Nkx2 (NM_001146198) Mouse Tagged ORF Clone
CAT#: MR222563
- TrueORF®
Nkx2 (Myc-DDK-tagged) - Mouse NK2 homeobox 1 (Nkx2-1), transcript variant 2
ORF Plasmid: tGFP
AAV Particle: DDK
"NM_001146198" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Nkx2 |
Synonyms | AV026640; Nkx2.1; T/EBP; Titf1; Ttf-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222563 representing NM_001146198
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGATGAGTCCAAAGCACACGACTCCGTTCTCAGTGTCTGACATCTTGAGTCCCCTGGAGGAAAGCT ACAAGAAAGTGGGCATGGAGGGCGGCGGCCTCGGGGCTCCGCTCGCAGCGTACAGACAGGGCCAGGCGGC CCCACCGGCCGCGGCCATGCAGCAGCACGCCGTGGGGCACCACGGCGCCGTCACCGCCGCCTACCACATG ACGGCGGCGGGGGTGCCCCAGCTCTCGCACTCCGCCGTGGGGGGCTACTGCAACGGCAACCTGGGCAACA TGAGCGAGCTGCCGCCTTACCAGGACACCATGCGGAACAGCGCTTCGGGCCCCGGATGGTACGGCGCCAA CCCAGACCCGCGCTTCCCCGCCATCTCCCGCTTCATGGGCCCGGCGAGCGGCATGAATATGAGTGGCATG GGCGGCCTGGGCTCGCTGGGGGACGTGAGCAAGAACATGGCCCCGCTGCCCAGTGCGCCCCGCCGGAAGC GCCGGGTGCTCTTCTCCCAGGCGCAGGTGTACGAGCTCGAGCGACGTTTCAAGCAACAGAAGTACCTGTC GGCGCCGGAGCGCGAGCATCTGGCCAGCATGATTCACCTGACACCCACGCAGGTCAAGATCTGGTTCCAG AACCACCGCTACAAGATGAAGCGCCAGGCTAAGGACAAGGCGGCGCAGCAACAACTGCAGCAGGACAGCG GCGGCGGCGGAGGCGGCGGTGGCGGTGCGGGATGCCCGCAGCAGCAGCAAGCTCAGCAGCAGTCGCCGCG CCGGGTGGCCGTGCCGGTCCTAGTCAAAGACGGCAAACCCTGCCAGGCGGGCGCCCCTGCCCCGGGAGCC GCAAGCCTGCAAAGCCACGCGCAGCAACAAGCTCAGCAGCAGGCGCAGGCGGCGCAAGCGGCTGCCGCGG CCATCTCAGTGGGCAGCGGTGGCGCGGGTCTAGGAGCACACCCAGGCCACCAGCCGGGCAGCGCAGGGCA GTCCCCGGACCTGGCGCACCACGCAGCCAGCCCCGCGGGGCTGCAGGGCCAGGTCTCCAGCCTATCCCAT CTGAACTCCTCGGGCTCGGACTATGGCGCCATGTCTTGTTCTACCTTGCTTTATGGTCGGACCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222563 representing NM_001146198
Red=Cloning site Green=Tags(s) MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPAAAMQQHAVGHHGAVTAAYHM TAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGM GGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQ NHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGGAGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGA ASLQSHAQQQAQQQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAGLQGQVSSLSH LNSSGSDYGAMSCSTLLYGRTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001146198 |
ORF Size | 1116 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146198.1, NP_001139670.1 |
RefSeq Size | 2265 bp |
RefSeq ORF | 1119 bp |
Locus ID | 21869 |
Cytogenetics | 12 24.42 cM |
MW | 38.6 kDa |
Gene Summary | Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation (PubMed:22955271). Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (PubMed:22356123).[UniProtKB/Swiss-Prot Function] |
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