Adgre5 (NM_001163030) Mouse Tagged ORF Clone

CAT#: MR223738

Cd97 (Myc-DDK-tagged) - Mouse CD97 antigen (Cd97), transcript variant 3

Product Images

Circular map for MR223738

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adgre5
• Synonyms: AA409984; Cd97; TM7LN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223738 representing NM_001163030
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGCGTCAGATGCCCCGGCCTGCTTGTGGTGTGCATTTTACTGAGTCTCTCAGGAGCTGGAACCC
  AGAAGGCAGAGAGTAAGAACTGTGCCAAGTGGTGCCCTATCAACTCAAAATGTGTCAGTAACAGAAGCTG
  TGTCTGCAAGCCCGGCTTCAGCTCGGAGAAGGAATTGATCACTAACCCTGCAGAGAGCTGTGAAGACATC
  AACGAGTGTTTACTACCTGGATTTTCCTGTGGAGACTTTGCCATGTGTAAGAACTCAGAGGGGAGTTACA
  CCTGCGTCTGTAACCTGGGATATAAGCTCCTTTCTGGTGCAGAATCTTTTGTTAACGAGAGTGAGAATAC
  ATGTCAAGATGTGAATGAGTGCATCTCCGGGCAAAACCATTGCCACCAATCCACCCACTGCATCAACAAA
  TTGGGTGGCTATTCTTGCATCTGTCGTCAAGGCTGGAAACCTGTTCCTGGATCCCCCAATGGCCCTGTCA
  GCACAGTGTGCGAAGATGTGGATGAGTGCAGCTCTGGGCAGCATCAATGTCACAATTCCACCGTCTGCAA
  AAACACCGTAGGCTCCTACAAGTGCCACTGCCGTCCAGGTTGGAAGCCAACTTCTGGGTCCCTCCGTGGT
  CCAGACACCATATGCCAAGAGCCACCGTTCCCTACTTGGACACTGCTGCCCACAGCCCACAGTCAGACTC
  TCTTGAGATTCTCTGTTGAAGTCCAGAATCTGCTCCGTGACTTCAATCCAGCCACGGTCAACTACACCAT
  CCAGAAACTCATCGAGGCTGTGGACAAGCTGCTGGAAGACCCCATGGAGACTGAAACCCAACAAGTAGCT
  GCCCAGTTGCTCTCGAACCTGGAACAAAGCCTTCGGACCTTGGCTCAGTTTTTACCCAAAGGCCCCTTCA
  CCTACACGTCCCCTTCAAATACTGAACTGTCCCTGATGGTGAAGGAGCAAGACAACAAAGATGTTACCAC
  AGTGCACCACGGCCAAACTTGGATGGAGCTGGACTGGGCTGTGACAGCTGGAGCCAAAATCTCAGAAAAT
  GGCTCCTCGGTGGCAGGCATCCTGTCCAGCCCAAACATGGAAAAGCTGCTGGGCAACACCCCGCTGAACT
  TGGAGCAGAGAAGGGCCTCCTTGGAGGACTTCTATGGAAGCCCGATTCCGAGTGTCTCACTTAAGCTTCT
  CTCAAACATCAATTCTGTCTTCCTGACCAACACGAACACTGAAAAGCTCGCCTCGAATGTCACGTTCAAG
  TTCGACTTTACTTCTGTGGAGTCAATTGAGCCACGTCATGAGCTGATATGTGCCTTCTGGAAAGCCCACA
  ACGGGAATGGGTACTGGGACACCGACGGCTGCTCAATGAATGGCACTGGCTTCTGCCACTGCAACCACCT
  GACCAGCTTTGCCATCCTAATGGCTCAGTACCATGTGCAGGACCCAAGGCTGGAATTGATCACCAAGGTG
  GGGCTGTTGTTGTCCCTGATCTGCCTGCTGCTGTGCATCCTGACCTTCCTGCTGGTGAAGCCCATCCAGA
  GCTCTCGAACCATGGTGCACCTGCACCTGTGCATCTGCCTCTTCCTGGGCTCAATCATATTCCTGGTCGG
  CGTGGAGAATGAAGGGGGTGAGGTGGGCCTGCGCTGCCGCCTGGTGGCCGTGATGCTGCATTTCTGCTTC
  CTGGCCGCCTTCTGCTGGATGGCACTGGAGGGCGTGGAGCTCTACTTCCTGGTGGTGCGTGTGTTCCAGG
  GCCAAGGCCTGAGTACATGGCAACGCTGCCTGATTGGCTACGGGGTGCCCCTCCTCATCGTGGCCATCTC
  AATGGCAGTTGTCAAAATGGATGGCTATGGGCATGCAACATACTGCTGGCTGGACTTTAGGAAGCAGGGC
  TTTCTCTGGAGCTTTTCGGGACCCGTGGCCTTCATCATTTTCTGCAATGCTGCCATTTTCGTGATCACTG
  TCTGGAAGCTCACAAAGAAGTTTTCTGAAATCAACCCAAACATGAAGAAGTTAAGGAAGGCGAGGGTGCT
  GACCATCACCTCCATCGCCCAGCTCCTTGTGCTGGGCTGCACCTGGGGCTTCGGCCTGTTCCTCTTCAAC
  CCCCATAGCACATGGCTATCCTACATCTTTACCCTGCTCAACTGCCTGCAGGGCCTATTCCTCTATGTGA
  TGCTCTGCCTGCTCAACAAAAAGGTGAGGGAAGAGTACTGGAAATGGGCCTGCATGGTCACTGGGAGCAA
  GTACACAGAGTTCAACTCTTCCACAACAGGCACTGGCACCAGCCAAACACGGGCTCTCAGGTCCTCAGAA
  TCAGGGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223738 representing NM_001163030
  Red=Cloning site Green=Tags(s)
  
  MRGVRCPGLLVVCILLSLSGAGTQKAESKNCAKWCPINSKCVSNRSCVCKPGFSSEKELITNPAESCEDI
  NECLLPGFSCGDFAMCKNSEGSYTCVCNLGYKLLSGAESFVNESENTCQDVNECISGQNHCHQSTHCINK
  LGGYSCICRQGWKPVPGSPNGPVSTVCEDVDECSSGQHQCHNSTVCKNTVGSYKCHCRPGWKPTSGSLRG
  PDTICQEPPFPTWTLLPTAHSQTLLRFSVEVQNLLRDFNPATVNYTIQKLIEAVDKLLEDPMETETQQVA
  AQLLSNLEQSLRTLAQFLPKGPFTYTSPSNTELSLMVKEQDNKDVTTVHHGQTWMELDWAVTAGAKISEN
  GSSVAGILSSPNMEKLLGNTPLNLEQRRASLEDFYGSPIPSVSLKLLSNINSVFLTNTNTEKLASNVTFK
  FDFTSVESIEPRHELICAFWKAHNGNGYWDTDGCSMNGTGFCHCNHLTSFAILMAQYHVQDPRLELITKV
  GLLLSLICLLLCILTFLLVKPIQSSRTMVHLHLCICLFLGSIIFLVGVENEGGEVGLRCRLVAVMLHFCF
  LAAFCWMALEGVELYFLVVRVFQGQGLSTWQRCLIGYGVPLLIVAISMAVVKMDGYGHATYCWLDFRKQG
  FLWSFSGPVAFIIFCNAAIFVITVWKLTKKFSEINPNMKKLRKARVLTITSIAQLLVLGCTWGFGLFLFN
  PHSTWLSYIFTLLNCLQGLFLYVMLCLLNKKVREEYWKWACMVTGSKYTEFNSSTTGTGTSQTRALRSSE
  SGM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163030
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163030.1, NP_001156502.1
• RefSeq Size: 3118 bp
• RefSeq ORF: 2322 bp
• Locus ID: 26364
• Cytogenetics: 8 40.22 cM
• MW: 86.1 kDa
• Gene Summary: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.[UniProtKB/Swiss-Prot Function]