Gucy1b1 (NM_017469) Mouse Tagged ORF Clone
CAT#: MR224437
- TrueORF®
Gucy1b3 (Myc-DDK-tagged) - Mouse guanylate cyclase 1, soluble, beta 3 (Gucy1b3), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_017469" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Gucy1b1 |
Synonyms | GC-S-beta-1; GCbeta1; Gucy1b3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224437 representing NM_017469
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGGTTTCGTGAACCATGCCCTGGAGCTGCTGGTGATCCGCAATTATGGTCCCGAGGTGTGGGAAG ACATCAAAAAAGAGGCACAGCTGGATGAAGAAGGCCAGTTTCTTGTCAGAATAATATACGATGATTCCAA AACATATGACCTGGTGGCTGCTGCAAGCAAAGTCCTCAACCTCAATGCTGGCGAAATCCTGCAGATGTTT GGGAAGATGTTTTTCGTCTTCTGCCAGGAGTCTGGCTATGATACCATCTTGCGTGTCCTGGGATCTAATG TCAGAGAGTTTTTGCAGAACCTCGATGCCCTGCATGACCACCTCGCCACCATTTACCCAGGGATGCGCGC GCCTTCCTTCAGGTGCACCGATGCGGAGAAAGGCAAAGGGCTCATCCTGCACTACTACTCGGAAAGAGAG GGGCTTCAGGACATCGTGATCGGGATTATCAAGACTGTTGCTCAACAGATACACGGCACTGAGATAGACA TGAAGGTGATTCAGCAAAGAAATGAAGAATGTGATCACACCCAGTTTTTAATTGAAGAAAAGGAATCAAA AGAAGAGGATTTTTATGAAGATCTGGATAGGTTTGAAGAGAATGGTACCCAGGAATCACGTATCAGCCCT TACACCTTCTGCAAAGCATTTCCTTTTCACATCATATTTGACCGGAACCTAGTGGTCACTCAGTGTGGCA ATGCCATCTACAGAGTGCTCCCCCAGCTCCAGCCTGGGAACTGCAGCCTTCTGTCTGTCTTCTCTCTGGT CCGCCCTCACATCGACATCAGTTTCCATGGGATTCTTTCACACATCAATACAGTCTTTGTACTGAGAAGC AAGGAAGGGTTGCTGGATGTGGAGAAACTTGAGTGTGAGGATGAACTGACTGGAGCAGAGATTAGCTGCT TACGTCTCAAAGGCCAAATGATCTACTTACCAGAAGCAGATAGCATCCTCTTCCTCTGTTCACCAAGTGT GATGAACCTGGACGACCTAACAAGAAGAGGCCTGTATCTGAGTGACATCCCTCTCCACGATGCTACCCGA GACCTGGTTCTTTTGGGAGAACAGTTCCGGGAGGAGTACAAACTGACACAAGAGCTGGAAATCCTCACCG ACAGGCTGCAGCTCACACTGAGAGCCTTGGAGGATGAGAAGAAAAAGACAGACACATTGCTGTATTCTGT CCTTCCTCCATCTGTTGCCAATGAGCTGAGACACAAGCGCCCAGTGCCTGCCAAAAGATACGACAATGTG ACCATCCTCTTCAGCGGCATTGTGGGCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGAGAAGGGGCCA TGAAGATTGTCAATCTCCTCAACGATCTCTACACCCGATTTGACACACTGACTGATTCACGAAAAAACCC ATTTGTTTACAAGGTGGAAACAGTTGGTGACAAGTATATGACAGTGAGTGGCTTGCCAGAACCTTGTATC CACCATGCACGGTCCATTTGCCACCTGGCTTTAGACATGATGGAAATTGCTGGTCAAGTTCAAGTAGATG GTGAATCTGTTCAGATAACAATCGGGATCCATACCGGGGAGGTGGTGACAGGTGTGATTGGACAGCGGAT GCCTCGGTATTGTCTCTTTGGGAATACCGTCAACCTCACAAGCAGGACAGAAACCACAGGAGAAAAGGGA AAGATAAATGTTTCCGAATATACATACAGGTGTCTCATGTCTCCAGAAAACTCGGATCCACTGTTCCATT TGGAGCACAGAGGCCCAGTGTCTATGAAGGGCAAGAAGGAACCAATGCAAGTCTGGTTCCTATCCAGGAA AAATACAGGCACGGAGGAAACAAATGAGGAGGATGAAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224437 representing NM_017469
Red=Cloning site Green=Tags(s) MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMF GKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSERE GLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISP YTFCKAFPFHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRS KEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATR DLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNV TILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCI HHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKG KINVSEYTYRCLMSPENSDPLFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETNEEDEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017469 |
ORF Size | 1860 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017469.4, NP_059497.1 |
RefSeq Size | 3251 bp |
RefSeq ORF | 1863 bp |
Locus ID | 54195 |
UniProt ID | O54865 |
Cytogenetics | 3 E3 |
MW | 71 kDa |
Gene Summary | Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219825 | Gucy1b3 (untagged) - Mouse guanylate cyclase 1, soluble, beta 3 (Gucy1b3), transcript variant 1, (10ug) |
USD 636.00 |
|
MG224437 | Gucy1b3 (tGFP-tagged) - Mouse guanylate cyclase 1 soluble beta 3 (Gucy1b3) transcript variant 1, (10ug) |
USD 835.00 |
|
MR224437L3 | Lenti ORF clone of Gucy1b3 (Myc-DDK-tagged) - Mouse guanylate cyclase 1, soluble, beta 3 (Gucy1b3), transcript variant 1 |
USD 935.00 |
|
MR224437L4 | Lenti ORF clone of Gucy1b3 (mGFP-tagged) - Mouse guanylate cyclase 1, soluble, beta 3 (Gucy1b3), transcript variant 1 |
USD 935.00 |
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