Eda2r (NM_001161432) Mouse Tagged ORF Clone
CAT#: MR222258
- TrueORF®
Eda2r (Myc-DDK-tagged) - Mouse ectodysplasin A2 receptor (Eda2r), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001161432" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Eda2r |
Synonyms | 9430060M22Rik; TNFRSF27; Xedar |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222258 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTGTCAAGAGAATGAGTACCGGGACCAATGGGGGCGCTGTGTCACCTGCCAACAATGTGGCCCTG GACAGGAGCTCTCGAAGGATTGTGGTTATGGAGAAGGTGGAGATGCACACTGCATAGTCTGCCCTCCCCG AAAGTATAAAAGCACTTGGGGACATCACAGATGTCAGACATGCATCACTTGTGCTGTCATCAATCGGGTC CAGAAGGCCAACTGCACAAATACCTCTAATGCTATCTGTGGAGACTGTCTGCCCAGGTTCTACCGAAAGA CACGCATTGGGGGCCTGCAGGACCAAGAATGCATCCCATGTACAAAGCAGACTCCTTCTTCCGAGGTTCA GTGTACCTTCCAGTTGAGCTTAGTGAAGGTAGATGCACACACTGTTCCCCCTAGGGAAGCCACACTTGTT GCACTGGTGGGAAGTCTGCTAGTGGTATTTGCTCTGGCCTTCCTTGGACTCTTCTTCCTTTATTGCAAGC AGATCTTCAACAGACATTGTCAATGCAGAGATTCGTTGCAATATGAGGCTGAAAAGACAGTGGAGGAGGA TTCTCTTTTCCCCGTACCACCTGGCCAGGAGACCAGTCCTGAATTTCCAGCTAATGAGGGCATCTTGGAG ATCAAACCACTTAACTCCATCCTGGATGATGACTGCAGCTCCACTCGCGGCTTCCCCACCCAGGAGTCCT TTACCATGGCCTCCTGTGCCTCAGAGAGCCATTCTCAATGGGTCCATACCCCAATTGAATGTACAGAGCT GGACCTGCAAAAGTTTTCCAGCTCTATCCCCTCTACTGGACCTGAAACCTTGAGAGAGAACACAGCTGAA CACTCAGGAGACAGGCTGGAGCTCTATGTACCTTTTGAAGTTCCCAGCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222258 protein sequence
Red=Cloning site Green=Tags(s) MDCQENEYRDQWGRCVTCQQCGPGQELSKDCGYGEGGDAHCIVCPPRKYKSTWGHHRCQTCITCAVINRV QKANCTNTSNAICGDCLPRFYRKTRIGGLQDQECIPCTKQTPSSEVQCTFQLSLVKVDAHTVPPREATLV ALVGSLLVVFALAFLGLFFLYCKQIFNRHCQCRDSLQYEAEKTVEEDSLFPVPPGQETSPEFPANEGILE IKPLNSILDDDCSSTRGFPTQESFTMASCASESHSQWVHTPIECTELDLQKFSSSIPSTGPETLRENTAE HSGDRLELYVPFEVPSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001161432 |
ORF Size | 894 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001161432.1, NP_001154904.1 |
RefSeq Size | 4798 bp |
RefSeq ORF | 894 bp |
Locus ID | 245527 |
UniProt ID | Q8BX35 |
Cytogenetics | X C3 |
MW | 33.1 kDa |
Gene Summary | Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC213218 | Eda2r (untagged) - Mouse ectodysplasin A2 receptor (Eda2r), transcript variant 1, (10ug) |
USD 480.00 |
|
MG222258 | Eda2r (tGFP-tagged) - Mouse ectodysplasin A2 receptor (Eda2r) transcript variant 1, (10ug) |
USD 650.00 |
|
MR222258L3 | Lenti ORF clone of Eda2r (Myc-DDK-tagged) - Mouse ectodysplasin A2 receptor (Eda2r), transcript variant 1 |
USD 750.00 |
|
MR222258L4 | Lenti ORF clone of Eda2r (mGFP-tagged) - Mouse ectodysplasin A2 receptor (Eda2r), transcript variant 1 |
USD 750.00 |
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