Dmp1 (NM_016779) Mouse Tagged ORF Clone

CAT#: MR221826

Dmp1 (Myc-DDK-tagged) - Mouse dentin matrix protein 1 (Dmp1)

Product Images

Circular map for MR221826

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dmp1
• Synonyms: AG1; AV020965; Dmp; DMP-1; PP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221826 representing NM_016779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTGTCATTCTCCTTGTGTTCCTTTGGGGGCTGTCCTGTGCTCTCCCAGTTGCCAGATACCACA
  ATACTGAATCTGAAAGCTCTGAAGAGAGGACGGGTGATTTGGCTGGGTCACCACCACCACCCACGAACAG
  TGAGTCATCAGAAGAAAGTCAAGCTAGCCCAGAGGGACAGGCAAATAGTGACCACACGGACAGCAGTGAA
  TCTGGAGAGGAGCTGGGCTACGACAGAGGCCAGTACAGACCGGCTGGTGGACTCTCTAAGAGTACGGGGA
  CCGGCGCCGATAAGGAGGATGATGAAGACGACAGTGGAGATGATACCTTTGGCGATGAGGACAATGATCT
  AGGGCCCGAAGAAGGACAGTGGGGAGGACCCTCCAAACTGGACAGTGATGAGGACTCCACAGACACCACA
  CAGTCCAGTGAAGACAGCACCTCTCAAGAAAACAGTGCCCAAGATACCCCCAGCGACAGCAAAGACCACG
  ACAGTGAGGATGAGGCAGACAGCCGGCCTGAGGCAGGCGACTCCACTCAGGACAGTGAGAGTGAGGAACA
  GCGGGTGGGAGGTGGCAGCGAGGGGGAGAGTAGCCACGGGGACGGTTCTGAGTTCGATGATGAAGGGATG
  CAGAGCGACGACCCCGAGAGTACCAGGAGCGATCGAGGCCACGCCAGAATGAGCAGCGCTGGTATCAGGT
  CGGAAGAATCTAAAGGGGACCACGAGCCCACGAGCACTCAGGATTCAGATGACAGCCAGTCTGTGGAATT
  TTCAAGCAGGAAGTCCTTCAGAAGGTCCCACGTCTCTGAGGAAGACTACAGAGGTGAGCTTACTGACAGC
  AACAGCAGGGAAACCCAGAGCGACTCCACGGAGGATACGGCCTCCAAGGAGGAAAGCAGGAGCGAGTCTC
  AGGAGGACACAGCCGAGAGCCAGTCCCAGGAAGATAGCCCAGAGGGGCAAGACCCCAGCAGTGAGTCCAG
  CGAAGAGGCTGGTGAGCCATCCCAGGAAAGCAGCAGCGAATCTCAGGAAGGGGTGACCAGCGAGTCCAGG
  GGTGACAACCCAGATAACACAAGTCAGGCAGGAGACCAAGAAGACAGTGAGTCCAGTGAGGAGGACAGCC
  TGAACACATTCTCCAGCTCAGAAAGCCAGTCCACCGAGGAGCAAGCTGACAGCGAGTCCAACGAGAGCCT
  CAGCCTCTCCGAGGAGAGTCAGGAGTCGGCCCAGGATGGTGACAGCTCCAGCCAGGAAGGCCTGCAGTCC
  CAGAGCGCATCCACTGAGAGCAGGAGCCAGGAGAGCCAGTCTGAGCAGGACAGCCGTTCTGAGGAAGACA
  GTGACTCTCAGGACAGTAGCCGATCCAAAGAAGAGAGCAACTCCACAGGGAGCGCTTCCAGCAGCGAGGA
  GGACATCCGTCCCAAGAACATGGAAGCTGACAGTAGGAAACTAATAGTTGATGCTTACCACAACAAACCC
  ATCGGGGACCAAGATGACAATGACTGTCAGGACGGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221826 representing NM_016779
  Red=Cloning site Green=Tags(s)
  
  MKTVILLVFLWGLSCALPVARYHNTESESSEERTGDLAGSPPPPTNSESSEESQASPEGQANSDHTDSSE
  SGEELGYDRGQYRPAGGLSKSTGTGADKEDDEDDSGDDTFGDEDNDLGPEEGQWGGPSKLDSDEDSTDTT
  QSSEDSTSQENSAQDTPSDSKDHDSEDEADSRPEAGDSTQDSESEEQRVGGGSEGESSHGDGSEFDDEGM
  QSDDPESTRSDRGHARMSSAGIRSEESKGDHEPTSTQDSDDSQSVEFSSRKSFRRSHVSEEDYRGELTDS
  NSRETQSDSTEDTASKEESRSESQEDTAESQSQEDSPEGQDPSSESSEEAGEPSQESSSESQEGVTSESR
  GDNPDNTSQAGDQEDSESSEEDSLNTFSSSESQSTEEQADSESNESLSLSEESQESAQDGDSSSQEGLQS
  QSASTESRSQESQSEQDSRSEEDSDSQDSSRSKEESNSTGSASSSEEDIRPKNMEADSRKLIVDAYHNKP
  IGDQDDNDCQDGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016779
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016779.2, NP_058059.2
• RefSeq Size: 2760 bp
• RefSeq ORF: 1512 bp
• Locus ID: 13406
• UniProt ID: O55188
• Cytogenetics: 5 50.61 cM
• MW: 54.4 kDa
• Gene Summary: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity).[UniProtKB/Swiss-Prot Function]