Slc45a4 (NM_001168255) Mouse Tagged ORF Clone

CAT#: MR223954

Slc45a4 (Myc-DDK-tagged) - Mouse solute carrier family 45, member 4 (Slc45a4), transcript variant 2

Product Images

Circular map for MR223954

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc45a4
• Synonyms: 9330175B01Rik; AW552276
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223954 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGGCTCCGCAGAATGCCGACTCGGAATCTATGCAAGTACAAGAGCTGCCTGTGCCCCTGCCAG
  ACCCTCAGAAACCCAGAGACCCAGAGGCGGAGACCCAAGAGGAGACCACAAGCGAGGGGTCCATAGACAG
  AATCCCCACACGCCTATGGGTCATGCATGGGGCAGTGATGTTTGGCAGGGAATTTTGTTACGCGATGGAG
  ACAGCACTGGTTACACCTATACTGTTACAGATAGGCCTCCCTGAGAAGTACTACAGCCTGACCTGGTTCC
  TGAGCCCCGTCCTCGGCCTCATCTTCACACCGCTTATTGGCTCTGCAAGTGACCGCTGCACCCTGAGCTG
  GGGCCGCCGGCGGCCCTTCATCCTTGCGCTGTGTGTGGGGGTCCTCATTGGTGTTGCACTTTTCCTCAAC
  GGCTCCGCCATAGGTCTGGCGCTCGGTGATGTTCCCAGCCGGCAGCCCATTGGCATTGTCCTCACGGTGC
  TGGGAGTGGTGGTCCTGGATTTCAGTGCCGATGCAACTGAAGGGCCCATTCGTGCCTACCTGCTGGATGT
  GGTAGACAGTGAGGAGCAAGACATGGCCCTCAACATACACGCCTTCTCTGCTGGCCTTGGTGGGGCCATT
  GGCTACGTGCTGGGTGGGCTGGACTGGACGCAGACCTTCCTAGGTGACTGGTTCCAGACGCAGAACCAGG
  TGCTGTTCTTCTTCGCTGCCGTCATCTTCTCCGTGTCGGTGGCTCTGCATCTCTTCAGCATCGAGGAGGA
  GCAGTACAGCCCACAGCAGGACCGCGGGCCTGAGGATCCCACCCTGCCCGGCACCAGCGTTCAGCCTGGC
  GCCCCAGCCCCTGCCTCCCGCCTCAGCTCCCTTGGTGGGGGCATGCAGGACGGCAGCCCCCCATTCCCAG
  ATGAGGTACAGTCAGAGCACGAGCTGTCCTTGGACTACCTCGATGTGGACATCGTGCGCAGCAAGAGTGA
  CTCGGTGCTGCACATGGCCGACGCCACACTGGACATGGAGCCCCAGCTGCTCTTCCTGCATGACATCGAA
  CCCTCCATCTTCCAAGACGCTTCCTACCCCAGCACCCCCCAGAGCACCAGCCAAGAGCTTCTGAGGGCCA
  AGCTGCCCCGCCTGTCCACGTTCCTCAGGGAGTCCACCAAGGAGGACGACACCTTGCTTGATAATCACTT
  GAATGAAGCTAAAGTCCCAAATGGGAGAGGCTCCCCACCAATCAACTCCCTCAGCCGCTCCAAGGTAGAC
  TTGAAGCCCTCAGTCACATCTGGTTCCATGAGGCGGCGTAGGCACATGTTTCACAGGCAGGCTTCTAGCA
  CCTTCTCCTACTACGGCAAGATTGGATCCCACTGCTACCGATACCGCCGGGCCAACGCAGTGGTCCTCAT
  CAAGCCGTCCCGAAGCATGAGCGACCTGTATGACCTGCAGCAGCGGCAGCGCTCCCGGCACCGCAACCAG
  AGTGGGGCCACTGCCTCCAGCGGGGACACGGAGAGTGAGGAGGGTGAGACAGAGACCACCGTGCGCCTGC
  TGTGGCTGTCCATGCTGAAGATGCCCAAGGAGCTGATGTGGCTCTGCCTCTGTCACCTACTCACTTGGTT
  CTCCGTCATCGCAGAGGCTGTCTTCTATACTGACTTCATGGGCCAGGTTATCTTCAAAGGGAACCCGCAG
  GCCCCCTCCAACTCCACCAAGTGGCATGCCTACAATGCTGGCGTGAAGATGGGCTGCTGGGGCCTGGTCA
  TCTACGCTGCTACTGGTGCTATCTGCTCAGCCTTGCTACAGAAGTACCTGGACAACTATGACCTGAGCAT
  CAGGATCATCTACATGTTGGGGACACTGGGCTTCTCCGTGGGCACCGCTGTGATGGCCATGTTTCCCAAC
  GTCTACGTGGCCATGGTCACCATCAGCACCATGGGTGTTGTCTCGATGAGCATCTCCTACTGCCCCTATG
  CCCTCCTAGGGCACTACCATGACATCAAGGAGTATGTCCACCACAGTCCGGGGAATTCCAAGCGTGGATT
  TGGCATTGACTGCGCCATCCTCTCCTGTCAGGTCTACATCTCCCAGATCCTGGTTGCATCTGCCCTTGGG
  GGCGTGGTCGACGCTGTGAATAGTATCGTTGTCATCCCCATAGTGGCCTCTGTAGGCTCTTTCCTGGGCT
  TCTTGACAGCCACATTCCTGGTCATCTACCCTGAGGTGTCAGAGGAACCTAAGGAGGAACAGAAAGGCCT
  GTCCTCAGGGCCCGCTGGCGAAGGCGAGGGTGGAGCGGGCAGTGAGAAGCCCACTGTACTGAAGCTGTCT
  CGGAAAGGGGGCCTTCGGGGGCTGGTGGAGACGGAGTCCATGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223954 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKMAPQNADSESMQVQELPVPLPDPQKPRDPEAETQEETTSEGSIDRIPTRLWVMHGAVMFGREFCYAME
  TALVTPILLQIGLPEKYYSLTWFLSPVLGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLIGVALFLN
  GSAIGLALGDVPSRQPIGIVLTVLGVVVLDFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAI
  GYVLGGLDWTQTFLGDWFQTQNQVLFFFAAVIFSVSVALHLFSIEEEQYSPQQDRGPEDPTLPGTSVQPG
  APAPASRLSSLGGGMQDGSPPFPDEVQSEHELSLDYLDVDIVRSKSDSVLHMADATLDMEPQLLFLHDIE
  PSIFQDASYPSTPQSTSQELLRAKLPRLSTFLRESTKEDDTLLDNHLNEAKVPNGRGSPPINSLSRSKVD
  LKPSVTSGSMRRRRHMFHRQASSTFSYYGKIGSHCYRYRRANAVVLIKPSRSMSDLYDLQQRQRSRHRNQ
  SGATASSGDTESEEGETETTVRLLWLSMLKMPKELMWLCLCHLLTWFSVIAEAVFYTDFMGQVIFKGNPQ
  APSNSTKWHAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSIRIIYMLGTLGFSVGTAVMAMFPN
  VYVAMVTISTMGVVSMSISYCPYALLGHYHDIKEYVHHSPGNSKRGFGIDCAILSCQVYISQILVASALG
  GVVDAVNSIVVIPIVASVGSFLGFLTATFLVIYPEVSEEPKEEQKGLSSGPAGEGEGGAGSEKPTVLKLS
  RKGGLRGLVETESMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168255
• ORF Size: 2358 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168255.1, NP_001161727.1
• RefSeq Size: 3737 bp
• RefSeq ORF: 1776 bp
• Locus ID: 106068
• UniProt ID: Q0P5V9
• Cytogenetics: 15 D3
• MW: 85.8 kDa