Nptxr (NM_030689) Mouse Tagged ORF Clone

CAT#: MR221892

Nptxr (Myc-DDK-tagged) - Mouse neuronal pentraxin receptor (Nptxr)

Product Images

Circular map for MR221892

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nptxr
• Synonyms: 1200009K17Rik; 1700036C17Rik; 5730406O18Rik; AI452369; AI785356; D15Bwg0580e; NP; Npcd; NPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221892 representing NM_030689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGGCGCCTGGGACTCACCTGCGCTGCTGCTGGAGCTGGAGGACGCGGTGCGCGCGCTGCGAG
  ACCGCATCGAGCGCATCGAGCAGGAGCTCCCAGCCCGTGGGAACATCTCCTCGGCGCCAGCTCCAGCCAT
  GCCCACCGCCCTGCACTCCAAGATGGACGAACTAGAAGGCCAGCTGCTGGCCAAGGTGCTGGCGCTGGAG
  AAGGAGCGAGCCGCCCTCAGTCACGGGAGCCACCAGCAACGACAGGAAGTGGAGAAGGAGCTGAATGCCC
  TGCAGGGTCGCGTGGCCGAGCTGGAGCACGGGTCCTCGGCCTACAGTCCCCCTGATGCCTTCAAGGTCAG
  CATCCCCATCCGCAACAACTACATGTACGCCCGCGTGCGCAAGGCGCTGCCGGAACTCTACGCCTTCACT
  GCCTGCATGTGGCTGCGCTCCAGGTCAGGTGGCTCCGGCCAGGGCACCCCCTTCTCCTACTCGGTGCCCG
  GGCAGGCCAATGAGATCGTGCTTCTGGAGGCCGGCCTTGAGCCAATGGAGCTGCTGATCAACGACAAGGT
  GGCCCAGCTGCCCCTGAGCCTGAAAGACAGCAACTGGCACCATATCTGCATCTCCTGGACTACAAGGGAT
  GGGCTGTGGTCTGCATACCAGGACGGAGAACTGCGGGGTTCTGGCGAGAACCTAGCAGCCTGGCACCCCA
  TCAAGCCACACGGCATCCTTATCCTGGGCCAAGAGCAGGATACCTTGGGAGGCCGATTTGACGCCACACA
  GGCCTTCGTTGGTGACATTGCACAGTTCAACCTGTGGGACCACGCTCTGACACCGGCCCAGGTGCTGGGC
  ATGGCCAACTGCACCGGGCCACTGATGGGCAATGTCCTCCCCTGGGAAGATAAGCTGGTAGAGGCCTTTG
  GAGGTGCGAAGAAGGCCGCCTTTGATGTGTGCAAGGGGAGGGCAAAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221892 representing NM_030689
  Red=Cloning site Green=Tags(s)
  
  MADGAWDSPALLLELEDAVRALRDRIERIEQELPARGNISSAPAPAMPTALHSKMDELEGQLLAKVLALE
  KERAALSHGSHQQRQEVEKELNALQGRVAELEHGSSAYSPPDAFKVSIPIRNNYMYARVRKALPELYAFT
  ACMWLRSRSGGSGQGTPFSYSVPGQANEIVLLEAGLEPMELLINDKVAQLPLSLKDSNWHHICISWTTRD
  GLWSAYQDGELRGSGENLAAWHPIKPHGILILGQEQDTLGGRFDATQAFVGDIAQFNLWDHALTPAQVLG
  MANCTGPLMGNVLPWEDKLVEAFGGAKKAAFDVCKGRAKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030689
• ORF Size: 1021 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030689.1, NM_030689.2, NM_030689.3, NM_030689.4, NP_109614.2
• RefSeq Size: 4973 bp
• RefSeq ORF: 1482 bp
• Locus ID: 73340
• Cytogenetics: 15 37.85 cM
• MW: 35 kDa
• Gene Summary: This gene encodes a protein similar to the rat neuronal pentraxin receptor. The rat pentraxin receptor is an integral membrane protein that is thought to mediate neuronal uptake of the snake venom toxin, taipoxin, and its transport into the synapses. Studies in rat indicate that translation of this mRNA initiates at a non-AUG (CUG) codon. This may also be true for mouse and human, based on strong sequence conservation amongst these species. [provided by RefSeq, Jul 2008]