Prg4 (NM_001110146) Mouse Tagged ORF Clone

CAT#: MR222669

Prg4 (Myc-DDK-tagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2

Product Images

Circular map for MR222669

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prg4
• Synonyms: CACP; DOL54; JCAP; lubricin; MSF; SZP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222669 representing NM_001110146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGGAAAATACTTCCCGTCTGCTTGTCGCTGCTGCTTCCTGTTGTCTTGATTCAACAGGTTTCAT
  CTCAAGATTTATCAAGCTGTGCAGGGAGATGTGGGGAAGGGTACTCTAGAGATGCCACCTGCAACTGTGA
  TTATAACTGTCAACACTACATGGAGTGCTGTCCTGATTTCAAGAGAGTCTGCACTACAGAGCTCTCCTGC
  AAAGGACGCTGCTTCGAGTCCTTCGCTCGAGGGAGGGAGTGTGACTGTGACTCCCAGTGTAAACAGTATG
  GCAAGTGCTGTGCAGATTATGACAGCTTCTGTGAAGAAGTAAAAGATAACAAGAAAAACACTCCTAAAAA
  GAAACCCAATCCAGAACCGCCGGCTGTGGATGAAGCTGGAAGCGGGCTGGATAATGCTATTCCAGGCACT
  GATCTTTTGGCCGGGAGACTCAATCAAGGCATTAACATCAATCCCATGCTTTCAGATGAGACCAATTTAT
  GCAATGGTAAGCCAGTGGATGGACTGACTACGCTGCGCAATGGGACATTAGTTGCATTTCGAGGTCATTA
  TTTCTGGATGCTGAATCCATTCAGACCACCATCTCCACCACGCAGAATTACCGAAGTTTGGGGTATTCCC
  TCTCCTATTGACACAGTTTTTACTAGATGTAACTGTGAAGGAAAAACTTTCTTCTTTAAGGATTCTCAGT
  ACTGGCGCTTCACCAATGATGTGGTAGATCCTGGGTATCCTAAACAAATTGTCAAAGGCTTTGGAGGACT
  AACAGGGAAGATAGTGGCTGCTCTTTCAATAGCCAAGTACAAGGACAGGCCTGAATCTGTGTACTTCTTC
  AAGAGAGGTGGCAACATTCAGCAGTACACTTATAAACAGGAGCCAATGAAGAAGTGCACAGGGAGGCGGC
  CTGCCATCAACTACTCGGTGTACGGGGAGGCAGCACAGGTCAGGAGGCGCCGCTTTGAGCGTGCTGTTGG
  ACCTTTCCAGACACACACCTTCAGGATCCATTACTCGGTGCCCATGAGAGTTTCTTATCAAGACAAAGGT
  TTCCTCCACAATGAAGTCAAAGTGAGTACAATGTGGAGAGGGTTTCCAAATGTTGTTACTTCAGCGATAA
  CACTGCCTAACATTAGGAAACCTGATGGCTATGATTACTACGCCTTTTCCAAAGATCAATACTATAACAT
  TGATGTGCCTACAAGAACAGCAAGAGCAATTACCACACGTTCAGGGCAGACCCTCTCCAAAATCTGGTAC
  AACTGTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222669 representing NM_001110146
  Red=Cloning site Green=Tags(s)
  
  MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSC
  KGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNAIPGT
  DLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIP
  SPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFF
  KRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKG
  FLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWY
  NCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110146
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110146.1, NP_001103616.1
• RefSeq Size: 1884 bp
• RefSeq ORF: 1272 bp
• Locus ID: 96875
• UniProt ID: Q9JM99
• Cytogenetics: 1 G1
• MW: 48.5 kDa
• Gene Summary: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.[UniProtKB/Swiss-Prot Function]