Acin1 (NM_001085473) Mouse Tagged ORF Clone
CAT#: MR223239
- TrueORF®
Acin1 (Myc-DDK-tagged) - Mouse apoptotic chromatin condensation inducer 1 (Acin1), transcript variant 4
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001085473" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Acin1 |
Synonyms | 2610036I19Rik; 2610510L13Rik; Acinus; acinusL; acinusS; Acn; C79325; mKIAA0670 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223239 representing NM_001085473
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGACGGAAACGACCGAACAGCTCCGGAGAAACCCGGGGAATTCTGAGCGGTAATAGAGGGGTAG ACTATGGCAGTGGGCGGGGCCAATCCGGTCCGTTCGAAGGGCGTTGGCGGAAATTACCGAAGATGCCCGA AGCCGTCGGGACGGACCCGAGTACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTGGACGGGAAG CCTCTTCAGGCTCTGCGGGTGACCGACCTGAAGGCCGCACTGGAGCAGCGAGGCCTAGCCAAGAGCGGGC AGAAGAGTGCCCTGGTCAAGCGGCTCAAAGGGGCTCTAATGCTAGAAAATTTACAAAAACACTCAACACC CCATGCTGCATTCCAGCCAAATTCACAGATCGGGGAGGAAATGAGCCAGAACAGCTTCATAAAGCAGTAT CTGGAAAAGCAGCAGGAGCTACTTAGGCAGCGCCTGGAACGGGAAGCTCGGGAGGCTGCGGAACTTGAAG AAGCCTCAGCTGAGTCGGAGGACGAGATGACCCATCCTGAGGGAGTGGCTTCCCTGCTGCCTCCTGATTT TCAGAGCAGCCTGAACAGACCAGAGCTGGAGCTCAGCACACATTCACCCCGAAAGAGCTCCTCTTTTTCT GAAGAAAAAGGAGAATCTGATGATGAGAAGCCGAGGAAAGGGGAAAGACGATCATCCAGAGTTAGACAGG CAAAATCTAAACTCCCTGAGTACAGTCAGACTGCTGAGGAGGAAGAGGATCAAGAGACACCATCTAGAAA CCTGAGGGTCAGAGCAGATCGAAATTTAAAAATAGAGGAGGAGGAAGAAGAAGAGGAAGAAGAAGAAGAT GACGATGATGAAGAGGAAGAGGAGGTAGATGAGGCACAGAAATCTAGAGAGGCTGAGGCACCAACCCTGA AGCAGTTTGAGGATGAAGAAGGGGAAGAGAGGACCAGAGCAAAACCAGAAAAGGTGGTGGATGAGAAACC CCTAAACATCAGATCCCAGGAAAAGGGTGAGTTAGAGAAAGGAGGGCGAGTTACTAGATCCCAAGAAGAA GCTAGAAGAAGTCATCTGGCCAGACAGCAACAAGAGAAAGAGACACAAATTGTTTCTCTCCCTCAAGAAG AAAATGAGGTAAAGTCTTCACAAAGTTTGGAGGAAAAATCCCAGTCTCCCTCCCCACCTCCGCTTCCTGA AGACCTAGAGAAGGCCCCTGTTGTGCTGCAGCCAGAACAGATAGTCAGTGAAGAGGAAACACCCCCACCT TTACTTACCAAGGAAGCGTCATCTCCACCAACTCATATACAACTCCAGGAAGAGATGGAGCCAGTGGAAG GCCCAGCACCCCCTGTCCTCATTCAGTTGTCTCCTCCTAACACAGATGCTGGAGCCAGGGAGCCACTAGC GTCTCCTCACCCTGCGCAGCTGCTAAGAAGCCTGTCTCCTTTGTCAGGTACTACAGACACCAAAGCAGAG TCTCCAGCAGGGAGAGTGTCAGATGAGAGTGTCCTGCCTCTGGCTCAGAAAAGCTCACTGCCTGAATGCT CCACCCAGAAGGGTGTTGAAAGTGAGCGTGAAAAGTCTGCTCCCCTCCCTCTAACAGTGGAGGAGTTCGC ACCTGCCAAAGGCATCACTGAGGAACCTATGAAGAAACAGTCTTTGGAACAGAAGGAGGGCAGAAGAGCC TCCCATGCTCTCTTCCCAGAGCACAGTGGGAAGCAGTCAGCCGACTCATCCTCTAGCAGGTCCTCCTCCC CTTCATCCTCCAGCTCTCCATCCAGATCTCCATCTCCTGACAGTGTGGCTTCCCGCCCACAGTCGTCTCC AGGATCTAAGCAGAGAGATGGAGCCCAAGCACGAGTTCATGCCAACCCTCACGAGAGACCCAAGATGGGC TCCAGGTCAACTTCAGAGTCCAGGTCACGTTCCCGATCTCGTTCCCGTTCCGCGTCAAGCAGCAGCAGAA AATCTCTGAGCCCTGGAGTCTCCAGAGATAGCAACACCAGCTACACTGAAACCAAAGATCCCTCTTGTGG TCAGGAGGCTGCAGCTCCATCTGGGCCACAGCTGCAGGTCCTTGAACCAAAGGAGAAGGCTCCCACGTTC TCAGCATCTGTCCGGGGGAGGCATCTGAGCCATCCTGAGCCAGAGCAACAGCACGTGATCCAGAGGTTGC AGCCGGAGCAGGGGAGCCCAAAGAAGTGTGAAGCTGAAGAGGCAGAGCCACCAGCTGCCACACAGCCTCA AACCTCAGAGACTCAGATCTCTCATCTGCTAGAATCAGAAAGGACTCATCACACTGTTGAAGAGAAGGAG GAAGTGACCATGGACACCAGTGAAAACAGACCTGAAAATGAGGTGCCTGAGCCTCCTCTGCCTGTTGCAG ACCAAGTCAGCAATGATGAGCGCCCAGAGGGTGGTGCTGAAGAAGAGGAAAAGAAAGAGAGTTCGATGCC CAAGTCATTCAAGAGGAAAATCTCCGTTGTCTCAGCTACCAAGGGGGTGCAAGCTGGAAACAGTGACACA GAGGGGGGCCAGCCTGGCCGAAAACGCCGTTGGGGAGCCAGCACTGCCGCGACACAGAAGAAACCGTCCA TCAGTATCACCACTGAGTCACTCAAGGAGGCTGTTGTGGATCTTCATGCCGATGACTCCCGAATCTCTGA GGATGAGACAGAGCGTAATGGCGACGATGGGACCCATGACAAGGGACTGAAGATATGCCGGACAGTCACT CAGGTAGTACCCGCAGAGGGCCAGGAGAATGGGCAGAGGGAAGAGGAAGAAGAGAAAGAGCCTGAAGCCG AGCTGCCGGCGCCACCCCAGGTGTCAGTGGAGGTTGCCTTGCCCCCACCTGTGGAGCACGAAGTAAAGAA AGTAACATTAGGAGATACCTTAACCCGGAGGTCCATCAGCCAACAGAAGTCTGGAGTTTCCATTACAATT GATGACCCAGTCCGGACCGCCCAGGTGCCCTCCCCACCCAGGGGCAAGATCAGTAACATTGTCCACATCT CCAACCTGGTTCGTCCCTTCACTTTAGGCCAGCTGAAGGAATTATTGGGGCGTACAGGAACTTTGGTGGA AGAGGCCTTCTGGATAGACAAGATCAAATCTCATTGCTTTGTGACGTACTCTACAGTAGAGGAAGCCGTT GCCACCCGCACAGCTCTGCACGGGGTCAAGTGGCCCCAGTCCAACCCCAAATTCCTTTGTGCTGACTATG CTGAGCAAGATGAGCTGGACTATCACCGGGGACTCTTGGTAGATCGGCCATCTGAAACTAAGGCAGAGGA ACAGGGAGCACCAAGGCCCCTGCATCCCCCACCCCCACCCCCAGTCCAGCCACCGCCCCACCCCCGGGCT GAGCAGCGGGAGCAGGAAAGGGCTGTTCGAGAGCAATGGGCAGAACGGGAACGGGAAATGGAGCGCCGGG AGAGGACTCGGTCTGAGAGAGAATGGGATCGGGACAAAGTTCGAGAGGGACCCCGCTCCCGATCACGGTC CCGTGACCGCCGCCGGAAAGAGCGAGCAAAATCTAAAGAAAAGAAGAGTGAAAAGAAAGAAAAAGCCCAG GAGGAGCCACCTGCCAAGCTGCTGGATGACCTCTTCCGTAAGACTAAGGCAGCTCCCTGCATCTATTGGC TCCCTCTGACTGAGAGCCAAATTGTTCAGAAGGAGGCAGAGCAAGCTGAACGGGCCAAGGAGCGGGAGAA GCGGCGAAAAGAACGAGAAGAAGAAGAACAAAAGGAACGGGAGAAGGAAGCTGAGCGGGAACGGAACCGG CAGCTAGAACGGGAGAAGAGGAGGGAGCACAGCAGGGAGAGAGAGAGGGACAGGGAGAGAGAGCGGGACA GGGGTGACCGAGAGCGGGAGAGGGAGCGAGACCGAGACCGAGGCAGGGAGAGGGATCGCAGAGACACCAA GCGCCACAGCAGAAGCCGGAGTCGAAGCACACCTGTACGGGACCGGGGTGGGCGCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223239 representing NM_001085473
Red=Cloning site Green=Tags(s) MWGRKRPNSSGETRGILSGNRGVDYGSGRGQSGPFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGK PLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQY LEKQQELLRQRLEREAREAAELEEASAESEDEMTHPEGVASLLPPDFQSSLNRPELELSTHSPRKSSSFS EEKGESDDEKPRKGERRSSRVRQAKSKLPEYSQTAEEEEDQETPSRNLRVRADRNLKIEEEEEEEEEEED DDDEEEEEVDEAQKSREAEAPTLKQFEDEEGEERTRAKPEKVVDEKPLNIRSQEKGELEKGGRVTRSQEE ARRSHLARQQQEKETQIVSLPQEENEVKSSQSLEEKSQSPSPPPLPEDLEKAPVVLQPEQIVSEEETPPP LLTKEASSPPTHIQLQEEMEPVEGPAPPVLIQLSPPNTDAGAREPLASPHPAQLLRSLSPLSGTTDTKAE SPAGRVSDESVLPLAQKSSLPECSTQKGVESEREKSAPLPLTVEEFAPAKGITEEPMKKQSLEQKEGRRA SHALFPEHSGKQSADSSSSRSSSPSSSSSPSRSPSPDSVASRPQSSPGSKQRDGAQARVHANPHERPKMG SRSTSESRSRSRSRSRSASSSSRKSLSPGVSRDSNTSYTETKDPSCGQEAAAPSGPQLQVLEPKEKAPTF SASVRGRHLSHPEPEQQHVIQRLQPEQGSPKKCEAEEAEPPAATQPQTSETQISHLLESERTHHTVEEKE EVTMDTSENRPENEVPEPPLPVADQVSNDERPEGGAEEEEKKESSMPKSFKRKISVVSATKGVQAGNSDT EGGQPGRKRRWGASTAATQKKPSISITTESLKEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVT QVVPAEGQENGQREEEEEKEPEAELPAPPQVSVEVALPPPVEHEVKKVTLGDTLTRRSISQQKSGVSITI DDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAV ATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKAEEQGAPRPLHPPPPPPVQPPPHPRA EQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQ EEPPAKLLDDLFRKTKAAPCIYWLPLTESQIVQKEAEQAERAKEREKRRKEREEEEQKEREKEAERERNR QLEREKRREHSRERERDRERERDRGDRERERERDRDRGRERDRRDTKRHSRSRSRSTPVRDRGGRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001085473 |
ORF Size | 3978 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001085473.2, NP_001078942.2 |
RefSeq Size | 4715 bp |
RefSeq ORF | 3981 bp |
Locus ID | 56215 |
UniProt ID | Q9JIX8 |
Cytogenetics | 14 C2 |
MW | 149.9 kDa |
Gene Summary | Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224239 | Acin1 (untagged) - Mouse apoptotic chromatin condensation inducer 1 (Acin1), transcript variant 4, (10ug) |
USD 1,270.00 |
|
MG223239 | Acin1 (tGFP-tagged) - Mouse apoptotic chromatin condensation inducer 1 (Acin1) transcript variant 4, (10ug) |
USD 1,469.00 |
|
MR223239L3 | Lenti ORF clone of Acin1 (Myc-DDK-tagged) - Mouse apoptotic chromatin condensation inducer 1 (Acin1), transcript variant 4 |
USD 1,569.00 |
|
MR223239L4 | Lenti ORF clone of Acin1 (mGFP-tagged) - Mouse apoptotic chromatin condensation inducer 1 (Acin1), transcript variant 4 |
USD 1,569.00 |
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