR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025854 |
ORF Size | 1350 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025854.3, NP_080130.2 |
RefSeq Size | 1945 bp |
RefSeq ORF | 1353 bp |
Locus ID | 66935 |
UniProt ID | Q9DA19 |
Cytogenetics | 2 C3 |
MW | 51.8 kDa |
Gene Summary | Regulates transcription and acts as corepressor for RBPJ. Recruits RBPJ to the Sin3-histone deacetylase complex (HDAC). Required for RBPJ-mediated repression of transcription (By similarity). May modulate splice site selection during alternative splicing of pre-mRNAs.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215996 | Cir1 (untagged) - Mouse corepressor interacting with RBPJ, 1 (Cir1), (10ug) |
USD 503.00 |
|
MG224669 | Cir1 (tGFP-tagged) - Mouse corepressor interacting with RBPJ 1 (Cir1), (10ug) |
USD 657.00 |
|
MR224669L3 | Lenti ORF clone of Cir1 (Myc-DDK-tagged) - Mouse corepressor interacting with RBPJ, 1 (Cir1) |
USD 757.00 |
|
MR224669L4 | Lenti ORF clone of Cir1 (mGFP-tagged) - Mouse corepressor interacting with RBPJ, 1 (Cir1) |
USD 757.00 |
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