Rimbp2 (NM_001081388) Mouse Tagged ORF Clone

CAT#: MR223463

Rimbp2 (Myc-DDK-tagged) - Mouse RIMS binding protein 2 (Rimbp2)

Product Images

Circular map for MR223463

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rimbp2
• Synonyms: A930033C01Rik; mKIAA0318
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223463 representing NM_001081388
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGAGGCTGCTGAGCGGCGGCAACAGCTGGAGTTGGAGCATGAGCAAGCCCTGGCCTTCCTCAATG
  CCAAGCAGCAGGAGATCCAGCTACTGCAGCAGGCTCAGGTTGAAGCTAAGAAAGAGCATGAAGGTGCTGT
  GCAGCTGTTAGAGAACACCCTGGACTGCATGCAGTCCAAGGTCCGAGAGCTGGAAGAGAAATGCCGTGTG
  CAGAGTGAGCAGTTTAACTTGTTGTCCCGGGACCTGGAAAAGTTCCGGCAACACACTGGGAGTATTGACC
  TGCTGGGCAGCAGCTCAGTGGCCCTGCTGGATGTTCCCTTGGCCCCTGGCAAGCCTTTCCCTCAGTACAT
  GAATGGACTAGCCACCTCCATCCACAAAGGTCACGAGGGCCCCACTGGACACTACTCTGTGATTGGTGAC
  TATATTCCGCTGTCTGGGGACAAGCTGGAGTCTCCGTGTGTGAAGCCCTCCTTCCTCTTGCGATCCAGCA
  GCCCAAGATGCAGATTTGAGTCCGAGATGGACAATGACCGGAACTCTAACAACTCCAAGCAAAGCAGCTC
  GGGGAAGGTGCACCTGTGTGTGGCCCGCTACAGTTACAACCCCTTCGATGGGCCCAATGAGAACCCAGAA
  GCTGAGCTGCCTCTCACGGCGGGAAAGTACCTCTATGTCTATGGGGACATGGATGAAGATGGGTTCTATG
  AAGGAGAGCTTCTGGATGGGCAAAGGGGCTTGGTGCCCTCCAACTTTGTGGATTTTATCCAGGACAATGA
  GTCGCGGCTGGCTGGTACTCTGGGGAGTGAGCAGGACCAGAACTTTCTCAACCACTCTGGCATCAGTCTG
  GAGCGCGACAGCATCCTTCACCTTCACTCCCCAACTCAAGTGGACTCGGGGATCACCGACAATGGTGGGG
  GGACCCTGGACGTGAACATCGACGACATCGGAGAAGACACCGTGCCTTACCCTAGGAAAATCACCCTTAT
  CAAACAGCTTGCCAAAAGTGTCATCGTGGGCTGGGAGCCCCCTGCTGTGCCTCCCGGCTGGGGCACCGTG
  AGCAGTTATAATGTGCTGGTGGACAAAGAGACACGCATGAGCCTTGCCCTGGGTAGGAGAACTAAGGCGC
  TGATCGAGAAACTTAACACAGCTGCCTGCACCTACCGCATCTCCGTGCAGTGCGTCACGAGCCGGGGAAA
  CTCAGACGAGCTGCAGTGCACTCTGCTGGTGGGCAAGGATGTGGTGGTGGCACCGTCCCAGCTGCGTGTG
  GACAACATCACACAGATCTCTGCCCAGCTCTCGTGGCTGCCGACCAACAGTAACTACAGCCATATCATCT
  TCCTCAACGAAGAAGAACTGGACATCGTGAAGGCAGCCAGGTACAAGTACCAATTCTTCAACCTCAGGCC
  TAATATGGCCTACAAGGTGAAGGTCTTGGCCCAGCCACACCAGATGCCCTGGCAGCTGCCGCTGGAGCAG
  AGAGAAAAGAAGGAGGCCTGTGTGGAGTTCTCCACGCTGCCTGCAGGACCTCCAGCCCCACCACAAGATG
  TCACTGTCCACGCTGGGGCCACAGCCGCCTCTGTTCAGGTCTCCTGGAAGCCCCCTGCACTGACTCCCAC
  TGGGTTGTCCAATGGAGCAAATGTCACAGGATACGGCGTGTACGCCAAAGGGCAGAGGGTGGCTGAGGTC
  ATCGCCCCCACGGCAGATGGCACGGCAGTGGAGCTGATCCGGCTGCGAAGCCTAGAGGCCAAGGCCGTGA
  GTGTGCGTACCCTGTCTGTACAGGGAGAGTCCATGGATTCTGCCCTCGCTGCCATCCCCCCTGACCTCCT
  GGTGCCTCCAGCCCCCCACCCGAGGACTGCTCCCCCACCAAAGCCATTAGCAAGTGACATGGATACCAAA
  GACCAACACCTGGGGCCCCACGTCAAAGTGGATGAGTCCTGGGAGCAGAGCCGGTCACCGGGCCCTGCAC
  ATGGCCACATGTTGGAACCACCTGACATGCACAGCGCTGGCCCAGGCAGAAGGTCACCCTCGCCCAGCCG
  GATCCTTCCTCAGCCACAAGGAGCCCCCGTGTCTACCACTGTCGCCAAGGCCATGGCCCGTGAAGCTGCA
  CAGAGGGTGGCTGAGAGCAACAGGTTAGAGAAAAGGAGCCTCTTTCTAGAGCAAAGCAGTGCGGGGCAAT
  ATACCAACTCGGACGAGGAGGACGGCTACGCCTCCCCCGAGGTCAAGAGGAGAGGCACCTCAGTGGATGA
  CTTCCTCAAAGGGTCAGAGCTGGGCAAGCAGCCCCACTGTTGCCATGGAGATGAGTACCACACAGAGAGC
  AGCCGGGGGTCAGACCTGTCGGACATCATGGAGGAGGATGAGGAGGAGCTATACTCAGAGATGCAGCTGG
  AGGATGGGGGCCGCCGTCGGCCCAGCGGTACCTCTCACAACGCCCTCAAGATTTTAGGAAACTCCACGTT
  GATGGGACGAGCAGACCGGATGGAACACGTGAGCCGAAGGTATTCACACAGTGGCGGAGGGTCTCATAGG
  CACCGCCCAGCGATGGCTCCATCCATTGATGAATACACCGGGCGAGACCATCTTTCTCCAGACTTCTATG
  ATGAGTCCGAAACTGACCCTGGTGCTGAGGAGCTCCCAGCCCGTATCTTTGTGGCTCTGTTTGACTATGA
  CCCACTGACCATGTCCCCAAACCCAGACGCTGCTGAAGAGGAGCTTCCCTTCAAAGAAGGACAGATCATC
  AAGGTATATGGAGACAAAGACGCAGATGGCTTCTACCGTGGGGAGACCTGTGCCAGGCTCGGCCTCATTC
  CCTGTAACATGGTCTCTGAGATCCATGCGGATGATGAGGAGATGATGGATCAGCTGCTGAGGCAAGGCTT
  CCTCCCTCTGAACACGCCTGTGGAGAAAATAGAGAGAAGTAGAAGAAGCGGCCGGGGTCACTCTGTACCC
  ACACGAAGAATGGTGGCTCTCTACGACTATGATCCTAGGGAAAGCTCTCCTAACGTGGATGTTGAGGCTG
  AACTTCCATTTTGCACAGGAGACATTATTACTGTTTTTGGTGAAATCGATGAAGATGGATTTTATTATGG
  AGAGCTGAATGGGCAAAAAGGCCTCGTGCCTTCCAATTTCCTGGAAGAAGTGCCTGATGATGTGGAGGTC
  CACCTTTCTGATGCTCCGCCCCACTACTCCCACGACCCGCCCATGCGCTCCAAGGCCAAAAGGAAGAAGA
  GTGTTCATTTCACACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223463 representing NM_001081388
  Red=Cloning site Green=Tags(s)
  
  MREAAERRQQLELEHEQALAFLNAKQQEIQLLQQAQVEAKKEHEGAVQLLENTLDCMQSKVRELEEKCRV
  QSEQFNLLSRDLEKFRQHTGSIDLLGSSSVALLDVPLAPGKPFPQYMNGLATSIHKGHEGPTGHYSVIGD
  YIPLSGDKLESPCVKPSFLLRSSSPRCRFESEMDNDRNSNNSKQSSSGKVHLCVARYSYNPFDGPNENPE
  AELPLTAGKYLYVYGDMDEDGFYEGELLDGQRGLVPSNFVDFIQDNESRLAGTLGSEQDQNFLNHSGISL
  ERDSILHLHSPTQVDSGITDNGGGTLDVNIDDIGEDTVPYPRKITLIKQLAKSVIVGWEPPAVPPGWGTV
  SSYNVLVDKETRMSLALGRRTKALIEKLNTAACTYRISVQCVTSRGNSDELQCTLLVGKDVVVAPSQLRV
  DNITQISAQLSWLPTNSNYSHIIFLNEEELDIVKAARYKYQFFNLRPNMAYKVKVLAQPHQMPWQLPLEQ
  REKKEACVEFSTLPAGPPAPPQDVTVHAGATAASVQVSWKPPALTPTGLSNGANVTGYGVYAKGQRVAEV
  IAPTADGTAVELIRLRSLEAKAVSVRTLSVQGESMDSALAAIPPDLLVPPAPHPRTAPPPKPLASDMDTK
  DQHLGPHVKVDESWEQSRSPGPAHGHMLEPPDMHSAGPGRRSPSPSRILPQPQGAPVSTTVAKAMAREAA
  QRVAESNRLEKRSLFLEQSSAGQYTNSDEEDGYASPEVKRRGTSVDDFLKGSELGKQPHCCHGDEYHTES
  SRGSDLSDIMEEDEEELYSEMQLEDGGRRRPSGTSHNALKILGNSTLMGRADRMEHVSRRYSHSGGGSHR
  HRPAMAPSIDEYTGRDHLSPDFYDESETDPGAEELPARIFVALFDYDPLTMSPNPDAAEEELPFKEGQII
  KVYGDKDADGFYRGETCARLGLIPCNMVSEIHADDEEMMDQLLRQGFLPLNTPVEKIERSRRSGRGHSVP
  TRRMVALYDYDPRESSPNVDVEAELPFCTGDIITVFGEIDEDGFYYGELNGQKGLVPSNFLEEVPDDVEV
  HLSDAPPHYSHDPPMRSKAKRKKSVHFTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081388
• ORF Size: 3237 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081388.2, NP_001074857.1
• RefSeq Size: 7020 bp
• RefSeq ORF: 3240 bp
• Locus ID: 231760
• UniProt ID: Q80U40
• Cytogenetics: 5 G1.3
• MW: 119.1 kDa
• Gene Summary: Plays a role in the synaptic transmission as bifunctional linker that interacts simultaneously with RIMS1, RIMS2, CACNA1D and CACNA1B.[UniProtKB/Swiss-Prot Function]