Tbata (NM_001017419) Mouse Tagged ORF Clone
CAT#: MR223308
- TrueORF®
Tbata (Myc-DDK-tagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001017419" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tbata |
Synonyms | 1700021K02Rik; AI428928; S; Spatial; Titest |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223308 representing NM_001017419
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACAGAGGTGAACCAGCTGTCTGAGCATCCTCTAGTGAGTCCAAAGGCAGAGCCCCAGCCAGAGA CGAAGCCGGAGAACCTTCCAAGGAGCCACGGGGATGTTGGGCTCCAGAAAGAGACTGTGGTCCCAGGCAT TGTGGATTTCGAGCTGATCCATGAGGAGCTGAAGACCACAAAGCCCCAAACATCACAACCAACACCCAGT GCCTACCGCTTTGGACGCCTAAGCCACCATTCCTTCTTCTCGAGGCACCACCCCCAACCACAGCGAGTGA CTCATATCCAAGATATCGCTGGGAAGCCTGTCTGCGTGGTCAGGGACGAGTTCTCTCTGTCGGCCTTGAC TCAGCCCACATTCTTATCCCGCTGTCTGATGGGGATGCCCACCATCTCTGTCCCCATTGGGGATCCACAG TCCAATCGGAACCCCCAGCTTTCTACTTCTGACACCTGGAGGAAGAAACTGAAGGACCTGGCTTCCCGAG TGACTGTCTTCACTAAGGAAATCCAGCCAAAGCCCGATGAGCAGAAGGAAGAGCCACCTCTGAGGGAACC TCCTCCGAGAGAGCAGGGGGCCAAATACTCAGCTGAGACCGGTAGGCTTATCCCTGCTTCCAGCCAAGCC CTCACCCGTCGCAACCGCCAGGGCCAGCGGGTCCACCCTTCTAGCAAAGATGGAGGAGTCCAAGCCTCCA TTCTGCAGGACCAGGAGCTGCTGATTTTGGAGCTCCTTTGTCAAATTCTACAAACAGATTCTCTAAGGGC TATTCAGTTCTGGCTGCTTTATGCTCCATCAAAAGAAAAAGATTTAGCTCTGGGGCTTCTGCAAACTGCC GTGGCTCAGCTTATCCCCCAGCCCCTTTCCTCCATCCCAGCAGAGAAGCTCTGGAACCATCTCCAAGAGC TTCAAGAGCCTCAAGAGACACAAGAGGCAGCCTACAGTCCATCCCTGAAGAAGACTAGGTCACCACCTCT ACCCAAAACAGACAAACCAGAGTACATAGGCAAAGCCCAAGTCCTCCTGGTGCATCCCAGCGAGGACCCA GAGGAGAAAACGACCAAGGCTGAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223308 representing NM_001017419
Red=Cloning site Green=Tags(s) MTTEVNQLSEHPLVSPKAEPQPETKPENLPRSHGDVGLQKETVVPGIVDFELIHEELKTTKPQTSQPTPS AYRFGRLSHHSFFSRHHPQPQRVTHIQDIAGKPVCVVRDEFSLSALTQPTFLSRCLMGMPTISVPIGDPQ SNRNPQLSTSDTWRKKLKDLASRVTVFTKEIQPKPDEQKEEPPLREPPPREQGAKYSAETGRLIPASSQA LTRRNRQGQRVHPSSKDGGVQASILQDQELLILELLCQILQTDSLRAIQFWLLYAPSKEKDLALGLLQTA VAQLIPQPLSSIPAEKLWNHLQELQEPQETQEAAYSPSLKKTRSPPLPKTDKPEYIGKAQVLLVHPSEDP EEKTTKAES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001017419 |
ORF Size | 1077 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001017419.2, NP_001017419.1 |
RefSeq Size | 1443 bp |
RefSeq ORF | 1080 bp |
Locus ID | 65971 |
UniProt ID | Q7TSD4 |
Cytogenetics | 10 B4 |
MW | 40.7 kDa |
Gene Summary | This gene encodes a putative transcription factor that is highly expressed in thymic cortical stromal cells, and may be involved in T-cell development. Its expression is developmentally regulated in the testis, where it is restricted to the haploid round spermatids during spermatogenesis, and thus this gene may also have a role in the control of male germ cell development. Alternative splicing of this gene results in two sets of transcript variants: the variants containing 5 additional exons at the 3' end encode long isoforms that are highly expressed in the testis, while the variants lacking the 3' end exons encode short isoforms that are highly expressed in the thymus. Most of the transcripts encoding the short isoforms have been shown to initiate translation from non-AUG (CUG) start sites. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210250 | Tbata (untagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 2, (10ug) |
USD 503.00 |
|
MG223308 | Tbata (tGFP-tagged) - Mouse thymus brain and testes associated (Tbata) transcript variant 2, (10ug) |
USD 703.00 |
|
MR223308L3 | Lenti ORF clone of Tbata (Myc-DDK-tagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 2 |
USD 803.00 |
|
MR223308L4 | Lenti ORF clone of Tbata (mGFP-tagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 2 |
USD 803.00 |
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