Jade1 (NM_001130186) Mouse Tagged ORF Clone

CAT#: MR224408

Phf17 (Myc-DDK-tagged) - Mouse PHD finger protein 17 (Phf17), transcript variant 4

Product Images

Circular map for MR224408

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Jade1
• Synonyms: AU041499; D530048A03Rik; mKIAA1807; Phf17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224408 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACGAGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCTTGTCAACGACATGGT
  CCCAGCATTCTCGATCCCAGCATGGGAGGAGTAGTACCTGCTCTAGACCTGAAGATCGAAAGCCTTCTGA
  GGTGTTTAGGACCGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAACCCGGATGACTAC
  TACGTGCTGGCTGATCCCTGGAGACAGGAGTGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCAGGGACCA
  TCCCACAGCCTGTGGCCAGGGTTGTGTCTGAAGAGAAGTCTCTCATGTTCATCAGGCCCAAGAAGTACAT
  CGCGTCGTCCGGCTCTGAGCCCCCAGCGTTAGGCTACGTCGATATCCGGACTCTGGCAGACAGTGTGTGT
  CGGTATGACCTCAATGATATGGATGCTGCGTGGCTGGAAGTAACCAATGAAGAATTTAAGGAGATGGGGA
  TGCCGGAGCTGGACGAGTACACCATGGAAAGGGTCTTGGAGGAATTCGAACAGCGATGCTATGACAATAT
  GAATCATGCCATAGAGACAGAAGAGGGCTTGGGGATTGAATACGACGAAGACGTTGTCTGCGATGTCTGC
  CAGTCACCTGATGGCGAGGACGGCAACGAGATGGTGTTCTGTGACAAGTGTAACATCTGTGTGCACCAGG
  CTTGCTATGGGATCCTCAAGGTGCCAGAGGGCAGTTGGCTATGTCGTACATGTGCCCTGGGAGTTCAGCC
  AAAATGTTTGCTGTGTCCCAAGAAAGGCGGAGCTATGAAGCCGACCCGCAGCGGAACCAAGTGGGTCCAC
  GTCAGCTGTGCCCTGTGGATCCCTGAGGTAAGCATTGGCAGCCCTGAGAAGATGGAGCCCATCACAAAAG
  TGTCTCACATTCCCAGCAGCCGGTGGGCGCTTGTGTGCAGCCTCTGCAATGAGAAATTCGGGGCCTCCAT
  ACAGTGCTCTGTGAAGAACTGCCGGACAGCCTTCCACGTGACTTGTGCTTTTGACCGTGGCCTGGAGATG
  AAGACCATATTGGCAGAGAATGATGAAGTCAAATTCAAGTCCTACTGCCCAAAGCACAGCTCACACAGGA
  AACCCGAGGAGGGCCTGGGTGAAGGAGCAGCCCAGGAGAATGGGGCCCCTGAGAGTTCTCCCCAGAGCCC
  TCTGGAGCCCTATGGTAGTCTGGAGCCGAATCGAGAGGAGGCCCACCGGGTGAGTGTTCGCAAGCAGAAG
  CTACAGCAGCTGGAGGATGAGTTCTACACCTTTGTCAACCTGCTGGATGTGGCCAGGGCGCTGCGGCTGC
  CTGAGGAAGTGGTGGATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGATCAACTTCAACAAGCCCCT
  CATCACCCCAAAGAAAGACGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTGTTTAGGAGGCTG
  CAGCTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTTCGGAACCTCACTTACATGGTGACCCGCAGGG
  AAAAGATTAAACGGTCTGTGTGCAAAGTCCAGGAACAAATTTTCACTCAGTACACTAAGCTCTTGGAGCA
  AGAAAAAGTTTCAGGTGTGCCTTCTTCCTGCTCCTCCGCACTGGAGAACATGCTTTTTTTCAACAGTCCT
  TCTGTGGGCCCCAATGCTCCCAAGATAGAGGACTTGAAATGGCATTCTGCATTCTTCAGGAAACAAATGG
  GCACTTCCTTGGTTCATCCACTGAAAAAGTCCCATAAACGAGATGCAGTGCAGAATAGTTCTGGGACCGA
  GGGCAAGACCTCGCATAAGCAGCCAGGTCTTTGTGGTAGAAGGGAGGGGCTGGAGGTCTCAGAAAGCTTG
  CTGAGCTTAGAAAAGACTTTTGCAGAAGCACGTCTCCTATCATCAGCACAACAGAAAAATGGTGTGGTGA
  CCCCAGACCATGGGAAAAGAAGAGACAATCGTTTTCATTGTGATCTCGTTAAAGGAGACTTAAAGGACAA
  ATCTTTTAAGCAGAGTCACAAGCCTCTCAGGTCCACAGACACATCCCAGAGGCATCTGGACAACACAAGA
  GCTGCCACCTCCCCTGGAGTAGGGCAGTCAGCACCTGGCACCAGGAAGGAGATTGTGCCCAAGTGCAATG
  GCTCCCTAGTCAAAGTGCCTATAACACCTGCCAGCCCAGTGAAAAGCTGGGGAGGATTCCGGATTCCAAA
  GAAGGGGGAGCGGCAGCAGCAAGGAGAAGCCCATGATGGGGCCTGCCACCAGCACTCAGACTGCTCCCAT
  CTGGGTGTAAGCCGAGCTCCAGCCAAGGAGAGAGCGAAGAGCAGGTTAAGAGCTGACAGTGAGAATGATG
  GGTACGCCCCTGATGGGGAAATGAGTGACTCAGAGAGCGAGGCATCAGAGAAGAAATGTATCCATGCCAG
  CAGCACCATCAGCAGGAGGACAGATATCATCAGGCGAAGCATCTTGGCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224408 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRGRLPSSSEDSDDNGSLSTTWSQHSRSQHGRSSTCSRPEDRKPSEVFRTDLITAMKLHDSYQLNPDDY
  YVLADPWRQEWEKGVQVPVSPGTIPQPVARVVSEEKSLMFIRPKKYIASSGSEPPALGYVDIRTLADSVC
  RYDLNDMDAAWLEVTNEEFKEMGMPELDEYTMERVLEEFEQRCYDNMNHAIETEEGLGIEYDEDVVCDVC
  QSPDGEDGNEMVFCDKCNICVHQACYGILKVPEGSWLCRTCALGVQPKCLLCPKKGGAMKPTRSGTKWVH
  VSCALWIPEVSIGSPEKMEPITKVSHIPSSRWALVCSLCNEKFGASIQCSVKNCRTAFHVTCAFDRGLEM
  KTILAENDEVKFKSYCPKHSSHRKPEEGLGEGAAQENGAPESSPQSPLEPYGSLEPNREEAHRVSVRKQK
  LQQLEDEFYTFVNLLDVARALRLPEEVVDFLYQYWKLKRKINFNKPLITPKKDEEDNLAKREQDVLFRRL
  QLFTHLRQDLERVRNLTYMVTRREKIKRSVCKVQEQIFTQYTKLLEQEKVSGVPSSCSSALENMLFFNSP
  SVGPNAPKIEDLKWHSAFFRKQMGTSLVHPLKKSHKRDAVQNSSGTEGKTSHKQPGLCGRREGLEVSESL
  LSLEKTFAEARLLSSAQQKNGVVTPDHGKRRDNRFHCDLVKGDLKDKSFKQSHKPLRSTDTSQRHLDNTR
  AATSPGVGQSAPGTRKEIVPKCNGSLVKVPITPASPVKSWGGFRIPKKGERQQQGEAHDGACHQHSDCSH
  LGVSRAPAKERAKSRLRADSENDGYAPDGEMSDSESEASEKKCIHASSTISRRTDIIRRSILAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130186
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130186.1, NP_001123658.1
• RefSeq Size: 5524 bp
• RefSeq ORF: 2505 bp
• Locus ID: 269424
• UniProt ID: Q6ZPI0
• Cytogenetics: 3 B
• MW: 93.9 kDa
• Gene Summary: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function (By similarity).[UniProtKB/Swiss-Prot Function]