Prg4 (NM_001110146) Mouse Tagged ORF Clone
CAT#: MR222669
- TrueORF®
Prg4 (Myc-DDK-tagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001110146" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Prg4 |
Synonyms | CACP; DOL54; JCAP; lubricin; MSF; SZP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222669 representing NM_001110146
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTGGAAAATACTTCCCGTCTGCTTGTCGCTGCTGCTTCCTGTTGTCTTGATTCAACAGGTTTCAT CTCAAGATTTATCAAGCTGTGCAGGGAGATGTGGGGAAGGGTACTCTAGAGATGCCACCTGCAACTGTGA TTATAACTGTCAACACTACATGGAGTGCTGTCCTGATTTCAAGAGAGTCTGCACTACAGAGCTCTCCTGC AAAGGACGCTGCTTCGAGTCCTTCGCTCGAGGGAGGGAGTGTGACTGTGACTCCCAGTGTAAACAGTATG GCAAGTGCTGTGCAGATTATGACAGCTTCTGTGAAGAAGTAAAAGATAACAAGAAAAACACTCCTAAAAA GAAACCCAATCCAGAACCGCCGGCTGTGGATGAAGCTGGAAGCGGGCTGGATAATGCTATTCCAGGCACT GATCTTTTGGCCGGGAGACTCAATCAAGGCATTAACATCAATCCCATGCTTTCAGATGAGACCAATTTAT GCAATGGTAAGCCAGTGGATGGACTGACTACGCTGCGCAATGGGACATTAGTTGCATTTCGAGGTCATTA TTTCTGGATGCTGAATCCATTCAGACCACCATCTCCACCACGCAGAATTACCGAAGTTTGGGGTATTCCC TCTCCTATTGACACAGTTTTTACTAGATGTAACTGTGAAGGAAAAACTTTCTTCTTTAAGGATTCTCAGT ACTGGCGCTTCACCAATGATGTGGTAGATCCTGGGTATCCTAAACAAATTGTCAAAGGCTTTGGAGGACT AACAGGGAAGATAGTGGCTGCTCTTTCAATAGCCAAGTACAAGGACAGGCCTGAATCTGTGTACTTCTTC AAGAGAGGTGGCAACATTCAGCAGTACACTTATAAACAGGAGCCAATGAAGAAGTGCACAGGGAGGCGGC CTGCCATCAACTACTCGGTGTACGGGGAGGCAGCACAGGTCAGGAGGCGCCGCTTTGAGCGTGCTGTTGG ACCTTTCCAGACACACACCTTCAGGATCCATTACTCGGTGCCCATGAGAGTTTCTTATCAAGACAAAGGT TTCCTCCACAATGAAGTCAAAGTGAGTACAATGTGGAGAGGGTTTCCAAATGTTGTTACTTCAGCGATAA CACTGCCTAACATTAGGAAACCTGATGGCTATGATTACTACGCCTTTTCCAAAGATCAATACTATAACAT TGATGTGCCTACAAGAACAGCAAGAGCAATTACCACACGTTCAGGGCAGACCCTCTCCAAAATCTGGTAC AACTGTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222669 representing NM_001110146
Red=Cloning site Green=Tags(s) MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSC KGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNAIPGT DLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIP SPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFF KRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKG FLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWY NCP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001110146 |
ORF Size | 1269 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001110146.1, NP_001103616.1 |
RefSeq Size | 1884 bp |
RefSeq ORF | 1272 bp |
Locus ID | 96875 |
UniProt ID | Q9JM99 |
Cytogenetics | 1 G1 |
MW | 48.5 kDa |
Gene Summary | Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212019 | Prg4 (untagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2, (10ug) |
USD 503.00 |
|
MG222669 | Prg4 (tGFP-tagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor articular superficial zone protein) (Prg4) transcript variant 2, (10ug) |
USD 703.00 |
|
MR222669L3 | Lenti ORF clone of Prg4 (Myc-DDK-tagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2 |
USD 803.00 |
|
MR222669L4 | Lenti ORF clone of Prg4 (mGFP-tagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2 |
USD 803.00 |
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