Neu3 (NM_016720) Mouse Tagged ORF Clone

CAT#: MR223297

Neu3 (Myc-DDK-tagged) - Mouse neuraminidase 3 (Neu3)

Product Images

Circular map for MR223297

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neu3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223297 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAGTCCCACCCTACTCCCTCAGCAGCACCCTGTTCCAGCAGGAAGAACAGAGTGGGGTGACCT
  ACCGGATCCCAGCCCTGCTGTACCTTCCTCCCACCCACACCTTCCTGGCCTTTGCAGAGAAGCGGACCTC
  AGTCAGAGATGAGGATGCTGCCTGCCTGGTGCTCAGACGAGGGCTGATGAAGGGGCGCTCTGTACAGTGG
  GGCCCCCAACGGCTACTGATGGAGGCCACATTACCTGGGCATCGCACCATGAACCCCTGCCCTGTGTGGG
  AGAAAAATACTGGCCGTGTGTACCTGTTTTTCATCTGTGTGCGGGGCCATGTTACTGAGAGGTGCCAGAT
  TGTGTGGGGCAAAAATGCCGCCCGTCTCTGCTTCCTTTGCAGTGAAGATGCCGGCTGCTCTTGGGGTGAA
  GTGAAAGACTTGACCGAGGAGGTCATTGGCTCAGAGGTGAAGCGCTGGGCCACATTTGCTGTGGGCCCAG
  GTCATGGCATCCAGCTACACTCGGGAAGGCTGATCATCCCCGCCTATGCCTACTATGTCTCACGTTGGTT
  TCTCTGCTTTGCGTGTTCAGTCAAGCCCCATTCCCTGATGATCTACAGTGATGACTTTGGAGTCACATGG
  CACCATGGCAAGTTCATTGAGCCCCAGGTGACAGGGGAGTGCCAAGTGGCCGAAGTGGCTGGGACGGCTG
  GTAACCCTGTGCTCTACTGCAGTGCCCGAACACCAAGCCGATTTCGAGCAGAGGCTTTTAGTACTGATAG
  TGGTGGCTGCTTTCAGAAGCCAACCCTGAACCCACAACTCCATGAGCCTCGAACCGGCTGCCAAGGTAGT
  GTAGTGAGCTTCCGGCCTTTGAAGATGCCAAATACCTATCAAGACTCAATTGGCAAAGGTGCTCCCGCTA
  CTCAGAAGTGCCCTCTGCTGGACAGTCCTCTGGAGGTGGAGAAAGGAGCTGAAACACCATCAGCAACATG
  GCTCTTGTACTCACATCCAACTAGCAAGAGGAAGAGGATTAACCTAGGCATCTACTACAACCGGAACCCC
  TTGGAGGTGAACTGCTGGTCCCGCCCGTGGATCTTGAACCGTGGGCCCAGTGGCTACTCTGATCTGGCTG
  TTGTGGAAGAACAGGACTTGGTGGCGTGTTTGTTTGAGTGTGGGGAGAAGAATGAGTATGAGCGGATTGA
  CTTCTGTCTGTTTTCAGACCACGAGGTCCTGAGCTGTGAAGACTGTACCAGCCCTAGTAGCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223297 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEVPPYSLSSTLFQQEEQSGVTYRIPALLYLPPTHTFLAFAEKRTSVRDEDAACLVLRRGLMKGRSVQW
  GPQRLLMEATLPGHRTMNPCPVWEKNTGRVYLFFICVRGHVTERCQIVWGKNAARLCFLCSEDAGCSWGE
  VKDLTEEVIGSEVKRWATFAVGPGHGIQLHSGRLIIPAYAYYVSRWFLCFACSVKPHSLMIYSDDFGVTW
  HHGKFIEPQVTGECQVAEVAGTAGNPVLYCSARTPSRFRAEAFSTDSGGCFQKPTLNPQLHEPRTGCQGS
  VVSFRPLKMPNTYQDSIGKGAPATQKCPLLDSPLEVEKGAETPSATWLLYSHPTSKRKRINLGIYYNRNP
  LEVNCWSRPWILNRGPSGYSDLAVVEEQDLVACLFECGEKNEYERIDFCLFSDHEVLSCEDCTSPSSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016720
• ORF Size: 1257 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016720.1
• RefSeq Size: 3341 bp
• RefSeq ORF: 1257 bp
• Locus ID: 50877
• UniProt ID: Q9JMH7
• Cytogenetics: 7 E1
• MW: 46.8 kDa
• Gene Summary: Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.[UniProtKB/Swiss-Prot Function]