Baz1b (NM_011714) Mouse Tagged ORF Clone

CAT#: MR223820

Baz1b (Myc-DDK-tagged) - Mouse bromodomain adjacent to zinc finger domain, 1B (Baz1b)

Product Images

Circular map for MR223820

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Baz1b
• Synonyms: C87820; Wbscr9; WSTF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223820 representing NM_011714
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCTGCTGGGCCGCAAGCCCTTCCCGCTGGTGAAACCGCTGCCCGGAGAGGAGCCGCTCTTCA
  CCATCCCGCACACTCAGGAGGCCTTCCGCACCCGGGAAGAGTATGAAGCCCGCCTGGAAAGATACAGTGA
  ACGCATTTGGACGTGTAAGAGTACAGGAAGCAGTCAGCTAACACACAAGGAGGCCTGGGAAGAGGAACAG
  GAAGTTGCTGAGCTTTTGAAGGAAGAGTTTCCTAACTGGTATGAGAAGCTTGTTTTGGAAATGGTTCACC
  ATAACACAGCCTCCTTAGAGAAGCTTGTCGACTCTGCCTGGTTAGAGATCATGACCAAATATGCCGTGGG
  GGAAGAGTGTGACTTTGAGGTTGGGAAGGAGAAAATGCTCAAGGTGAAGATAGTGAAGATTCATCCTTTG
  GAGAAAGTTGATGAAGAAGCTGTTGAGAAGAAATCTGATGGGGCCTGTGATTCTCCGTCAAGTGACAAAG
  AAAATTCCAGTCAAATGGCTCAGGACCTCCAGAAGAAGGAGACAGTAGTCAAAGAGGATGAAGGCAGGAG
  AGAGAGTATTAATGACAGAGCGCGTAGGTCTCCACGAAAACTTCCTACTTCATTAAAAAAAGGAGAAAGA
  AAATGGGCTCCTCCAAAATTTCTGCCTCACAAATATGATGTGAAGCTACAAAATGAAGATAAGATCATCA
  GCAATGTGCCAGCAGACAGCTTGATTCGAACGGAGAGACCACCAAATAAGGAGATACTTCGATACTTTAT
  TCGGCATAATGCCTTACGTGCTGGTACTGGTGAGAATGCACCTTGGGTGGTGGAAGATGAATTGGTGAAG
  AAATATTCCCTGCCTAGCAAGTTCAGTGACTTTTTACTTGACCCATATAAGTATATGACTCTCAATCCCT
  CTACCAAGAGGAGGAATACTGGATCTCCAGACAGGAAGCCCTCAAAGAAACCTAAGAGAGACAGTTCTTC
  TCTGAGTTCCCCACTAAACCCTAAGCTGTGGTGTCATGTGCACTTGGAGAAGTCGTTAAATGGCCCACCA
  CTCAAAGTAAAGAACTCAAAGAATTCCAAGTCTCCGGAAGAACATTTGGAAGGGGTGATGAAAATTATGT
  CCCCCAACAACAACAAGCTACACAGCTTCCACATTCCTAAGAAAGGCCCAGCCGCCAAGAAGCCAGGGAA
  GCACAGTGACAAACCTCTGAAGGCCAAGGGCAGAGGCAAAGGCATCCTGAATGGACAGAAGTCCACAGGC
  AACTCCAAATCACCCAGCAAGTGTGTGAAGACTCCTAAGACCAAAATGAAGCAGATGACTTTGTTGGATA
  TGGCCAAAGGGACTCAGAAGATGACCCGCACCCCACGGAGTTCTGGGGGTGTGCCAAGGTCTTCTGGTAA
  ACCTCACAAACACCTGCCTCCTGCAGCTCTGCATCTGATTGCCTATTACAAAGAAAATAAAGACAAAGAG
  GACAAGAAGAGTGCTCTGTCCTGTGTTATCTCCAAAACAGCACGTCTCCTCTCCAATGAAGATAGAGCCC
  GTCTTCCAGAAGAGCTCCGGGCTCTTGTCCAGAAACGCTATGAGCTCCTGGAACATAAAAAGAGGTGGGC
  CTCTATGTCTGAAGAGCAACGGAAAGAATATTTGAAAAAGAAACGACAGGAGCTAAAGGAGAGGTTGCGG
  GAAAAAGCCAAAGAACGGAGAGAGAGAGAAATGCTGGAGAGGTTAGAAAAGCAGAAGCGATTTGAGGACC
  AGGAGTTAGGTGGCAGAAACCTCCCAGCATTCAGGCTGGTAGATACCCCTGAGGGGCTCCCCAACACTCT
  TTTTGGGGATGTGGCCCTGGTGGTGGAGTTCCTGAGCTGTTACTCTGGGTTGCTCTTGCCCGATGCTCAG
  TACCCTATCACTGCTGTGTCCCTAATGGAAGCCTTGAGTGCAGATAAAGGTGGTTTTTTGTACTTGAACA
  GAGTGCTGGTCATCCTGCTACAGACCTTACTTCAGGATGAAATTGCAGAAGACTATGGAGAACTGGGAAT
  GAAGCTGTCAGAAATCCCCCTCACCCTGCATTCTGTCTCAGAGCTGGTCCGGCTCTGCTTGCGCAGGTGT
  GATGTCCAGGAGGACAGCGAGGGCTCAGAAACAGATGACAATAAAGATTCCACACCATTTGAGGATAATG
  AGGTGCAAGACGAATTTCTAGAGAAGCTGGAGACATCAGAATTCTTTGAGCTGACCTCAGAGGAGAAGCT
  ACGGATATTGACAGCTCTTTGCCACCGTATCCTCATGACATACTCTGTGCAAGACCACATGGAGACAAGA
  CAGCAGGTGTCTGCAGAGTTGTGGAAGGAGCGGCTTGCTGTGCTGAAGGAGGAGAATGATAAGAAGAGAG
  CAGAGAAGCAAAAGCGGAAGGAGATGGAAGCCAGAAATAAAGAAAATGGCAAAGAGGAGAATGTGCTAGG
  CAAAGTGGACAGGAAAAAAGAAATTGTGAAGATTGAACAGCAAGTAGAAGTGGAAGCAGATGACATGATC
  AGTGCTGTCAAGAGCAGAAGGCTGCTTAGCATGCAAGCTAAGAGGAAGCGGGAAATCCAGGAGAGAGAAA
  CAAAAGTGAGATTGGAACGGGAAGCTGAAGAGGAACGAATGCGGAAACACAAGGCTGCTGCCGAGAAGGC
  TTTCCAGGAAGGGATTGCCAAGGCAAAACTTGTACTGCGCAGGACACCTATTGGTACAGACCGCAACCAT
  AACAGATACTGGCTCTTCTCAAACGAAGTCCCAGGATTATTCATTGAAAAGGGCTGGGTCCATAATAGCA
  TTGACTACCGGTTTAAGCATCACCGCAAAGACCACAGCAATTTACCTGATGATGACTACTGTCCTCGAAG
  TAAGAAAGCAAACCTAGGCAAGAATGCAAGTGTGAATGCGCACCATGGACCAGCACTAGAAGCTGTGGAG
  ACCACTGTGCCCAAGCAAGGACAAAACTTATGGTTCCTGTGTGACAGTCAGAAGGAATTGGATGAGCTGC
  TGAGTTGCCTCCATCCTCAGGGAATCAGGGAAAGTCAGCTTAAAGAGAGGCTGGAGAAGAGATACCAAGA
  AATTACTCATTCTATTTATCTGGCTCGGAAGCCAAATTTGGGTCTAAAATCTTGTGACGGCAACCAGGAG
  CTCTTAAACTTCCTGAGGAGTGATCTTATTGAAGTGGCAACGAGGCTGCAGAAAGGAGGACTTGGCTACA
  TGGAGGGAACATCTGAGTTTGAGGCCCGGGTCATTTCCTTAGAGAAACTGAAGGATTTTGGTGAATGCGT
  CATTGCCCTTCAAGCCAGTGTCATAAAGAAATTTCTGCAAGGATTTATGGCGCCTAAGCAGAAGAAGCGG
  AAACTCCAAAGTGAAGATTCCACAAAGTCCGAGGAAGTAGATGAAGAGAAAAAGATGGTAGAGGAAGCAA
  AGGTTGCCTCTGCACTGGAAAAATGGAAAACAGCAATCCGTGAAGCACAGACCTTTTCCAGGATGCATGT
  TCTACTTGGGATGCTCGATGCATGTATCAAGTGGGACATGTCAGCAGAAAACGCTAGGTGCAAAGTTTGT
  CGAAAGAAAGGTGAGGATGACAAGCTGATCCTGTGTGACGAGTGTAATAAAGCCTTCCACCTGTTCTGTC
  TGAGGCCGGCCCTTTATGAAGTACCAGATGGCGAGTGGCAGTGTCCAGCTTGCCAGCCTCCTACTGCCAG
  ACGCAATTCCCGTGGCAGGAATTACACTGAAGAGTCTACCTCTGAGGGCAGCGAGGGTGATGAGAGCGGT
  GAAGAGGAAGAGGAAGAAGAGGAGGAGGAGGAAGAAGAAGAGGATTATGAAGTGGCTGGCTTGCGGCTGA
  GACCTCGGAAGACCATTCGAGGCAAGCAGAGTGTCATCCCTGCAGCAAGACCAGGCCGACCGCCGGGTAA
  GAAGTCACATCCTGCCAGAAGGTCACGGCCAAAGGATGACCCAGAGGTCGATGATCTGGTACTTCAGACC
  AAGCGTATCTCGAGAAGGCAGAGTCTGGAGCTACAAAAGTGTGAGGATATCCTTCACAAACTGGTGAAGT
  ACCGCTTCAGCTGGCCCTTCCGGGAGCCTGTGACCAGAGACGAGGCTGAGGACTACTATGATGTGATCGA
  GCACCCCATGGACTTCCAGACCATACAGAACAAATGCTCATGCGGGAACTACCGATCTGTGCAGGAGTTC
  CTCACTGACATGAAGCAAGTGTTTGCCAATGCCGAGCTCTATAACTGCCGCGGCAGCCACGTGCTGAGTT
  GCATGGAGAAGACAGAGCAGTGTCTGCTGGCTCTGCTGCAAAAACACCTCCCCGGCCACCCCTACGTGCG
  CAGGAAACGCAGGAAGTTTCCTGATAGGCTCGCTGATGATGAAGGGGACAGTGACTCAGAGTCTGTTGGA
  CAGTCCAGGGGACGGAGACAGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223820 representing NM_011714
  Red=Cloning site Green=Tags(s)
  
  MAPLLGRKPFPLVKPLPGEEPLFTIPHTQEAFRTREEYEARLERYSERIWTCKSTGSSQLTHKEAWEEEQ
  EVAELLKEEFPNWYEKLVLEMVHHNTASLEKLVDSAWLEIMTKYAVGEECDFEVGKEKMLKVKIVKIHPL
  EKVDEEAVEKKSDGACDSPSSDKENSSQMAQDLQKKETVVKEDEGRRESINDRARRSPRKLPTSLKKGER
  KWAPPKFLPHKYDVKLQNEDKIISNVPADSLIRTERPPNKEILRYFIRHNALRAGTGENAPWVVEDELVK
  KYSLPSKFSDFLLDPYKYMTLNPSTKRRNTGSPDRKPSKKPKRDSSSLSSPLNPKLWCHVHLEKSLNGPP
  LKVKNSKNSKSPEEHLEGVMKIMSPNNNKLHSFHIPKKGPAAKKPGKHSDKPLKAKGRGKGILNGQKSTG
  NSKSPSKCVKTPKTKMKQMTLLDMAKGTQKMTRTPRSSGGVPRSSGKPHKHLPPAALHLIAYYKENKDKE
  DKKSALSCVISKTARLLSNEDRARLPEELRALVQKRYELLEHKKRWASMSEEQRKEYLKKKRQELKERLR
  EKAKERREREMLERLEKQKRFEDQELGGRNLPAFRLVDTPEGLPNTLFGDVALVVEFLSCYSGLLLPDAQ
  YPITAVSLMEALSADKGGFLYLNRVLVILLQTLLQDEIAEDYGELGMKLSEIPLTLHSVSELVRLCLRRC
  DVQEDSEGSETDDNKDSTPFEDNEVQDEFLEKLETSEFFELTSEEKLRILTALCHRILMTYSVQDHMETR
  QQVSAELWKERLAVLKEENDKKRAEKQKRKEMEARNKENGKEENVLGKVDRKKEIVKIEQQVEVEADDMI
  SAVKSRRLLSMQAKRKREIQERETKVRLEREAEEERMRKHKAAAEKAFQEGIAKAKLVLRRTPIGTDRNH
  NRYWLFSNEVPGLFIEKGWVHNSIDYRFKHHRKDHSNLPDDDYCPRSKKANLGKNASVNAHHGPALEAVE
  TTVPKQGQNLWFLCDSQKELDELLSCLHPQGIRESQLKERLEKRYQEITHSIYLARKPNLGLKSCDGNQE
  LLNFLRSDLIEVATRLQKGGLGYMEGTSEFEARVISLEKLKDFGECVIALQASVIKKFLQGFMAPKQKKR
  KLQSEDSTKSEEVDEEKKMVEEAKVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVC
  RKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPPTARRNSRGRNYTEESTSEGSEGDESG
  EEEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKQSVIPAARPGRPPGKKSHPARRSRPKDDPEVDDLVLQT
  KRISRRQSLELQKCEDILHKLVKYRFSWPFREPVTRDEAEDYYDVIEHPMDFQTIQNKCSCGNYRSVQEF
  LTDMKQVFANAELYNCRGSHVLSCMEKTEQCLLALLQKHLPGHPYVRRKRRKFPDRLADDEGDSDSESVG
  QSRGRRQKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011714
• ORF Size: 4437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011714.2, NP_035844.2
• RefSeq Size: 6446 bp
• RefSeq ORF: 4440 bp
• Locus ID: 22385
• UniProt ID: Q9Z277
• Cytogenetics: 5 G2
• MW: 171.1 kDa
• Gene Summary: Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication.[UniProtKB/Swiss-Prot Function]