Mfrp (NM_001190314) Mouse Tagged Lenti ORF Clone
CAT#: MR221748L3
- LentiORF®
Lenti ORF clone of Mfrp (Myc-DDK-tagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 2
"NM_001190314" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Mfrp |
Synonyms | rd6 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR221748).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001190314 |
ORF Size | 1737 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001190314.1, NP_001177243.1 |
RefSeq Size | 4274 bp |
RefSeq ORF | 1737 bp |
Locus ID | 259172 |
UniProt ID | Q8K480 |
Cytogenetics | 9 24.6 cM |
Gene Summary | The protein encoded by this gene contains a region with similarity to the cysteine-rich domain (CRD) of frizzled, a gene originally found in Drosophila that controls tissue polarity. This protein functions in eye development, where it is necessary for the maintenance of photoreceptor outer segments. Mutations in this gene cause retinal degeneration 6 in mice, which gives rise to a mouse model for human retinitis punctata albescens. Bicistronic transcripts composed of the coding sequences for this gene (Mfrp) and the C1q and tumor necrosis factor related protein 5 gene (C1qtnf5) have been identified, and the resulting products can interact with each other. Co-transcription of C1qtnf5 and Mfrp has been observed in both human and mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219233 | Mfrp (untagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 2, (10ug) |
USD 593.00 |
|
MG221748 | Mfrp (tGFP-tagged) - Mouse membrane-type frizzled-related protein (Mfrp) transcript variant 2, (10ug) |
USD 739.00 |
|
MR221748 | Mfrp (Myc-DDK-tagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 2 |
USD 539.00 |
|
MR221748L4 | Lenti ORF clone of Mfrp (mGFP-tagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 2 |
USD 839.00 |
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