Phf21a (NM_001109691) Mouse Tagged ORF Clone

CAT#: MR223342

Phf21a (Myc-DDK-tagged) - Mouse PHD finger protein 21A (Phf21a), transcript variant 3

Product Images

Circular map for MR223342

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phf21a
• Synonyms: 80kDa; Bhc; Bhc80; D030065N23Rik; PFTF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223342 representing NM_001109691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGCAGACTCTACAGGAGGCTCTTAAAGTGGAAATTCAGGTTCACCAGAAACTGGTTGCCCAAA
  TGAAGCAGGATCCACAGAATGCTGACTTAAAGAAACAGCTTCATGAACTCCAAGCCAAAATCACAGCTTT
  GAGTGAGAAACAGAAAAGAGTAGTTGAGCAGCTGCGCAAGAACCTGATAGTCAAACAAGAGCAGCCGGAC
  AAGTTCCAGATACAGCCATTGTCACAGTCTGAAAACAAACTACAAACAGCACAGCAGCAGCCACTACAGC
  CACTGCAGCAGCAGCAACCGCAGCAACCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACGCCCAGCAGTC
  AGCAGCAGCACCGCCTAGTCTAACTGCATCACAGCAGGTTCAAGCAACACCTCCTCAGCCCATCAAAGTA
  CCACAGTTTATCCCTCCTCCTAGACTCACTCCACGCCCAAACTTCCTTCCACAGGTTCGACCCAAGCCTG
  TGGCCCAGAATAACATTCCTATCGCTCCAGCGCCTCCTCCCATGCTTGCAGCTCCTCAGCTTATCCAGAG
  GCCTGTCATGCTGACCAAGTTTACACCCACAACCCTCCCCACATCCCAGAATTCCATCCACCCTGTCCGT
  GTTGTCAATGGACAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGCTCACCAGCATTGTCATAG
  CTACTCCAGGGACCAGACTCCCTGGACCTCAGACTGTACAGCTTAGCAAACCAAGCCTGGAGAAACAGAC
  AGTTAAATCCCACCCAGAAGCAGAGGAAAAACAAGCAGAGAGCCGTACCGTTACCCCACCTGCTGCACCA
  AAACCAAAACGAGAAGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAGGGTTGGTAACACATGACC
  ATCTAGAAGAAATCCAAAGCAAGAGGCAGGAAAGAAAGAGAAGAACAACAGCAAACCCGGTGTACAGTGG
  GGCTGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTCACTTACCTTAACAGCACAATGCATCCTGGGACG
  CGGAAGAGAGGTCGTCCTCCAAAATACAATGCGGTGCTGGGGTTTGGAGCCCTTACCCCAACATCCCCCC
  CATCCAGTCATCCCGACTCCCCTGAAAATGAAAAGACAGAGACCACATTCACTTTCCCTGCACCTGTTCA
  GCCTGTGTCTTTGCCCAGCCCCACCTCCACAGACGGTGATATCCATGAGGATTTTTGCAGTGTTTGCAGA
  AAAAGTGGCCAGTTGCTGATGTGTGACACGTGTTCCCGTGTGTATCATCTGGACTGCTTAGAGCCGCCCC
  TGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATGCTAAAGAAGGAAGAAGC
  AATTCCTTGGCCTGGAACCTTAGCCATTGTTCATTCCTACATCGCCTACAAAGCAGCAAAAGAAGAGGAG
  AAACAGAAGCTACTTAAGTGGAGTTCAGATTTAAAACAGGAGCGGGAACAACTGGAGCAGAAGGTCAAAG
  AGCTCAGCAGTTCTATAAGTAAATGTATGGAGATGAAGAGCAGCATCCTGGCGCGGCAGAAGGAGATGCG
  CAGCTCCCTGGACAAGGTGAAGCGGCTCATCCGCCTTGTGCATGGCGTCGACCTCTGCAGACCTGTGGAC
  TCAGAGGCCACGGCGGGGGCCCTCTCCAATGGCCCAGACTGCACTCCCCCTGCCAACGCCGCCTCCACAC
  CCGCCCCTTCCCCCTCCTCGCAGAGCTGCACAGCGAACTGTAACCAGGGGGAAGAGACTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223342 representing NM_001109691
  Red=Cloning site Green=Tags(s)
  
  MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLIVKQEQPD
  KFQIQPLSQSENKLQTAQQQPLQPLQQQQPQQPQQQQQQQQQHAQQSAAAPPSLTASQQVQATPPQPIKV
  PQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQNSIHPVR
  VVNGQTATIAKTFPMAQLTSIVIATPGTRLPGPQTVQLSKPSLEKQTVKSHPEAEEKQAESRTVTPPAAP
  KPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNSTMHPGT
  RKRGRPPKYNAVLGFGALTPTSPPSSHPDSPENEKTETTFTFPAPVQPVSLPSPTSTDGDIHEDFCSVCR
  KSGQLLMCDTCSRVYHLDCLEPPLKTIPKGMWICPRCQDQMLKKEEAIPWPGTLAIVHSYIAYKAAKEEE
  KQKLLKWSSDLKQEREQLEQKVKELSSSISKCMEMKSSILARQKEMRSSLDKVKRLIRLVHGVDLCRPVD
  SEATAGALSNGPDCTPPANAASTPAPSPSSQSCTANCNQGEETK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109691
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109691.1, NP_001103161.1
• RefSeq Size: 6484 bp
• RefSeq ORF: 1815 bp
• Locus ID: 192285
• UniProt ID: Q6ZPK0
• Cytogenetics: 2 E1
• MW: 67.5 kDa
• Gene Summary: Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation (By similarity).[UniProtKB/Swiss-Prot Function]